NIAID Emerging and Re-emerging Infectious Diseases NIAID Bioinformatics Resource Centers NIAID Structural Genomic Centers Center for Structural Genomics of Infectious Disease
Seattle Structural Genomics Center for Infectious Disease

BrmeA.18179.a: 2-isopropylmalate synthase (EC 2.3.3.13) (Alpha-IPM synthase) (Alpha-isopropylmalate synthase)

Target Characteristics

from organism: Brucella melitensis 16M
most recent status: expressed
center reference id: BrmeA.18179.a
is community request: True
associated disease: Brucellosis
NIH risk group: 3
is select agent: False
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrmeA.18179.a.A1.GE33732 full length 1 555 GE33732
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

Resource Reference ID
OrthoMCL: OG5_132150
PATRIC ID: fig|224914.11.peg.508
RefSeq: NP_539368.2
UniProt: Q8YIJ3

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of branched chain amino acid biosynthesis BRANCHED-CHAIN-AA-SYN-PWY 2.3.3.13
L-leucine biosynthesis LEUSYN-PWY 2.3.3.13
3-methylbutanol biosynthesis (engineered) PWY-6871 2.3.3.13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MPIAGTKYAP FPAPQLDDRT WPSKRIEKAP IWCSVDLRDG NQALIDPMGH DRKERMFRLL IDMGFPEIEI GFPSASQTDF DFCRWAIEQG DVPDDVDLQV LVQCRPELIT RTFEALEGAK TPIIHFYNST SELQRRVVFA KDVGGIKQIA TDAAKMIMDM AAKAGGGYRF QYSPESFTGT ELDVALEICN AVIEIVKPTP DNKLIVNLPS TVEMNTPNVY ADQIEWMCRN LDNRESLIIS LHPHNDRGTG IAATELGLMA GADRGEGTLF GNGERTGNVD VVTLALNMYT QGIDPGLDCT DINRMKEVYE YSNQLKIAER HPYVGELVYT AFSGSHQDAI NKGMKARRSA NSPVWEVPYL PIDPQDVGRS YEAIIRINSQ SGKGGIAYIL QADYGLNLPR NLQVEFREII QHITDEEGKE LPSKRIYEEF QKLYVTQPDA RIKFVDHHTY PHPEQKGRRI LTAEITDNGV TKTIEGKGTG PIDGFVDALS KYLGVKMSVV DYSEHSLQQG SDASAISYVE MVYPGGKLFG VGINDNIVSA SLEAVVSAAN RVIAK

NT Sequence
ttgaaagccg ggaggaaacg agaaatgaac cagcctgtct ccacgccaaa gtccgtgtcc caagccgtat cggcacgcaa gggcatgcct attgccggta ccaaatacgc gccgttccct gcgccgcagc tcgatgaccg cacttggcct tccaaacgca tcgaaaaggc gccgatctgg tgttcggtcg atctgcgcga cggcaaccag gctttgatcg accccatggg gcatgaccgc aaggagcgca tgttccgcct gctgatcgat atgggtttcc cggaaatcga gatcggtttc ccgtccgctt cgcagacgga tttcgacttc tgccgctggg ccattgaaca gggcgatgtt cctgacgacg tggatttgca ggttctggtc cagtgccgtc ctgaattgat tacgcgcact ttcgaggcgc tggaaggcgc gaagacgccg atcatccatt tctacaattc caccagcgaa ttgcagcgcc gcgtggtgtt tgccaaggat gtcggcggca tcaagcagat tgcaaccgat gcggccaaga tgatcatgga tatggctgcc aaggctggcg gcggttatcg cttccagtat tcgccggaaa gctttaccgg caccgaactg gatgtggcgc tggaaatctg caatgcggtg atcgagatcg tgaagccgac gccggacaac aagctgatcg tcaacctgcc ttcgacggtg gagatgaaca cgccgaatgt ctatgccgac cagatcgaat ggatgtgccg caatctggac aatcgcgaga gcctgattat ctcgctgcat ccgcataatg accgcggcac gggcattgcc gccactgaac tgggcctgat ggcaggcgct gatcgcgggg aaggcacgct gttcggcaat ggcgagcgca cgggcaatgt ggatgtggtg acgctggcgc tcaacatgta cacgcagggc atcgaccccg ggctggattg caccgatatc aaccggatga aggaagtcta cgaatattcc aaccagttga agatcgccga gcgccatcct tatgtgggcg agctggtcta tacggctttc tccggctcgc atcaggatgc gatcaacaag ggcatgaaag cgcgccgttc ggccaattcg ccggtttggg aagttcctta cctgccgatc gatccgcagg atgtgggccg ctcctacgag gcgatcatcc gcatcaattc gcaatcgggc aagggcggta tcgcctatat cctgcaagcc gattacggcc tgaacctgcc gcgcaacctg caggtcgagt tccgcgaaat catccagcac attacggatg aagagggcaa ggagctgcca tcgaagcgca tttacgagga gttccagaaa ctctatgtga cgcagcccga tgcccgcatc aagttcgtgg atcaccacac ctatccccat cctgagcaaa agggccggcg catcctgacg gctgagatca ccgataacgg tgtcaccaag actatcgagg gcaagggcac gggaccgatc gatggtttcg tggatgcgct gtcgaaatat cttggcgtga aaatgtcggt ggtcgattat tccgaacatt cgctccagca gggttcggat gcttcggcca tttcctatgt cgagatggtt tatcccggcg gcaagctgtt cggtgtcggc atcaacgata atatcgtcag cgcctcgctt gaagccgtgg tgtcggcggc aaatcgtgtg attgcgaagt ag

© 2019 Seattle Children's Research Institute. All rights reserved.