NIAID Emerging and Re-emerging Infectious Diseases NIAID Bioinformatics Resource Centers NIAID Structural Genomic Centers Center for Structural Genomics of Infectious Disease
Seattle Structural Genomics Center for Infectious Disease

SSGCID Protocol List

Process Name Protocol
Text
Target selection Target Selection CIDR Batch20_Ver1_0
Target selection Target Selection CIDR Batch21_Ver1_0
Target selection Target Selection CIDR Batch22_Ver1_0
Target selection Target Selection CIDR Batch23_Ver1_0
Target selection Target Selection CIDR Batch24_Ver1_0
Target selection Target Selection CIDR Batch25_Ver1_0
Target selection Target Selection SBRI Batch01_Ver1_0
Target selection Target Selection SBRI Batch02_Ver1_0
Target selection Target Selection SBRI Batch03_Ver1_0
Target selection Target Selection SBRI Batch04_Ver1_0
Target selection Target Selection SBRI Batch05_Ver1_0
Target selection Target Selection SBRI Batch06_Ver1_0
Target selection Target Selection SBRI Batch08_Ver1_0
Target selection Target Selection SBRI CR_22
Target selection Target Selection SBRI CR_Ver1_0
Target selection Target SelectionSBRI Batch07_Ver1_0
Target selection Target SelectionSBRI Batch09_Ver1_0
Target selection Target SelectionSBRI Batch10_Ver1_0
Target selection Target SelectionSBRI Batch11_Ver1_0
Target selection Target SelectionSBRI Batch12_Ver1_0
Target selection Target SelectionSBRI Batch13_Ver1_0
Target selection Target SelectionSBRI Batch14_Ver1_0
Target selection Target SelectionSBRI Batch15_Ver1_0
Target selection Target SelectionSBRI Batch16_Ver1_0
Target selection Target SelectionSBRI Batch17_Ver1_0
Target selection Target SelectionSBRI Batch18_Ver1_0
Target selection Target SelectionSBRI Batch19_Ver1_0
Target selection Target SelectionSBRI Batch26_Ver1_0
Target selection Target SelectionSBRI Batch27_Ver1_0
Target selection Target SelectionSBRI Batch28_Ver1_0
Target selection Target SelectionSBRI Batch29_Ver1_0
Target selection Target SelectionSBRI Batch30_Ver1_0
Target selection Target SelectionSBRI Batch31_Ver1_0
Target selection Target SelectionSBRI Batch32_Ver1_0
Target selection Target SelectionSBRI Batch33_Ver1_0
Target selection Target SelectionSBRI Batch34_Ver1_0
Target selection Target SelectionSBRI Batch35_Ver1_0
Target selection Target SelectionSBRI Batch36_Ver1_0
Target selection Target SelectionSBRI Batch37_Ver1_0
Target selection Target SelectionSBRI Batch38_Ver1_0
Target selection Target SelectionSBRI Batch39_Ver1_0
PCR amplification
PCR amplification AE
PCR amplification CIDR: CloneAmp HIFI PreMix Ver1.0
PCR amplification CIDR: Gene/Fragment synthesis by IDT/Genewiz
PCR amplification CIDR: PCR Amplification with Phire Hot Start II Polymerase Master Mix
PCR amplification CIDR: PCR Amplification with Phire Hot Start II Polymerase Master Mix GC Rich
PCR amplification CIDR: Site Directed Mutagenesis
PCR amplification PCR Amplification Collaborator Ver 1.0
PCR amplification PCR Amplification Seattle Biomed pEU-INF Ver 1.0
PCR amplification PCR Amplification Seattle BioMed Phusion GC Ver. 1.0
PCR amplification PCR Amplification Seattle BioMed Phusion HF Ver. 1.0
PCR amplification PCR Amplification Seattle BioMed PrimeStar GC Ver. 1.0
PCR amplification PCR Amplification Seattle Biomed Ver 1.0
