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Seattle Structural Genomics Center for Infectious Disease

BupsA.00035.a: ribose-phosphate pyrophosphokinase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00035.a
is community request: True
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00035.a.A1.GE25009 Full length( BupsA.00035.a ) 1 318 GE25009
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00035.a.A1.PW25047 Full length( BupsA.00035.a ) 1 318

Structures

image of structure for 3DAH
3DAH
Deposited: 5/29/2008
Determination: XRay
Diffraction Image
Clone: BupsA.00035.a.A1.GE25009
Protein: BupsA.00035.a.A1.PW25047

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

Citations by Others

Crystal structure of E. coli PRPP synthetase
W Zhou, A Tsai, DA Dattmore- Structural, 2019 - bmcstructbiol.biomedcentral.com
PDB: 3DAH
Homo Multimer Protein Complexes
P Kangueane, C Nilofer- Protein-Protein and Domain-Domain Interactions, 2018 - Springer
PDB: 3DAH
Multiparameter screening on slipchip used for nanoliter protein crystallization combining free interface diffusion and microbatch methods
L Li, W Du, RF Ismagilov - Journal of the American Chemical Society, 2009 - ACS Publications
cited by: 67 others
PDB: 3DAH

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_126730
PATRIC ID: fig|320372.6.peg.763
RefSeq: YP_332167
UniProt: Q3JW86

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of histidine, purine, and pyrimidine biosynthesis PRPP-PWY 2.7.6.1
PRPP biosynthesis PWY0-662 2.7.6.1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis PWY-6404 2.7.6.1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSSHDGLMVF TGNANPALAQ EVVKILGIPL GKAMVSRFSD GEIQVEIQEN VRGKDVFVLQ STCAPTNDNL MELMIMVDAL KRASAGRITA AIPYFGYARQ DRRPRSARVA ISAKVVANML EIAGVERIIT MDLHADQIQG FFDIPVDNIY ATPILLGDLR KQNYPDLLVV SPDVGGVVRA RALAKQLNCD LAIIDKRRPK ANVAEVMNII GEVEGRTCVI MDDMVDTAGT LCKAAQVLKE RGAKQVFAYA THPVLSGGAA DRIAASALDE LVVTDTIPLS AESLACPKIR ALSSAGLLAE TFSRIRRGDS VMSLFAES

NT Sequence
ATGAGCAGCC ATGACGGCCT GATGGTTTTT ACTGGCAACG CCAATCCCGC GCTTGCTCAG GAAGTCGTCA AAATTCTGGG TATTCCCCTC GGCAAAGCAA TGGTCAGCCG TTTCTCCGAC GGCGAGATCC AAGTCGAAAT CCAGGAAAAC GTGCGCGGCA AGGACGTCTT CGTCCTGCAA TCGACGTGCG CGCCGACGAA CGACAACCTG ATGGAACTGA TGATCATGGT CGATGCGCTC AAGCGCGCAT CCGCCGGCCG GATCACCGCC GCCATCCCCT ACTTCGGCTA TGCGCGCCAG GATCGCCGCC CGCGTTCGGC GCGCGTCGCG ATCTCCGCGA AGGTCGTCGC GAACATGCTG GAAATCGCCG GCGTCGAGCG GATCATCACG ATGGATCTGC ACGCCGACCA GATTCAAGGC TTCTTCGACA TCCCCGTCGA CAACATCTAC GCGACGCCGA TCCTGCTGGG CGACCTGCGC AAGCAGAACT ACCCGGATCT GCTCGTCGTG TCGCCGGACG TCGGCGGCGT CGTGCGCGCC CGCGCGCTCG CGAAGCAGCT GAACTGCGAT CTCGCGATCA TCGACAAGCG CCGTCCGAAG GCGAATGTCG CCGAAGTGAT GAACATCATC GGTGAAGTCG AAGGCCGCAC CTGCGTGATC ATGGACGACA TGGTCGACAC GGCGGGCACG CTCTGCAAGG CCGCGCAAGT GCTCAAGGAG CGCGGCGCGA AGCAGGTGTT CGCGTATGCG ACGCACCCGG TGCTCTCGGG CGGCGCGGCC GACCGGATCG CCGCTTCGGC GCTCGACGAA CTTGTCGTCA CCGACACGAT TCCGCTGTCC GCCGAATCGC TCGCGTGCCC GAAGATCCGC GCGCTATCGA GCGCGGGCCT CCTCGCCGAG ACGTTCTCGC GGATCCGCCG CGGCGATTCG GTGATGTCGC TGTTCGCGGA ATCG

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