NIAID Emerging and Re-emerging Infectious Diseases NIAID Bioinformatics Resource Centers NIAID Structural Genomic Centers Center for Structural Genomics of Infectious Disease
Seattle Structural Genomics Center for Infectious Disease

BaboA.01191.a: Dihydrofolate reductase/thymidylate synthase

Target Characteristics

from organism: Babesia bovis T2Bo
most recent status: in PDB
center reference id: BaboA.01191.a
is community request: True
associated disease: Babesiosis (atypical)
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BaboA.01191.a.D11.GE00003 BaboA.01191.a 1 511 GE00003
BaboA.01191.a.D12.GE00001 BaboA.01191.a 1 188 GE00001
BaboA.01191.a.D13.GE00001 BaboA.01191.a 203 511 GE00001
BaboA.01191.a.D14.GE00001 BaboA.01191.a 203 499 GE00001
BaboA.01191.a.D15.GE00001 BaboA.01191.a 225 511 GE00001
BaboA.01191.a.D16.GE00001 BaboA.01191.a 225 499 GE00001
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BaboA.01191.a.D13.PD00068 BaboA.01191.a 203 511

Structures

image of structure for 3I3R
3I3R
Deposited: 6/30/2009
Determination: XRay
Diffraction Image
Clone: BaboA.01191.a.D11.GE00003
Protein: BaboA.01191.a.D11.PD00043
image of structure for 3K2H
3K2H
Deposited: 9/30/2009
Determination: XRay
Diffraction Image
Clone: BaboA.01191.a.D11.GE00003
Protein: BaboA.01191.a.D11.PD00043
image of structure for 3KJR
3KJR
Deposited: 11/3/2009
Determination: XRay
Diffraction Image
Clone: BaboA.01191.a.D11.GE00003
Protein: BaboA.01191.a.D11.PD00043
image of structure for 3NRR
3NRR
Deposited: 6/30/2010
Determination: XRay
Diffraction Image
Clone: BaboA.01191.a.D11.GE00003
Protein: BaboA.01191.a.D11.PD00043

Publications by SSGCID

Inhibitor-bound complexes of dihydrofolate reductase-thymidylate synthase from Babesia bovis
Abendroth J, Begley DW, Edwards TE, Hartley RC, Lorimer DD, Myler PJ, Raymond AC, Sankaran B, Smith ER, Staker BL, Stewart LJ
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1070-7
PMID: 21904052; PMCID: PMC3169404
PDB: 3I3R, 3K2H, 3NRR

Citations by Others

Structure-Based Targeting of Orthologous Pathogen Proteins Accelerates Antiparasitic Drug Discovery
V Jain, A Sharma, G Singh, M Yogavel - ACS Infectious , 2017 - ACS Publications
cited by: 2 others
PDB: 3I3R
Multiparameter screening on slipchip used for nanoliter protein crystallization combining free interface diffusion and microbatch methods
L Li, W Du, RF Ismagilov - Journal of the American Chemical Society, 2009 - ACS Publications
cited by: 67 others
PDB: 3I3R, 3KJR
Targeting plant DIHYDROFOLATE REDUCTASE with antifolates and mechanisms for genetic resistance
MG Corral, J Haywood, LH Stehl, KA Stubbs- The Plant, 2018 - Wiley Online Library
cited by: 1 other
PDB: 3K2H
Global optimization-based inference of chemogenomic features from drug–target interactions
S Zu, T Chen, S Li - Bioinformatics, 2015 - Oxford Univ Press
PDB: 3K2H
Large-scale Mining for Similar Protein Binding Pockets: With RAPMAD Retrieval on the Fly Becomes Real
T Krotzky, C Grunwald, U Egerland… - Journal of chemical …, 2014 - ACS Publications
cited by: 4 others
PDB: 3K2H
Structural insights into the molecular basis of the ligand promiscuity
N Sturm, J D?saphy, RJ Quinn, D Rognan? - Journal of Chemical Information and Modeling, 2012 - ACS Publications
cited by: 26 others
PDB: 3K2H
Antifolate agents: a patent review (2006-2010)
DL Wright, AC Anderson - Expert opinion on therapeutic patents, 2011 - informahealthcare.com
cited by: 12 others
PDB: 3K2H, 3NRR
Are homology models sufficiently good for free-energy simulations?
S Genheden - Journal of chemical information and modeling, 2012 - ACS Publications
cited by: 3 others
PDB: 3KJR
Pharmacoinformatic Study on the Selective Inhibition of the Protozoan Dihydrofolate Reductase Enzymes
VK Sharma, S Abbat, PV Bharatam - Molecular Informatics, 2017 - Wiley Online Library
PDB: 3NRR
Human Dihydrofolate Reductase and Thymidylate Synthase form a complex in vitro and co-localize in normal and cancer cells
A Antosiewicz, A Jarmua, D Przybylska - Structure and , 2016 - Taylor & Francis
cited by: 2 others
PDB: 3NRR
Rare Sidechain Conformations in Proteins and DNA
BJ Hintze - 2015 - search.proquest.com
PDB: 3NRR

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
EuPathDB: PiroplasmaDB:BBOV_II000780
OrthoMCL: OG5_127385
RefSeq: XP_001609606.1
UniProt: A7ASX7

