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Seattle Structural Genomics Center for Infectious Disease

BrabA.11339.a: Beta-lactamase-like

Target Characteristics

from organism: Brucella melitensis biovar Abortus 2308
most recent status: in PDB
center reference id: BrabA.11339.a
is community request: True
associated disease: Brucellosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.11339.a.A1.GE27785 Full length( BrabA.11339.a ) 1 272 GE27785
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.11339.a.A1.PW27673 Full length( BrabA.11339.a ) 1 272

Structures

image of structure for 3MD7
3MD7
Deposited: 3/30/2010
Determination: XRay
Diffraction Image
Clone: BrabA.11339.a.A1.GE27785
Protein: BrabA.11339.a.A1.PW27673
image of structure for 3PY5
3PY5
Deposited: 12/11/2010
Determination: XRay
Diffraction Image
Clone: BrabA.11339.a.A1.GE27785
Protein: BrabA.11339.a.A1.PW27673
image of structure for 3PY6
3PY6
Deposited: 12/11/2010
Determination: XRay
Diffraction Image
Clone: BrabA.11339.a.A1.GE27785
Protein: BrabA.11339.a.A1.PW27673
image of structure for 3QH8
3QH8
Deposited: 1/25/2011
Determination: XRay
Diffraction Image
Clone: BrabA.11339.a.A1.GE27785
Protein: BrabA.11339.a.A1.PW27673

Publications by SSGCID

BrabA.11339.a: anomalous diffraction and ligand binding guide towards the elucidation of the function of a 'putative beta-lactamase-like protein' from Brucella melitensis.
Abendroth J, Bhandari J, Dieterich S, Edwards TE, Gardberg AS, Myler PJ, Napuli AJ, Sankaran B, Staker BL, Stewart LJ, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1106-12
PMID: 21904058; PMCID: PMC3169410
PDB: 3MD7, 3PY5, 3PY6, 3QH8

Citations by Others

Rare Sidechain Conformations in Proteins and DNA
BJ Hintze - 2015 - search.proquest.com
PDB: 3MD7
Dual specificity and novel structural folding of yeast phosphodiesterase-1 for hydrolysis of second messengers cAMP and cGMP
Y Tian, W Cui, M Huang, H Robinson, Y Wan - Biochemistry, 2014 - ACS Publications
cited by: 1 other
PDB: 3MD7
The electrostatic profile of consecutive Cβ atoms applied to protein structure quality assessment
S Chakraborty, R Venkatramani, BJ Rao… - …, 2013 - ncbi.nlm.nih.gov
cited by: 2 others
PDB: 3MD7
Structure and mechanism of PhnP, a phosphodiesterase of the carbon-phosphorus lyase pathway
SM He, M Wathier, K Podzelinska, M Wong? - Biochemistry, 2011 - ACS Publications
cited by: 10 others
PDB: 3MD7
The UlaG protein family defines novel structural and functional motifs grafted on an ancient RNase fold
F Fernandez, F Garces, M L?pez-Estepa? - BMC evolutionary biology, 2011 - biomedcentral.com
cited by: 2 others
PDB: 3MD7
An unusual diphosphatase from the PhnP family cleaves reactive FAD photoproducts
GAW Beaudoin, Q Li, SD Bruner, AD Hanson- Biochemical Journal, 2018 - biochemj.org
PDB: 3PY5, 3PY6, 3QH8
Computational analysis of N-H...PI interactions and its impact on the structural stability of beta-lactamases
P Lavanya, S Ramaiah, A Anbarasu - Computers in Biology and Medicine, 2014 - Elsevier
cited by: 6 others
PDB: 3PY6
Influence of CH... O interactions on the structural stability of beta-lactamases
P Lavanya, S Ramaiah, A Anbarasu - Journal of Biological Physics, 2013 - Springer
cited by: 2 others
PDB: 3PY6
Cation-PI Interactions in beta-Lactamases: The Role in Structural Stability
P Lavanya, S Ramaiah, A Anbarasu - Cell biochemistry and biophysics, 2012 - Springer
PDB: 3PY6
Protein surface characterization using an invariant descriptor
ZA Deeb, DA Adjeroh, BH Jiang - Journal of Biomedical Imaging, 2011 - dl.acm.org
cited by: 4 others
PDB: 3QH8

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_129512
PATRIC ID: fig|359391.4.peg.1061
RefSeq: YP_414422.1
UniProt: Q2YQ74

Enzyme & Pathway Information

Pathway Pathway ID EC Number
methylphosphonate degradation I PWY0-1533 3.1.4.55
(aminomethyl)phosphonate degradation PWY-7805 3.1.4.55
glyphosate degradation III PWY-7807 3.1.4.55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MTSPRNCLRF TLLGCGSSPG VPRINGDWGK CDPKNPKNRR RRASLLVERY DAEGNNTVVV IDTGPDFRMQ MIDSGVHMLD AAVYTHPHAD HIHGIDDLRT YVVDNGRLMD VYANRLTRNR LYDTFGYCFE TPVGSSYPPI LSMHDIAPET PFSIEGAGGA IRFEPFSQVH GDIESLGFRI GSVVYCTDVS AFPEQSLQYI KDADVLIIGA LQYRPHPSHF SLGEALEWIE KLSPKRAILT HMHVPLDYET VMRETPHHVE PGYDGLRFEV AV

NT Sequence
GTGACTTCTC CCCGCAATTG CCTGCGTTTC ACATTGCTTG GCTGCGGCTC GTCGCCGGGC GTGCCGCGCA TCAATGGCGA TTGGGGCAAG TGCGATCCGA AAAATCCGAA AAATCGCCGC CGCCGCGCTT CTCTGCTTGT GGAACGCTAT GATGCGGAGG GAAACAATAC GGTTGTCGTA ATCGACACCG GCCCGGATTT TCGCATGCAG ATGATTGATT CTGGCGTGCA TATGCTGGAT GCGGCGGTTT ACACCCATCC CCATGCCGAC CACATTCACG GTATTGATGA TCTGCGTACC TATGTGGTCG ATAATGGGCG CCTGATGGAT GTCTATGCCA ACCGGCTGAC GCGCAACCGT CTCTACGACA CATTCGGTTA TTGTTTTGAA ACGCCCGTCG GGTCGAGTTA CCCGCCGATC CTCTCGATGC ACGATATCGC GCCGGAAACG CCCTTTTCGA TTGAGGGCGC GGGCGGCGCC ATCCGCTTCG AGCCGTTCAG CCAGGTGCAT GGCGATATTG AATCGCTGGG GTTCCGTATC GGCAGCGTTG TCTATTGCAC GGATGTCAGC GCATTTCCCG AGCAAAGCCT GCAATATATA AAGGACGCGG ATGTTCTCAT CATCGGCGCC CTGCAATATC GTCCGCACCC AAGCCATTTT TCGCTTGGGG AAGCGCTGGA GTGGATCGAG AAACTATCGC CGAAGCGCGC TATTCTCACC CATATGCATG TACCGCTCGA TTATGAAACG GTGATGCGGG AAACGCCGCA TCACGTGGAG CCGGGCTATG ACGGCCTTCG CTTTGAAGTG GCCGTT

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