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Seattle Structural Genomics Center for Infectious Disease

BuceA.00102.a: 2-dehydro-3-deoxyphosphooctonate aldolase

Target Characteristics

from organism: Burkholderia cenocepacia J2315
most recent status: in PDB
center reference id: BuceA.00102.a
is community request: True
associated disease:
NIH risk group: 3
is select agent: False
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BuceA.00102.a.A1.GE32134 Full length( BuceA.00102.a ) 1 284 GE32134
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

image of structure for 3TML
3TML
Deposited: 8/31/2011
Determination: XRay
Diffraction Image
Clone: BuceA.00102.a.A1.GE32134
Protein: BuceA.00102.a.A1.PS01142

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

External Resources

Resource Reference ID
OrthoMCL: OG5_130461
PATRIC ID: fig|216591.5.peg.2269
RefSeq: YP_002231307.1
UniProt: B4EDA2

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of (KDO)2-lipid A biosynthesis KDO-NAGLIPASYN-PWY 2.5.1.55
superpathway of lipopolysaccharide biosynthesis LPSSYN-PWY 2.5.1.55
CMP-3-deoxy-D-manno-octulosonate biosynthesis PWY-1269 2.5.1.55
CMP-3-deoxy-D-manno-octulosonate biosynthesis II (from D-arabinose 5-phosphate) PWY-5111 2.5.1.55
CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis PWY-7674 2.5.1.55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MKLCDFEVGL DQPFFLIAGT CVVESEQMTI DTAGRLKEIC EKLNVPFIYK SSYDKANRSS GKSFRGLGMD EGLRILSEVK RQLGLPVLTD VHSIDEIEQV ASVVDVLQTP AFLCRQTDFI HACARSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLS EDRFMACERG VSFGYNNLVS DMRSLAIMRE TNAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAT GVAGLFMETH PNPAEAKSDG PNAVPLNRMG ALLETLVTLD QAVKRNPFLE NDFN

NT Sequence
ATGAAGCTGT GCGATTTCGA GGTCGGTCTC GACCAGCCTT TCTTCCTGAT CGCGGGTACC TGCGTCGTCG AATCGGAGCA AATGACGATC GACACGGCGG GCCGCCTGAA GGAGATCTGC GAGAAGCTGA ACGTTCCGTT CATCTACAAG TCGTCCTACG ACAAGGCAAA CCGCAGCTCG GGCAAGTCGT TTCGCGGCCT CGGAATGGAC GAGGGCCTGC GGATCCTGTC CGAGGTGAAG CGCCAGCTCG GCCTGCCGGT GCTGACCGAC GTGCATTCGA TCGACGAGAT CGAGCAGGTC GCGTCGGTCG TCGACGTGCT GCAGACGCCG GCGTTCCTGT GCCGCCAGAC CGACTTCATC CATGCGTGCG CGCGCTCGGG CAAGCCGGTC AACATCAAGA AGGGCCAGTT CCTCGCGCCG CACGACATGA AGAACGTGAT CGACAAGGCG CGCGACGCCG CACGTGAAGC CGGTCTGTCG GAAGACCGTT TCATGGCGTG CGAGCGCGGC GTCTCGTTCG GCTACAACAA TCTCGTGTCG GACATGCGTT CGCTCGCGAT CATGCGCGAG ACGAACGCGC CGGTCGTGTT CGATGCGACC CACTCGGTGC AATTGCCGGG CGGGCAGGGC ACGAGCTCGG GCGGCCAACG CGAATTCGTG CCGGTGCTCG CCCGTGCGGC GGTCGCGACG GGCGTCGCGG GCCTCTTCAT GGAAACGCAT CCGAATCCGG CCGAGGCGAA GTCGGACGGC CCGAATGCGG TGCCGCTGAA CCGGATGGGC GCGTTGCTGG AGACCCTCGT CACGCTCGAC CAGGCGGTGA AGCGCAATCC GTTCCTGGAA AACGATTTCA AT

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