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Seattle Structural Genomics Center for Infectious Disease

BupsA.00014.a: isocitrate lyase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00014.a
is community request: True
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00014.a.A1.GE24990 Full length( BupsA.00014.a ) 1 435 GE24990
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00014.a.D19.PD00020 Full length( BupsA.00014.a ) 1 435
BupsA.00014.a.A1.PW26982 Full length( BupsA.00014.a ) 1 435

Structures

image of structure for 3I4E
3I4E
Deposited: 7/1/2009
Determination: XRay
Diffraction Image
Clone: BupsA.00014.a.A1.GE24990
Protein: BupsA.00014.a.A1.PW25028

Citations by Others

Gluconeogenic precursor availability regulates flux through the glyoxylate shunt in Pseudomonas aeruginosa
A Crousilles, SK Dolan, P Brear, DY Chirgadze- Journal of Biological, 2018 - ASBMB
PDB: 3I4E
Potential Inhibitors for Isocitrate Lyase of Mycobacterium tuberculosis and Non-M. tuberculosis: A Summary
YV Lee, HA Wahab, YS Choong - BioMed Research International, 2015 - hindawi.com
cited by: 2 others
PDB: 3I4E
PEPTIDOMIMETIC COMPOUNDS
RT Skerlj, AC Good - US Patent 20,150,353,606, 2015 - freepatentsonline.com
PDB: 3I4E
Structure of the Vibrio cholerae fatty acid regulator FadR
W Shi - 2015 - search.proquest.com
PDB: 3I4E
Silencing Motifs in the Clr2 Protein from Fission Yeast, Schizosaccharomyces pombe
D Steinhauf, A Rodriguez, D Vlachakis, G Virgo… - PloS one, 2014 - dx.plos.org
PDB: 3I4E

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_129572
PATRIC ID: fig|320372.6.peg.2741
RefSeq: YP_334005
UniProt: Q3JQZ8

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass GLYCOLYSIS-TCA-GLYOX-BYPASS 4.1.3.1
glyoxylate cycle GLYOXYLATE-BYPASS 4.1.3.1
TCA cycle IV (2-oxoglutarate decarboxylase) P105-PWY 4.1.3.1
superpathway of glyoxylate cycle and fatty acid degradation PWY-561 4.1.3.1
TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase) PWY-6969 4.1.3.1
superpathway of glyoxylate bypass and TCA TCA-GLYOX-BYPASS 4.1.3.1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSRQQQAQEL QKQWETDPRW KGIKRAFTAE DVVRLRGSIQ QEHTLAKRGA EKLWTLINNE PFVNALGALT GNQAMQQVKA GLKAIYLSGW QVAGDANVAG EMYPDQSLYP ANSVPLVVKR INNTLTRADQ IQWSEGKNPG DEGYVDFFAP IVADAEAGFG GVLNAFELMK AMIEAGASGV HFEDQLASVK KCGHMGGKVL VPTREAVAKL TAARLAADVM GTPTVLVART DAEAADLITS DIDDNDKPYL TGERTVEGFF RTKPGLEQAI SRGLAYAPYA DLIWCETGKP DLEYAKKFAE AIHKQFPGKL LSYNCSPSFN WKKNLDDATI AKFQKELGAM GYKFQFITLA GFHALNYSMF NLAHGYARTQ MSAFVELQQA EFAAADKGFT AVKHQREVGT GYFDAVTQTV EREASTTALH GSTEDEQFFD GQKVA

NT Sequence
ATGTCGCGTC AACAACAGGC TCAGGAACTG CAAAAGCAAT GGGAAACCGA CCCGCGCTGG AAGGGCATCA AGCGCGCGTT CACCGCTGAG GACGTCGTGC GCCTGCGCGG CTCGATCCAG CAAGAGCACA CGCTCGCGAA GCGCGGCGCG GAAAAGCTCT GGACGCTCAT CAACAACGAG CCGTTCGTCA ACGCACTCGG CGCGCTGACG GGCAACCAGG CGATGCAGCA GGTCAAGGCC GGCCTCAAGG CGATCTATCT GTCGGGCTGG CAAGTCGCGG GCGACGCGAA CGTCGCGGGC GAAATGTATC CGGACCAGTC GCTGTACCCG GCGAACTCGG TGCCGCTCGT CGTCAAGCGC ATCAACAACA CGCTCACGCG CGCCGATCAG ATCCAGTGGT CGGAAGGCAA GAACCCCGGC GACGAAGGCT ATGTCGACTT CTTCGCGCCG ATCGTCGCCG ACGCGGAAGC CGGCTTCGGC GGCGTGCTGA ACGCGTTCGA ACTGATGAAG GCGATGATCG AGGCGGGCGC GTCGGGCGTT CACTTCGAAG ACCAGCTCGC CTCGGTCAAG AAGTGCGGCC ACATGGGCGG CAAGGTGCTC GTGCCGACCC GCGAGGCGGT CGCGAAGCTG ACGGCCGCGC GTCTCGCCGC CGACGTGATG GGCACGCCGA CCGTGCTGGT CGCGCGCACC GACGCCGAAG CGGCCGACCT GATCACGTCC GACATCGACG ACAACGACAA GCCGTACCTG ACGGGCGAGC GCACGGTCGA AGGCTTCTTC CGCACGAAGC CGGGCCTCGA GCAGGCTATC TCGCGCGGCC TCGCCTATGC GCCGTACGCC GACCTGATCT GGTGCGAAAC CGGCAAGCCG GATCTCGAAT ACGCGAAGAA GTTCGCGGAA GCGATCCACA AGCAGTTCCC GGGCAAGCTG CTGTCGTACA ACTGCTCGCC GTCGTTCAAC TGGAAGAAGA ACCTCGACGA CGCGACGATC GCGAAGTTCC AGAAGGAACT CGGCGCGATG GGCTACAAGT TCCAGTTCAT CACGCTCGCC GGCTTCCATG CGCTGAACTA CTCGATGTTC AACCTCGCGC ACGGCTACGC CCGCACGCAG ATGAGCGCGT TCGTCGAACT GCAGCAAGCC GAGTTCGCGG CGGCCGACAA GGGCTTCACC GCGGTCAAGC ACCAGCGTGA AGTCGGCACC GGCTACTTCG ACGCGGTGAC GCAGACGGTC GAGCGCGAAG CGTCGACGAC CGCGCTGCAC GGCTCGACCG AAGACGAACA GTTCTTCGAC GGCCAGAAAG TCGCC

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