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Seattle Structural Genomics Center for Infectious Disease

BrmeA.00249.a: Thymidylate synthase (EC 2.1.1.45)

Target Characteristics

from organism: Brucella melitensis 16M
most recent status: in PDB
center reference id: BrmeA.00249.a
is community request: True
associated disease: Brucellosis
NIH risk group: 3
is select agent: False
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrmeA.00249.a.D11.GE00001 BrmeA.00249.a 1 264 GE00001
BrmeA.00249.a.D12.GE00001 BrmeA.00249.a 1 232 GE00001
BrmeA.00249.a.D13.GE00001 BrmeA.00249.a 1 237 GE00001
BrmeA.00249.a.D15.GE00001 BrmeA.00249.a 1 252 GE00001
BrmeA.00249.a.D16.GE00001 BrmeA.00249.a 1 255 GE00001
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrmeA.00249.a.D11.PD00069 BrmeA.00249.a 1 264
BrmeA.00249.a.D15.PD00070 BrmeA.00249.a 1 252
BrmeA.00249.a.D16.PD00071 BrmeA.00249.a 1 255

Structures

image of structure for 3IX6
3IX6
Deposited: 9/3/2009
Determination: XRay
Diffraction Image
Clone: BrmeA.00249.a.D11.GE1
Protein: BrmeA.00249.a.D11.PD00069

Citations by Others

Analysis of mutations leading to para-aminosalicylic acid resistance in Mycobacterium tuberculosis
B Pandey, S Grover, J Kaur, A Grover- Scientific reports, 2019 - nature.com
PDB: 3IX6

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_127385
PATRIC ID: fig|224914.11.peg.682
RefSeq: NP_539525.1
UniProt: P67042

Enzyme & Pathway Information

Pathway Pathway ID EC Number
N10-formyl-tetrahydrofolate biosynthesis 1CMET2-PWY 2.1.1.45
purine and pyrimidine metabolism P1-PWY 2.1.1.45
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) PWY0-166 2.1.1.45
folate transformations II PWY-3841 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis I PWY-7184 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis II PWY-7187 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis IV PWY-7198 2.1.1.45
pyrimidine deoxyribonucleosides salvage PWY-7199 2.1.1.45
superpathway of pyrimidine deoxyribonucleoside salvage PWY-7200 2.1.1.45
pyrimidine deoxyribonucleotides biosynthesis from CTP PWY-7210 2.1.1.45
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis PWY-7211 2.1.1.45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MRTYLDLLQH VLDHGVDRDD RTGTGTRSVF GYQMRFDLEE GFPVLTTKKL HLRSIIHELL WFLKGDTNIA YLKENGVTIW DEWADENGDL GPVYGYQWRS WPAPDGRHID QIANLLKMLH TNPQSRRLIV SAWNPALVDE MALPPCHCLF QFYVANGRLS CQLYQRSADI FLGVPFNIAS YALLTMMIAQ VTGLKPGEFI HTLGDAHIYS NHFEQARLQL TRTPKKLPVM HINPDVKDLF AFRFEDFRLD GYEADPTIKA PIAV

NT Sequence
atgcgcacct atcttgatct tctccagcat gtgctcgacc acggcgttga ccgtgacgac cgcacgggaa cgggcacacg ctcggtcttc ggctatcaga tgcggttcga cctggaggag gggtttccgg ttctcacgac caagaaactc catttgcgtt cgatcattca tgaactcttg tggttcctga aaggcgatac gaacattgcc tatctgaaag aaaacggcgt tacgatctgg gatgaatggg ccgatgaaaa cggcgatctg gggccggttt atggctatca gtggcgttca tggcctgcgc cggacggacg gcatatcgac cagatcgcga atcttttgaa gatgttgcac acaaatcctc agtcgcggcg tctgattgtt tcggcgtgga acccggcgct ggtggatgaa atggctctgc cgccctgcca ctgcctgttc cagttctatg tcgccaatgg caggctttcc tgccagcttt accagcgttc cgcggatatt ttcctcggcg tgccgttcaa tatcgcctcc tatgccctgt tgacgatgat gatcgcgcag gtgacggggc tgaaaccggg cgagttcatc catacgctgg gcgatgccca tatttattcc aaccatttcg agcaggcacg actacaactg acccgcacgc cgaagaagct gccggtgatg catatcaacc cggacgtgaa ggatctcttt gcctttcgtt tcgaggattt ccggctggat ggctatgaag ccgaccccac gatcaaagcg cctatcgcgg tatag

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