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Seattle Structural Genomics Center for Infectious Disease

BupsA.00040.a: S-adenosylmethionine synthetase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00040.a
is community request: True
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00040.a.A1.GE25014 Full length( BupsA.00040.a ) 1 395 GE25014
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00040.a.A1.PW25052 Full length( BupsA.00040.a ) 1 395

Structures

image of structure for 3IML
3IML
Deposited: 8/10/2009
Determination: XRay
Diffraction Image
Clone: BupsA.00040.a.A1.GE25014
Protein: BupsA.00040.a.A1.PW25052

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

Citations by Others

Structure of a thermostable methionine adenosyltransferase from Thermus thermophilus HB27 reveals a novel fold of the flexible loop
Y Liu, W Wang, W Zhang, Y Dong, F Han, M Raza - RSC Advances, 2016 - pubs.rsc.org
PDB: 3IML
Structure and function study of the complex that synthesizes S-adenosylmethionine
B Murray, SV Antonyuk, A Marina, SM Van Liempd - IUCrJ, 2014 - journals.iucr.org
PDB: 3IML
Structural and functional characterisation of the methionine adenosyltransferase from Thermococcus kodakarensis
J Schlesier, J Siegrist, S Gerhardt, A Erb? - BMC Structural Biology, 2013 - biomedcentral.com
PDB: 3IML
Identification and Characterization of the Chlamydia trachomatis L2 S-Adenosylmethionine Transporter
R Binet, RE Fernandez, DJ Fisher, AT Maurelli - mBio, 2011 - Am Soc Microbiol
cited by: 10 others
PDB: 3IML

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_126734
PATRIC ID: fig|320372.6.peg.397
RefSeq: YP_331809
UniProt: Q3JX94

Enzyme & Pathway Information

Pathway Pathway ID EC Number
ethylene biosynthesis I (plants) ETHYL-PWY 2.5.1.6
L-methionine degradation I (to L-homocysteine) METHIONINE-DEG1-PWY 2.5.1.6
superpathway of S-adenosyl-L-methionine biosynthesis MET-SAM-PWY 2.5.1.6
aspartate superpathway PWY0-781 2.5.1.6
S-adenosyl-L-methionine cycle II PWY-5041 2.5.1.6
superpathway of L-methionine salvage and degradation PWY-5328 2.5.1.6
2'-deoxymugineic acid phytosiderophore biosynthesis PWY-5912 2.5.1.6
S-adenosyl-L-methionine cycle I PWY-6151 2.5.1.6
superpathway of L-cysteine biosynthesis (mammalian) PWY-6292 2.5.1.6
L-methionine salvage cycle II (plants) PWY-7270 2.5.1.6
L-methionine salvage cycle III PWY-7527 2.5.1.6
L-methionine salvage cycle I (bacteria and plants) PWY-7528 2.5.1.6
L-cysteine biosynthesis VI (from L-methionine) PWY-I9 2.5.1.6
S-adenosyl-L-methionine biosynthesis SAM-PWY 2.5.1.6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MANDYLFTSE SVSEGHPDKV ADQISDAILD AILAQDKYSR VAAETLCNTG LVVLAGEITT TANIDYIQIA RDTIKRIGYD NTDYGIDYRG CAVLVAYDKQ SPDIAQGVDR AHDNNLDQGA GDQGLMFGYA CDETPELMPL PIHLSHRLVE RQANLRRDGR LPWLRPDAKS QVTVRYVDGK PHSIDTVVLS TQHAPEIDLP ALREAVIEEV IKPTLPADLI KGDIKFLVNP TGRFVIGGPQ GDCGLTGRKI IVDTYGGAAP HGGGAFSGKD PSKVDRSAAY AGRYVAKNIV AAGLASRALI QVSYAIGVAE PTSVMVNTFG TGRVSDETIT KLVREHFDLR PKGIIQMLDL LRPIYEKTAA YGHFGREEPE FSWEAADKAL ALAEAAGVEP AVQVA

NT Sequence
GTGGCAAACG ATTATCTCTT CACGTCCGAA TCCGTTTCCG AAGGCCATCC GGACAAGGTC GCCGACCAAA TCTCCGACGC GATCCTCGAC GCAATCCTCG CGCAAGACAA GTACTCCCGT GTCGCGGCCG AAACGCTGTG CAACACCGGT CTCGTCGTGC TCGCCGGCGA AATCACGACC ACGGCGAACA TCGACTACAT CCAGATCGCG CGCGACACGA TCAAGCGCAT CGGCTACGAC AATACCGATT ACGGCATCGA CTACCGCGGC TGCGCGGTGC TCGTCGCGTA CGACAAGCAG TCGCCGGACA TCGCGCAAGG CGTCGACCGC GCGCACGACA ACAACCTCGA TCAGGGCGCG GGCGATCAGG GCCTGATGTT CGGCTACGCG TGCGACGAAA CGCCCGAGCT GATGCCGCTG CCGATCCACC TGTCGCACCG GCTCGTCGAG CGCCAGGCGA ACCTGCGCCG CGACGGCCGC CTGCCGTGGC TGCGCCCGGA CGCGAAGTCA CAGGTGACGG TGCGCTACGT CGACGGCAAG CCGCACTCGA TCGACACCGT CGTGCTGTCG ACGCAGCACG CGCCGGAGAT CGATCTGCCC GCGCTGCGCG AAGCGGTGAT CGAAGAGGTC ATCAAGCCGA CGCTGCCCGC CGACCTGATC AAGGGCGACA TCAAGTTCCT CGTGAACCCG ACCGGCCGCT TCGTGATCGG CGGCCCGCAA GGCGATTGCG GGCTGACCGG CCGCAAGATC ATCGTCGACA CCTACGGCGG CGCGGCGCCG CACGGCGGCG GCGCGTTCTC GGGCAAGGAT CCGTCGAAGG TCGACCGCTC GGCCGCCTAC GCGGGCCGCT ACGTCGCGAA GAACATCGTC GCCGCGGGCC TCGCGTCGCG CGCGCTGATT CAGGTGTCGT ACGCGATCGG CGTGGCCGAG CCGACCTCGG TGATGGTCAA CACGTTCGGC ACGGGCCGCG TGTCCGACGA GACGATCACG AAGCTCGTGC GCGAGCACTT CGACCTGCGT CCGAAGGGCA TCATCCAGAT GCTCGACCTG CTGCGCCCGA TCTACGAGAA GACCGCCGCT TACGGCCACT TCGGCCGCGA AGAACCGGAG TTCTCGTGGG AAGCCGCCGA CAAGGCGCTC GCGCTCGCCG AAGCGGCGGG CGTCGAGCCG GCCGTGCAAG TGGCC

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