NIAID Emerging and Re-emerging Infectious Diseases NIAID Bioinformatics Resource Centers NIAID Structural Genomic Centers Center for Structural Genomics of Infectious Disease
Seattle Structural Genomics Center for Infectious Disease

BupsA.00010.g: acetoacetyl-CoA reductase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00010.g
is community request: True
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00010.g.A1.GE25426 Full length( BupsA.00010.g ) 1 248 GE25426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

image of structure for 3GK3
3GK3
Deposited: 3/9/2009
Determination: XRay
Diffraction Image
Clone: BupsA.00010.g.A1.GE25426
Protein: BupsA.00010.g.A1.PS00076
image of structure for 5VT6
5VT6
Deposited: 5/15/2017
Determination: XRay
Diffraction Image
Clone: BupsA.00010.g.A1.GE25426
Protein: BupsA.00010.g.A1.PS00076

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

External Resources

Resource Reference ID
OrthoMCL: OG5_129142
PATRIC ID: fig|320372.6.peg.5074
RefSeq: YP_336172.1
UniProt: Q3JJT1

Enzyme & Pathway Information

Pathway Pathway ID EC Number
polyhydroxybutanoate biosynthesis PWY1-3 1.1.1.36
very long chain fatty acid biosynthesis PWY-5080 1.1.1.36
acetyl-CoA fermentation to butanoate II PWY-5676 1.1.1.36
ethylmalonyl-CoA pathway PWY-5741 1.1.1.36
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) PWY-7216 1.1.1.36
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) PWY-7218 1.1.1.36
4-oxopentanoate degradation PWY-7948 1.1.1.36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MQAKRVAFVT GGMGGLGAAI SRRLHDAGMA VAVSHSERND HVSTWLMHER DAGRDFKAYA VDVADFESCE RCAEKVLADF GKVDVLINNA GITRDATFMK MTKGDWDAVM RTDLDAMFNV TKQFIAGMVE RRFGRIVNIG SVNGSRGAFG QANYASAKAG IHGFTKTLAL ETAKRGITVN TVSPGYLATA MVEAVPQDVL EAKILPQIPV GRLGRPDEVA ALIAFLCSDD AGFVTGADLA INGGMHMS

NT Sequence
ATGCAAGCAA AACGCGTGGC ATTCGTGACG GGCGGTATGG GCGGACTGGG TGCGGCGATC AGCCGGCGGC TGCACGACGC CGGCATGGCT GTGGCGGTGT CGCATTCGGA GCGCAACGAT CATGTGTCGA CGTGGCTGAT GCACGAGCGC GACGCCGGAC GCGATTTCAA GGCGTATGCG GTCGACGTCG CGGATTTCGA ATCGTGCGAG CGGTGCGCGG AGAAGGTGCT CGCCGATTTC GGCAAGGTCG ACGTGCTGAT CAACAACGCG GGGATCACGC GCGATGCGAC GTTCATGAAG ATGACGAAGG GCGACTGGGA CGCGGTGATG CGCACCGATC TCGATGCGAT GTTCAACGTG ACGAAACAGT TCATCGCGGG CATGGTCGAG CGGCGCTTCG GGCGCATCGT CAACATCGGT TCGGTGAACG GCTCGCGCGG CGCGTTCGGT CAGGCGAATT ATGCGTCGGC GAAGGCGGGC ATCCACGGTT TCACGAAGAC GCTCGCGCTC GAGACGGCGA AGCGCGGGAT CACGGTCAAC ACGGTGTCGC CCGGCTATCT CGCGACCGCG ATGGTCGAAG CGGTGCCGCA GGACGTGCTC GAGGCGAAGA TCCTGCCGCA GATTCCGGTT GGCCGGCTCG GCCGGCCCGA CGAAGTGGCC GCGCTCATCG CGTTTCTGTG CTCGGACGAC GCGGGGTTCG TCACCGGCGC CGATCTCGCG ATCAACGGCG GGATGCACAT GTCC

© 2020 Seattle Children's Research Institute. All rights reserved.