Community Target Request
Do you have material and want to fast-track your request? Make sure you have all relevant clone and protein information before submitting the form.
Sequence Information
Organism:
Acanthamoeba
Anaplasma
Arenavirus
Babesia
Bartonella
Borrelia
Brucella
Burkholderia
Coccidioides
Cryptosporidium
Cyclospora
Ebola-like viruses
Ehrlichia
Encephalitozoon
Entamoeba
Erythrovirus
Filoviridae
Giardia
Hantavirus
Henipavirus
Homo
Influenzavirus
Lyssavirus
Marburgvirus
Mycobacterium
Nairovirus
Orthobunyavirus
Other
Phlebovirus
Rickettsia
Rubulavirus
Toxoplasma
UniProt ID:
UniProt description:
UniProt sequence:
Not in UniProt?
Hyperlink to webpage with sequence:
No identifier available?
Annotation:
DNA Sequence
Area of biomedical interest
Required: Make only 1 choice, select most relevant
Known drug target – The gene product is the target of a drug which is currently on the market or in development. Minimally, inhibitors of the target will have been shown to block cell growth in the genus/species listed.
Potential drug target – An ortholog of a known drug target, an essential gene or a homolog of an essential gene.
Targets associated with virulence and/or pathogenesis- A gene product associated with pathogen adhesion, colonization, invasion, immune response inhibition or toxicity of the host cells.
Vaccine candidate- A gene product identified as a vaccine candidate.
Targets involved in drug resistance- The gene product is confirmed or suggested to be involved (directly or indirectly) in drug-target binding, drug degradation or drug removal all of which result in evolution over time of drug resistance.
Targets showing phylogenetic evidence for essentiality, virulence or pathogenesis- Targets for which orthologues present in a pathogenic species/strain and absent in a non-pathogenic species/strain.
Targets associated with innate immunity- Human proteins associated with non-adaptive immune mechanisms that recognize, and respond to, microorganisms, microbial products and antigens.
Marker of infection- A biomarker, antigen or other protein used to detect pathogen infection.
Targets of other biological interest- targets falling outside the categories described above.
Unknown
Justification
Required: Include a brief (one or two sentence) description of how determination of the target structure will enhance research progress in the field.
Supporting Evidence
Describe supporting experimental evidence and/or relevant literature for functional or biological relevance of the target.
Material Availability
Clone name:
Full clone nucleotide sequence (vector + insert):
Does this construct express soluble protein?:
Yes
No
If yes, how much in mg/ml?
If no, do you have a solubilization protocol and if so, how much protein does the protocol produce in mg/ml?
List any important post-translational protein modifications, additives to improve solubility or purification, known ligands and their availability.
Full clone nucleotide sequence (vector + insert)
Expressed Protein sequence (including tag):
Vector
Tag
List any additional information, e.g. ligands or co-factors bound, etc
Can you provide a minimum of 150 ul at ~5mg/ml soluble protein?
Yes
No
List any important post-translational protein modifications, additives to improve solubility or purification, known ligands and their availability.
Applicant Information
Name (required)
Title
Affiliation
Address 1
Address 2
Address 3
Address 4
City (required)
State
Zip
Country (required)
Phone
Email (required)
Alternate contact
Alternate email
Intellectual Property
I understand that any structures successfully determined by the SSGCID will be submitted to the Protein Data Bank (http:/www.rcsb.org/pdb) and be part of the public domain.
Yes
No
Human Verification
4 + 1 =
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