Enzyme & Pathway Information

Pathway Pathway ID EC Number
N10-formyl-tetrahydrofolate biosynthesis 1CMET2-PWY 1.5.1.3
superpathway of chorismate metabolism ALL-CHORISMATE-PWY 1.5.1.3
superpathway of tetrahydrofolate biosynthesis and salvage FOLSYN-PWY 1.5.1.3
folate transformations II PWY-3841 1.5.1.3
superpathway of tetrahydrofolate biosynthesis PWY-6612 1.5.1.3
tetrahydrofolate biosynthesis PWY-6614 1.5.1.3
N10-formyl-tetrahydrofolate biosynthesis 1CMET2-PWY 2.1.1.45
purine and pyrimidine metabolism P1-PWY 2.1.1.45
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) PWY0-166 2.1.1.45
folate transformations II PWY-3841 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis I PWY-7184 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis II PWY-7187 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis IV PWY-7198 2.1.1.45
pyrimidine deoxyribonucleosides salvage PWY-7199 2.1.1.45
superpathway of pyrimidine deoxyribonucleoside salvage PWY-7200 2.1.1.45
pyrimidine deoxyribonucleotides biosynthesis from CTP PWY-7210 2.1.1.45
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis PWY-7211 2.1.1.45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSNSYEGCGD LTIFVAVALN KVIGHKNQIP WPHITHDFRF LRNGTTYIPP EVLSKNPDIQ NVVIFGRKTY ESIPKASLPL KNRINVILSR TVKEVPGCLV YEDLSTAIRD LRANVPHNKI FILGGSFLYK EVLDNGLCDK IYLTRLNKEY PGDTYFPDIP DTFEITAISP TFSTDFVSYD FVIYERKDCK TVFPDPPFDQ LLMTGTDISV PKPKYVACPG VRIRNHEEFQ YLDILADVLS HGVLKPNRTG TDAYSKFGYQ MRFDLSRSFP LLTTKKVALR SIIEELLWFI KGSTNGNDLL AKNVRIWELN GRRDFLDKNG FTDREEHDLG PIYGFQWRHF GAEYLDMHAD YTGKGIDQLA EIINRIKTNP NDRRLIVCSW NVSDLKKMAL PPCHCFFQFY VSDNKLSCMM HQRSCDLGLG VPFNIASYSI LTAMVAQVCG LGLGEFVHNL ADAHIYVDHV DAVTTQIARI PHPFPRLRLN PDIRNIEDFT IDDIVVEDYV SHPPIPMAMS A

NT Sequence
atgtctaatt catacgaagg gtgtggagat cttacgattt tcgtagccgt tgccttgaat aaggtaattg gccataaaaa ccaaattccc tggccgcata ttacgcatga ttttcgtttt ttgcgtaatg gcacaactta cattccacct gaggtgttgt ctaagaaccc agatatacag aatgttgtga ttttcggtag gaagacttat gaaagtatcc ctaaagcttc gctccctctt aaaaaccgta taaacgtgat actgtctcgt actgtcaagg aagttcctgg ttgtttggtg tacgaggacc tttctactgc cattcgtgat ttgcgtgcaa atgtccctca taacaagatt tttatcttag gcggttcttt tctttacaag gaggtgcttg acaatggttt gtgtgataag atttatttga ctcgtctcaa caaggagtat cctggtgaca cctacttccc tgatattccg gatacttttg agataaccgc cattagtcct acgttttcca ctgactttgt cagttatgat tttgttattt acgagcgtaa ggattgcaag actgttttcc ccgatcctcc ttttgatcaa ttattgatga cagggactga cattagcgtt cccaaaccaa agtacgttgc ttgtcctggc gtcaggatac gcaaccatga agagttccaa taccttgaca tattggcaga tgtgctaagt catggtgtat tgaagcccaa ccgtaccggt actgatgcct actctaagtt tggctatcag atgcgatttg acctttcacg ttcattcccg ttattgacta ccaagaaggt agcattgcgc agcataattg aggaactgct ctggtttatt aagggtagta ccaacggcaa cgacctactg gctaagaatg tgcgcatttg ggagcttaac ggtcgtcgtg atttccttga taagaatggt tttacagatc gtgaagagca tgatttaggt cctatatatg gtttccaatg gcgtcatttt ggtgctgagt acttggatat gcacgctgat tacactggta agggtattga tcagcttgct gagataatta accgcattaa aacgaaccca aacgaccgta ggctgattgt atgctcttgg aatgtttcgg acctaaagaa gatggctttg cctccttgtc attgtttctt ccaattttat gtcagtgaca acaagctttc ttgtatgatg catcagcgtt cctgtgacct tggtttgggt gtacctttca acattgcttc ttatagtata cttactgcta tggtggctca ggtttgcggt ttaggtttag gggagtttgt ccataatttg gcagacgccc acatttatgt tgaccacgtg gacgccgtta ctactcagat tgctcgcatt cctcatccat ttccccgttt gcgtttgaac cctgatatta ggaatatcga ggactttact attgacgaca ttgtagttga ggattacgtc tcgcatcctc ccatacccat ggccatgtcc gcatga

© 2020 Seattle Children's Research Institute. All rights reserved.