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BupsA.00005.a: Malate dehydrogenase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00005.a
is community request: False
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00005.a.A1.GE24983 Full length( BupsA.00005.a ) 1 327 GE24983
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00005.a.A1.PW25021 Full length( BupsA.00005.a ) 1 327

Structures

image of structure for 3D5T
3D5T
Deposited: 5/16/2008
Determination: XRay
Diffraction Image
Clone: BupsA.00005.a.A1.GE24983
Protein: BupsA.00005.a.A1.PW25075

Citations by Others

Structures of citrate synthase and malate dehydrogenase of Mycobacterium tuberculosis
DM Ferraris, R Spallek, W Oehlmann… - Proteins: Structure, …, 2015 - Wiley Online Library
cited by: 5 others
PDB: 3D5T
Protein cold adaptation: Role of physico-chemical parameters in adaptation of proteins to low temperatures
S Shokrollahzade, F Sharifi, A Vaseghi… - Journal of theoretical …, 2015 - Elsevier
cited by: 1 other
PDB: 3D5T
'Cold spots' in protein cold adaptation: Insights from normalized atomic displacement parameters (< i> B'-factors)
A Siglioccolo, R Gerace, S Pascarella - Biophysical chemistry, 2010 - Elsevier
cited by: 13 others
PDB: 3D5T

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_127410
PATRIC ID: fig|320372.6.peg.4835
RefSeq: YP_335954
UniProt: Q3JKE9

Enzyme & Pathway Information

Pathway Pathway ID EC Number
respiration (anaerobic) ANARESP1-PWY 1.1.1.37
mixed acid fermentation FERMENTATION-PWY 1.1.1.37
gluconeogenesis I GLUCONEO-PWY 1.1.1.37
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass GLYCOLYSIS-TCA-GLYOX-BYPASS 1.1.1.37
glyoxylate cycle GLYOXYLATE-BYPASS 1.1.1.37
L-aspartate degradation II MALATE-ASPARTATE-SHUTTLE-PWY 1.1.1.37
TCA cycle IV (2-oxoglutarate decarboxylase) P105-PWY 1.1.1.37
pyruvate fermentation to propanoate I P108-PWY 1.1.1.37
reductive TCA cycle I P23-PWY 1.1.1.37
incomplete reductive TCA cycle P42-PWY 1.1.1.37
formaldehyde assimilation I (serine pathway) PWY-1622 1.1.1.37
L-carnitine degradation III PWY-3641 1.1.1.37
L-glutamate degradation VIII (to propanoate) PWY-5088 1.1.1.37
reductive TCA cycle II PWY-5392 1.1.1.37
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle PWY-5464 1.1.1.37
superpathway of glyoxylate cycle and fatty acid degradation PWY-561 1.1.1.37
TCA cycle II (plants and fungi) PWY-5690 1.1.1.37
partial TCA cycle (obligate autotrophs) PWY-5913 1.1.1.37
Methanobacterium thermoautotrophicum biosynthetic metabolism PWY-6146 1.1.1.37
TCA cycle III (animals) PWY66-398 1.1.1.37
gluconeogenesis III PWY66-399 1.1.1.37
methylaspartate cycle PWY-6728 1.1.1.37
TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase) PWY-6969 1.1.1.37
malate-oxaloacetate shuttle I PWY-7047 1.1.1.37
malate-oxaloacetate shuttle II PWY-7048 1.1.1.37
C4 photosynthetic carbon assimilation cycle, NAD-ME type PWY-7115 1.1.1.37
chitin degradation to ethanol PWY-7118 1.1.1.37
anaerobic energy metabolism (invertebrates, cytosol) PWY-7383 1.1.1.37
anaerobic energy metabolism (invertebrates, mitochondrial) PWY-7384 1.1.1.37
superpathway of anaerobic energy metabolism (invertebrates) PWY-7389 1.1.1.37
TCA cycle I (prokaryotic) TCA 1.1.1.37
superpathway of glyoxylate bypass and TCA TCA-GLYOX-BYPASS 1.1.1.37

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MAKPAKRVAV TGAAGQIAYS LLFRIANGDL LGKDQPVILQ LLDLPQAQAA VKGVVMELDD CAFPLLAGVV ITDDPKVAFK DADVALLVGA RPRSKGMERK DLLSANAEIF TVQGAALNEV ASRDVKVLVV GNPANTNAYI AMKSAPDLPK KNFTAMLRLD HNRALSQLAA KSGKPVASIE KLAVWGNHSP TMYPDFRFAT AEGESLLKLI NDDVWNRDTF IPTVGKRGAA IIEARGLSSA ASAANAAIDH VRDWVLGTNG KWVTMGIPSD GSYGIPEDII YGVPVICENG EYKRVEGLEI DAFSREKMDG TLAELLEERD GVAHLLK

NT Sequence
ATGGCTAAGC CCGCAAAGCG CGTTGCCGTC ACCGGCGCCG CAGGTCAGAT CGCTTACTCG CTGCTGTTTC GCATCGCGAA CGGCGACCTG CTCGGCAAGG ATCAGCCGGT CATCCTGCAA CTGCTCGACC TCCCGCAAGC CCAGGCCGCC GTCAAAGGCG TCGTGATGGA ACTGGACGAT TGCGCGTTCC CGCTGCTCGC GGGCGTCGTG ATCACCGACG ACCCGAAGGT CGCGTTCAAG GACGCCGACG TCGCGCTGCT GGTGGGCGCG CGTCCGCGCT CGAAGGGCAT GGAGCGCAAG GATCTGCTGT CGGCGAACGC CGAGATCTTC ACGGTCCAGG GCGCGGCGCT GAACGAAGTC GCGAGCCGCG ACGTGAAGGT GCTGGTCGTC GGCAACCCGG CGAACACGAA CGCGTACATC GCGATGAAGT CGGCGCCGGA TCTGCCGAAG AAGAACTTCA CGGCGATGCT GCGCCTCGAC CACAACCGCG CGCTGTCGCA GCTCGCCGCG AAGTCGGGCA AGCCGGTCGC GTCGATCGAG AAGCTCGCCG TGTGGGGCAA CCACTCGCCG ACGATGTACC CCGACTTCCG CTTCGCGACC GCCGAGGGCG AATCGCTGCT GAAGCTGATC AACGACGACG TGTGGAACCG CGACACGTTC ATCCCGACTG TCGGCAAGCG CGGCGCGGCG ATCATCGAAG CGCGCGGCCT GTCGTCGGCG GCGTCGGCGG CCAACGCGGC GATCGACCAC GTGCGCGACT GGGTGCTCGG CACGAACGGC AAGTGGGTCA CGATGGGCAT CCCGTCGGAC GGCTCGTACG GCATCCCCGA GGACATCATC TACGGCGTGC CGGTGATTTG CGAAAACGGC GAGTACAAGC GCGTCGAGGG CCTGGAAATC GACGCGTTCT CGCGCGAGAA GATGGACGGC ACGCTCGCCG AGCTGCTCGA GGAGCGCGAC GGCGTCGCCC ATCTGCTGAA G

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