PCR amplification PCR Amplification UW PPG Gibson Ver 1.0
PCR amplification PCR amplification UW PPG Ver 1.0
PCR amplification PCR amplification UW PPG Ver 1.1
PCR amplification PCR amplification UW PPG Ver 1.2
PCR amplification PCR amplification UW PPG Ver 2.0
PCR amplification PCR amplification UW PPG Ver 2.1
PCR amplification PCR amplification UW PPG Ver 2.2
PCR amplification PCR amplification UW PPG Ver 3.0
PCR amplification PCR Amplification UW-CryoEM Ver 1.0
PCR amplification PCR Amplification with Platinum SuperFi Master Mix
PCR amplification PCR Amplification/RNA-Synthesis Collaborator Ver 1.0
PCR amplification PCR Emerald (iPCR for PIPE cloning)
Cloning CIDR pDuet-STII cloning Version 1.0
Cloning CIDR: Cloning InFusion Ver. 1.0
Cloning CIDR: Co-Expression Cloning via sequential-transformation Version 1.0
Cloning CIDR: Gibson Assembly for synthesized Gene Fragments
Cloning CIDR: NEBuilder HiFi DNA Assembly (tranformation via heatshock)
Cloning CIDR: NEBuilder HiFi DNA Assembly (transformation via electroporation)
Cloning CIDR: Restriction Enzyme Cloning Version 1.0
Cloning CIDR: Site Directed Mutagenesis-Cloning
Cloning cloned by collaborator
Cloning Cloning Collaborator Ver 1.0
Cloning Cloning Emerald PIPE Ver 1.0
Cloning Cloning Emerald RE Ver 2.0
Cloning Cloning Seattle BioMed InFusion pDuet Ver. 1.0
Cloning Cloning Seattle Biomed pEU-E01-MCS Ver 1.0
Cloning Cloning Seattle Biomed pEU-INF ver 1.0
Cloning Cloning UW PPG AVA Ver 1.0
Cloning Cloning UW PPG BG Ver 1.0
Cloning Cloning UW PPG Gibson Ver 1.0
Cloning Cloning UW PPG MBP Ver 1.0
Cloning Cloning UW PPG pMCSG26 Ver 1.0
Cloning Cloning UW RE Ver 1.0
Cloning Cloning UW-CryoEM Ver 1.0
Cloning Cloning UW-IPD Ver 1.0
Cloning Cloning/RNA-Synthesis Collaborator Ver 1.0
Cloning commercial vendor custom cloning
Sequence validation - clone Sequence Validation Emerald Ver 1.0
Expression testing AE expression
Expression testing CIDR: Expi293 Transfection
Expression testing CIDR: Small Scale Expression Testing for materials (Vector and insert) from Collaborator Ver 1.0
Expression testing CIDR: Small Scale Expression Testing for materials (Vector and insert) from Collaborator Ver 1.1
Expression testing CIDR: Small Scale Expression Testing for materials in SSGCID Vectors Ver 1.0
Expression testing CIDR: Small Scale Expression Testing with Takara Chaperone Plasmid Kit Ver.1.0
Expression testing Expression Sm Emerald Ver 1.0
Expression testing Expression Sm WEPRO 1240H and NV10 Seattle Biomed Ver 1.0
Expression testing Expression Sm WEPRO 1240H Seattle Biomed Ver 1.0
Expression testing Expression Sm WEPRO 7240H and NV10 Seattle Biomed Ver 1.0
Expression testing Expression Sm WEPRO 7240H Seattle Biomed Ver 1.0
Expression testing Expression Testing Collaborator Ver 1.0
Expression testing Expression Testing Seattle BioMed for Collaborator Ver 1.0
Expression testing Expression Testing Seattle BioMed Ver. 1.0
Expression testing Expression Testing UW-CryoEM Ver 1.0
Expression testing Expression Testing/RNA-Synthesis Collaborator Ver 1.0
Expression testing Expression UW PPG Ver 1.0
Expression testing Expression UW PPG Ver 1.1
Expression testing Expression UW PPG Ver 1.2
Expression testing Expression UW PPG Ver 1.4
Expression testing Noah Sather's HEK293 Expression testing protocol
