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Seattle Structural Genomics Center for Infectious Disease

BrsuA.18179.a: 2-isopropylmalate synthase (EC 2.3.3.13) (Alpha-IPM synthase) (Alpha-isopropylmalate synthase)

Target Characteristics

from organism: Brucella suis 1330
most recent status: purified
center reference id: BrsuA.18179.a
is community request: True
associated disease: Brucellosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrsuA.18179.a.A1.GE33968 full length 1 555 GE33968
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrsuA.18179.a.A1.PS01442 full length 1 555

External Resources

Resource Reference ID
OrthoMCL: OG5_132150
PATRIC ID: fig|204722.5.peg.1593
RefSeq: NP_698557.2
UniProt: Q8FZC4

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of branched chain amino acid biosynthesis BRANCHED-CHAIN-AA-SYN-PWY 2.3.3.13
L-leucine biosynthesis LEUSYN-PWY 2.3.3.13
3-methylbutanol biosynthesis (engineered) PWY-6871 2.3.3.13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MPIAGTKYAP FPAPQLDDRT WPSKRIEKAP IWCSVDLRDG NQALIDPMGH DRKERMFRLL IDMGFPEIEI GFPSASQTDF DFCRWAIEQG DVPDDVDLQV LVQCRPELIT RTFEALEGAK TPIIHFYNST SELQRRVVFA KDVGGIKQIA TDAAKMIMDM AAKAGGGYRF QYSPESFTGT ELDVALEICN AVIEIVKPTP DNKLIVNLPS TVEMNTPNVY ADQIEWMCRN LDNRESLIIS LHPHNDRGTG IAATELGLMA GADRVEGTLF GNGERTGNVD VVTLALNMYT QGIDPGLDCT DINRMKEVYE YSNQLKIAER HPYVGELVYT AFSGSHQDAI NKGMKARRSA NSPVWEVPYL PIDPQDVGRS YEAIIRINSQ SGKGGIAYIL QADYGLNLPR NLQVEFREII QHITDEEGKE LPSKRIYEEF QKLYVTQPDA RIKFVDHHTY PDPEQKGRRI LTAEITDNGV TKTIEGKGTG PIDGFVDALS KYLGVKMSVV DYSEHSLQQG SDASAISYVE MVYPGGKLFG VGINDNIVSA SLEAVVSAAN RVIAK

NT Sequence
atgaaccagc ctgtctccac gccaaagtcc gtgtcccaag ccgtatcggc acgcaagggc atgcctattg ccggtaccaa atacgcgccg ttccctgcgc cgcagctcga tgaccgcact tggccttcca aacgcatcga aaaggcgccg atctggtgtt cggtcgatct gcgcgacggc aaccaggctt tgatcgaccc catggggcat gaccgcaagg agcgcatgtt ccgcctgctg atcgatatgg gtttcccgga aatcgagatc ggtttcccgt ccgcttcgca gacggatttc gacttctgcc gctgggccat tgaacagggc gatgttcctg acgacgtgga tttgcaggtt ctggtccagt gccgtcctga attgattacg cgcactttcg aggcgctgga aggcgcgaag acgccgatca tccatttcta caattccacc agcgaattgc agcgccgcgt ggtgtttgcc aaggatgtcg gcggcatcaa gcagattgca accgatgcgg ccaagatgat catggatatg gctgccaagg ctggcggcgg ttatcgcttc cagtattcgc cggaaagctt taccggcacc gaactggatg tggcgctgga aatctgcaat gcggtgatcg agatcgtgaa gccgacgccg gacaacaagc tgatcgtcaa cctgccttcg acggtggaga tgaacacgcc gaatgtctat gccgaccaga tcgaatggat gtgccgcaat ctggacaatc gcgagagcct gattatctcg ctgcatccgc ataatgaccg cggcacgggc attgccgcca ctgaactggg cctgatggca ggcgctgatc gcgtggaagg cacgctgttc ggcaatggcg agcgcacggg caatgtggat gtggtgacgc tggcgctcaa catgtacacg cagggcatcg accccgggct ggattgcacc gatatcaacc ggatgaagga agtctacgaa tattccaacc agttgaagat cgccgagcgc catccttatg tgggcgagct ggtctatacg gctttctccg gctcgcatca ggatgcgatc aacaagggca tgaaagcgcg ccgttcggcc aattcgccgg tttgggaagt tccttacctg ccgatcgatc cgcaggatgt gggccgctcc tacgaggcga tcatccgcat caattcgcaa tcgggcaagg gcggtatcgc ctatatcctg caagccgatt acggcctgaa cctgccgcgc aacctgcagg tcgagttccg cgaaatcatc cagcacatta cggatgaaga gggcaaggag ctgccatcga agcgcattta cgaggagttc cagaaactct atgtgacgca gcccgatgcc cgcatcaagt tcgtggatca ccacacctat cccgatcctg agcaaaaggg ccggcgcatc ctgacggctg agatcaccga taacggtgtc accaagacta tcgagggcaa gggcacggga ccgatcgatg gtttcgtgga tgcgctgtcg aaatatcttg gcgtgaaaat gtcggtggtc gattattccg aacattcgct ccagcagggt tcggatgctt cggccatttc ctatgtcgag atggtttatc ccggcggcaa gctgttcggt gtcggcatca acgataatat cgtcagcgcc tcgcttgaag ccgtggtgtc ggcggcaaat cgtgtgattg cgaagtag

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