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Seattle Structural Genomics Center for Infectious Disease

BrabA.00010.e: 7-alpha-hydroxysteroid dehydrogenase

Target Characteristics

from organism: Brucella melitensis biovar Abortus 2308
most recent status: in PDB
center reference id: BrabA.00010.e
is community request: False
associated disease: Brucellosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00010.e.A1.GE25556 Full length( BrabA.00010.e ) 1 255 GE25556
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

image of structure for 3GAF
3GAF
Deposited: 2/17/2009
Determination: XRay
Diffraction Image
Clone: BrabA.00010.e.A1.GE25556
Protein: BrabA.00010.e.A1.PW25601

Citations by Others

Carboxyl-Terminal and Arg38 are Essential for Activity of the 7α-Hydroxysteroid Dehydrogenase from Clostridium absonum
D Lou, B Wang, J Tan, L Zhu - Protein and peptide letters, 2014 - ingentaconnect.com
cited by: 1 other
PDB: 3GAF
Computational analysis of the SAG 13 gene encoding an alcohol dehydrogenase
M Adhikary, S Ganguli, HJ Chakraborty, P Basu et al. - Journal of Pharmaceutical and Biomedical Sciences - jpbms.info
PDB: 3GAF

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_129079
PATRIC ID: fig|359391.4.peg.1693
RefSeq: YP_414992.1
UniProt: Q2YQ97

Enzyme & Pathway Information

Pathway Pathway ID EC Number
glycocholate metabolism (bacteria) PWY-6518 1.1.1.159
ursodeoxycholate biosynthesis (bacteria) PWY-7588 1.1.1.159

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSYESPFHLN DAVAIVTGAA AGIGRAIAGT FAKAGASVVV TDLKSEGAEA VAAAIRQAGG KAIGLECNVT DEQHREAVIK AALDQFGKIT VLVNNAGGGG PKPFDMPMSD FEWAFKLNLF SLFRLSQLAA PHMQKAGGGA ILNISSMAGE NTNVRMASYG SSKAAVNHLT RNIAFDVGPM GIRVNAIAPG AIKTDALATV LTPEIERAML KHTPLGRLGE AQDIANAALF LCSPAAAWIS GQVLTVSGGG VQELD

NT Sequence
ATGTCCTATG AGAGCCCGTT CCACCTGAAT GATGCTGTTG CAATCGTCAC CGGAGCGGCG GCAGGTATCG GACGGGCCAT CGCCGGAACC TTCGCCAAGG CTGGTGCTTC GGTTGTCGTA ACCGACCTTA AAAGCGAAGG CGCTGAAGCC GTTGCAGCCG CAATAAGGCA AGCTGGCGGC AAGGCCATCG GGCTTGAATG CAACGTAACC GACGAGCAGC ATCGCGAAGC CGTCATCAAA GCGGCCCTCG ATCAGTTCGG CAAAATCACT GTTCTGGTCA ACAATGCGGG TGGAGGCGGC CCCAAGCCCT TCGATATGCC GATGAGCGAC TTTGAATGGG CCTTCAAGCT CAATCTCTTT TCCCTGTTTC GCCTGTCGCA ATTGGCTGCC CCACACATGC AGAAGGCTGG CGGCGGCGCA ATTCTCAATA TTTCGTCCAT GGCCGGAGAA AATACCAATG TCCGGATGGC CTCCTATGGT TCGTCCAAGG CGGCGGTCAA TCACCTCACC CGCAACATTG CCTTCGATGT CGGCCCAATG GGCATTCGCG TGAATGCCAT CGCACCCGGC GCGATCAAGA CCGATGCGCT CGCAACCGTT CTGACGCCTG AAATCGAACG CGCCATGCTC AAGCACACGC CGCTTGGCCG CCTCGGCGAA GCGCAGGATA TCGCCAATGC GGCGCTGTTC CTCTGTTCGC CCGCCGCAGC ATGGATCAGC GGTCAGGTTC TCACGGTTTC CGGCGGCGGC GTGCAGGAAC TCGAC

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