Upscale Large Scale Expression UW PPG ver 3.0
Upscale CIDR: Expi293 Transfection Upscale
Upscale CIDR: Upscale (AUTO Induction) Version 1.0
Upscale CIDR: Upscale(IPTG Induction) Version 1.0
Upscale Expression Lg Emerald Ver 1.0
Upscale Large Scale Expression Seattle BioMed Ver. 1.0
Upscale Large Scale Expression UW PPG Ver 1.0
Upscale Large Scale Expression UW PPG Ver 1.1
Upscale Large Scale Expression UW PPG Ver 2.0
Upscale Large Scale Expression UW PPG Ver 2.1
Upscale Large Scale Expression UW PPG Ver 7.1
Upscale SBRI XE Large Scale Productions w/NV10
Upscale Upscale 13C/15N Ver 1.0
Upscale Upscale 15N @ PNNL
Upscale Upscale 15N Ver 1.0
Upscale Upscale Collaborator Ver 1.0
Upscale Upscale Seattle BioMed for Collaborator Ver 1.0
Upscale Upscale Seattle BioMed for Collaborator Ver 2.0
Upscale Upscale SeMet Ver 1.0
Upscale Upscale UW-CryoEM Ver 1.0
Upscale Upscale/RNA-Synthesis Collaborator Ver 1.0
Protein Purification CIDR: Bug Buster Lysis & Purification
Protein Purification CIDR: Tier 1 Purification
Protein Purification CIDR: Cation Exchange Purification of Collaborator Provided Construct Version 1.0
Protein Purification CIDR: Complex Purification IMAC-StrepTrepActin Ver. 1.0
Protein Purification CIDR: Complex Purification IMAC-StrepTrepActin-IMAC Ver. 1.0
Protein Purification CIDR: Hypotonic Lysis & Purification
Protein Purification CIDR: IMAC-Anion-Ulp-SEC
Protein Purification IMAC only
Protein Purification IMAC-Q HP-SEC
Protein Purification Protein L - Buffer exchange
Protein Purification Purification Collab Ver 1.0
Protein Purification Purification Emerald Ver 1.0
Protein Purification Purification PNNL Ver 1.0
Protein Purification Purification Seattle BioMed Complex IMAC-SEC Ver. 1.0
Protein Purification Purification Seattle BioMed Complex IMAC-StrepTrepActin Ver. 1.0
Protein Purification Purification Seattle BioMed Complex IMAC-StrepTrepActin-SEC Ver. 1.0
Protein Purification Purification Seattle BioMed for Collaborator Ver 1.0
Protein Purification Purification Seattle BioMed Ver 1.0
Protein Purification Purification UW NMR Ver 1.0
Protein Purification Purification UW PPG 3C Cleaved Ver 1.0
Protein Purification Purification UW PPG 3C Cleaved Ver 1.1
Protein Purification Purification UW PPG 3C Cleaved Ver 2.0
Protein Purification Purification UW PPG 3C Cleaved Ver 2.1
Protein Purification Purification UW PPG 3C Cleaved Ver 3.0
Protein Purification Purification UW PPG Amylose and SEC
Protein Purification Purification UW PPG uncleaved 3.0
Protein Purification Purification UW PPG Uncleaved Ver 1.0
Protein Purification Purification UW PPG Uncleaved Ver 1.1
Protein Purification Purification UW PPG Uncleaved Ver 2.0
Protein Purification Purification UW PPG Uncleaved Ver 4.0
Protein Purification Purification UW-CryoEM Ver 1.0
Protein Purification Purification UW-IPD HEK293 IMAC-SEC
Protein Purification Seattle BioMed Strepactin - SEC
Crystallization Beryllium Crystallization Protocol
Crystallization Crystallization Emerald Ver 1.0
Crystallization Crystallization partial enzymatic digest Ver 1.0
HSQC testing HSQC PNNL Ver 1.0
NMR data collection NMR collect assign PNNL Ver 1.0
Backbone assignment NMR assign PNNL Ver 1.0
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