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Seattle Structural Genomics Center for Infectious Disease

BupsA.00010.a: 3-ketoacyl-(acyl-carrier-protein) reductase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00010.a
is community request: True
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00010.a.A1.GE24986 Full length( BupsA.00010.a ) 1 249 GE24986
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00010.a.B1.PS00013 Full length( BupsA.00010.a ) 1 249

Structures

image of structure for 3FTP
3FTP
Deposited: 1/13/2009
Determination: XRay
Diffraction Image
Clone:
Protein: BupsA.00010.a.A1.PS0011

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

Citations by Others

Crystallographic analysis and structure-guided engineering of NADPH-dependent Ralstonia sp. alcohol dehydrogenase toward NADH cosubstrate specificity
A Lerchner, A Jarasch, W Meining? - Biotechnology and Bioengineering, 2013 - Wiley Online Library
PDB: 3FTP
Discovery of an L-Fucono-1, 5-Lactonase from cog3618 of the Amidohydrolase Superfamily
ME Hobbs, MW Vetting, HJ Williams? - Biochemistry, 2013 - ACS Publications
cited by: 6 others
PDB: 3FTP
Crystal structure of FabG4 from< i> Mycobacterium tuberculosis reveals the importance of C-terminal residues in ketoreductase activity
D Dutta, S Bhattacharyya, S Mukherjee, B Saha? - Journal of Structural Biology, 2011 - Elsevier
cited by: 10 others
PDB: 3FTP

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_126618
PATRIC ID: fig|320372.6.peg.3036
RefSeq: YP_334286
UniProt: Q3JQ67

Enzyme & Pathway Information

Pathway Pathway ID EC Number
biotin biosynthesis I BIOTIN-BIOSYNTHESIS-PWY 1.1.1.100
(5Z)-dodecenoate biosynthesis I PWY0-862 1.1.1.100
superpathway of fatty acid biosynthesis I (E. coli) PWY0-881 1.1.1.100
arachidonate biosynthesis PWY-5353 1.1.1.100
petroselinate biosynthesis PWY-5367 1.1.1.100
cis-vaccenate biosynthesis PWY-5973 1.1.1.100
superpathway of mycolate biosynthesis PWY-6113 1.1.1.100
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) PWY-6282 1.1.1.100
superpathway of unsaturated fatty acids biosynthesis (E. coli) PWY-6284 1.1.1.100
superpathway of fatty acids biosynthesis (E. coli) PWY-6285 1.1.1.100
8-amino-7-oxononanoate biosynthesis I PWY-6519 1.1.1.100
eicosapentaenoate biosynthesis from arachidonate PWY-6940 1.1.1.100
docosahexanoate biosynthesis II PWY-6951 1.1.1.100
docosahexanoate biosynthesis I PWY-7053 1.1.1.100
gondoate biosynthesis (anaerobic) PWY-7663 1.1.1.100
oleate biosynthesis IV (anaerobic) PWY-7664 1.1.1.100
(5Z)-dodecenoate biosynthesis II PWY-7858 1.1.1.100
mycolate biosynthesis PWYG-321 1.1.1.100

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MDKTLDKQVA IVTGASRGIG RAIALELARR GAMVIGTATT EAGAEGIGAA FKQAGLEGRG AVLNVNDATA VDALVESTLK EFGALNVLVN NAGITQDQLA MRMKDDEWDA VIDTNLKAVF RLSRAVLRPM MKARGGRIVN ITSVVGSAGN PGQVNYAAAK AGVAGMTRAL AREIGSRGIT VNCVAPGFID TDMTKGLPQE QQTALKTQIP LGRLGSPEDI AHAVAFLASP QAGYITGTTL HVNGGMFMS

NT Sequence
ATGGACAAGA CTCTCGACAA ACAAGTCGCG ATCGTCACCG GCGCGTCGCG CGGGATCGGC CGCGCGATCG CGCTCGAACT CGCGCGCCGC GGCGCAATGG TGATCGGCAC CGCGACGACC GAAGCGGGCG CCGAGGGCAT CGGCGCCGCG TTCAAGCAAG CCGGGCTCGA AGGGCGCGGC GCCGTGCTGA ACGTCAATGA CGCAACGGCG GTCGATGCGC TCGTCGAATC GACGCTCAAG GAGTTCGGCG CGCTGAACGT GCTCGTCAAC AATGCCGGCA TCACGCAGGA TCAGCTCGCG ATGCGGATGA AGGACGACGA GTGGGACGCC GTGATCGACA CGAATCTGAA GGCGGTGTTC CGTCTGTCGC GCGCGGTGCT GCGCCCGATG ATGAAGGCGC GCGGCGGACG CATCGTCAAT ATCACGTCGG TCGTCGGCTC CGCGGGCAAT CCGGGTCAGG TCAACTACGC GGCGGCGAAG GCCGGCGTCG CGGGAATGAC GCGCGCGCTC GCGCGCGAAA TCGGCAGCCG CGGCATCACC GTGAACTGCG TCGCGCCGGG CTTCATCGAT ACCGACATGA CGAAGGGCCT GCCGCAAGAG CAGCAGACGG CGCTGAAGAC CCAGATTCCG CTCGGCCGCC TGGGCAGCCC GGAGGACATC GCGCACGCGG TGGCGTTCCT CGCGTCGCCG CAGGCGGGCT ACATCACGGG CACGACGCTG CACGTGAACG GCGGCATGTT CATGTCG

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