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Seattle Structural Genomics Center for Infectious Disease

BupsA.00092.a: isocitrate dehydrogenase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00092.a
is community request: True
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00092.a.A1.PW25020 Full length( BupsA.00092.a ) 1 419
BupsA.00092.a.A1.PW25104 Full length( BupsA.00092.a ) 1 419
BupsA.00092.a.B1.PW25188 Full length( BupsA.00092.a ) 1 419

Structures

image of structure for 3DMS
3DMS
Deposited: 7/1/2008
Determination: XRay
Diffraction Image
Clone: BupsA.00092.a.B1.GE30977
Protein: BupsA.00092.a.B1.PS00006

Publications by SSGCID

Structural basis of the substrate specificity of bifunctional isocitrate dehydrogenase kinase/phosphatase.
Bryan CM, Edwards TE, Jia Z, Myler PJ, Stein AJ, Stewart LJ, Van Voorhis WC, Yates SP, Zheng J
Biochemistry - 2011
volume 50, issue 38, pages 8103-6
PMID: 21870819; PMCID: PMC3354702
PDB: 3DMS

Citations by Others

Rare Sidechain Conformations in Proteins and DNA
BJ Hintze - 2015 - search.proquest.com
PDB: 3DMS
Induced Fit and the Catalytic Mechanism of Isocitrate Dehydrogenase
S Gon?alves, SP Miller, MA Carrondo, AM Dean? - Biochemistry, 2012 - ACS Publications
cited by: 12 others
PDB: 3DMS
Functional relevance of dynamic properties of Dimeric NADP-dependent Isocitrate Dehydrogenases
R Vinekar, C Verma, I Ghosh - BMC , 2012 - bmcbioinformatics.biomedcentral.
cited by: 7 others
PDB: 3DMS

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_126870
PATRIC ID: fig|320372.6.peg.1126
RefSeq: YP_332521
UniProt: Q3JV82

Enzyme & Pathway Information

Pathway Pathway ID EC Number
respiration (anaerobic) ANARESP1-PWY 1.1.1.42
mixed acid fermentation FERMENTATION-PWY 1.1.1.42
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass GLYCOLYSIS-TCA-GLYOX-BYPASS 1.1.1.42
TCA cycle IV (2-oxoglutarate decarboxylase) P105-PWY 1.1.1.42
reductive TCA cycle I P23-PWY 1.1.1.42
partial TCA cycle (obligate autotrophs) PWY-5913 1.1.1.42
L-glutamine biosynthesis III PWY-6549 1.1.1.42
methylaspartate cycle PWY-6728 1.1.1.42
TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase) PWY-6969 1.1.1.42
ethylene biosynthesis V (engineered) PWY-7124 1.1.1.42
superpathway NAD/NADP - NADH/NADPH interconversion (yeast) PWY-7245 1.1.1.42
TCA cycle VII (acetate-producers) PWY-7254 1.1.1.42
NAD/NADP-NADH/NADPH cytosolic interconversion (yeast) PWY-7268 1.1.1.42
TCA cycle VIII (helicobacter) REDCITCYC 1.1.1.42
TCA cycle I (prokaryotic) TCA 1.1.1.42
superpathway of glyoxylate bypass and TCA TCA-GLYOX-BYPASS 1.1.1.42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MPYQHIKVPE GGDKITVNKD FSLNVSDQPI IPYIEGDGTG FDITPVMIKV VDAAVEKAYG GKKKIHWMEI YAGEKATKVY GPDVWLPEET LQVLKEYVVS IKGPLTTPVG GGIRSLNVAL RQELDLYVCL RPIQYFKGVP SPVREPEKTN MVIFRENSED IYAGIEWAAE SEQAKKVIKF LQEEMGVKKI RFPQTSGIGI KPVSKEGTER LVRKAIQYAI DNDRKSVTLV HKGNIMKFTE GAFRDAGYAL AQKEFGAELI DGGPWMKFKN PKTGNEIVVK DSIADAFLQQ ILLRPAEYDV IATLNLNGDY ISDALAAQVG GIGIAPGANL SDSVAMFEAT HGTAPKYAGK DYVNPGSEIL SAEMMLRHLG WTEAADVIIS AMEKSIKQKR VTYDFARLME GATQVSCSGF GQVLIENME

NT Sequence
ATGCCGTATC AGCACATCAA GGTTCCGGAA GGCGGTGACA AGATCACCGT CAACAAGGAT TTCTCGCTCA ACGTTTCCGA TCAGCCGATC ATCCCTTACA TCGAGGGCGA CGGCACGGGC TTCGACATCA CGCCGGTGAT GATCAAGGTC GTCGACGCGG CGGTCGAGAA GGCGTACGGC GGCAAGAAGA AGATTCACTG GATGGAGATC TACGCCGGCG AGAAGGCGAC GAAGGTCTAC GGCCCGGACG TGTGGCTGCC GGAAGAGACG CTGCAGGTGC TGAAGGAATA CGTCGTGTCG ATCAAGGGGC CGCTCACGAC CCCGGTCGGC GGCGGCATCC GCTCGCTGAA CGTCGCGCTG CGTCAGGAGC TCGACCTGTA CGTCTGCCTG CGCCCGATCC AGTACTTCAA GGGCGTGCCT TCGCCCGTGC GCGAGCCGGA GAAGACCAAC ATGGTCATCT TCCGCGAGAA CTCGGAAGAC ATCTACGCCG GCATCGAATG GGCGGCCGAA TCCGAGCAGG CGAAGAAGGT CATCAAGTTC CTGCAGGAAG AGATGGGCGT GAAGAAGATC CGCTTCCCGC AGACCTCGGG CATCGGCATC AAGCCCGTGT CGAAGGAAGG CACCGAGCGC CTCGTGCGCA AGGCGATCCA GTACGCGATC GACAACGACC GCAAGTCGGT CACGCTCGTC CACAAGGGCA ACATCATGAA GTTCACGGAA GGTGCGTTCC GTGACGCCGG CTATGCGCTC GCGCAGAAGG AATTCGGCGC CGAGCTGATC GACGGCGGCC CGTGGATGAA GTTCAAGAAC CCGAAGACGG GCAACGAGAT CGTGGTCAAG GATTCGATCG CCGACGCGTT CCTGCAGCAG ATCCTGCTGC GCCCGGCCGA ATACGACGTG ATCGCGACGC TGAACCTGAA CGGCGACTAC ATCTCGGACG CGCTCGCCGC GCAAGTCGGC GGCATCGGCA TCGCGCCGGG CGCGAACCTG TCGGATTCGG TCGCGATGTT CGAAGCGACG CACGGCACGG CGCCGAAGTA CGCGGGCAAG GATTACGTGA ACCCGGGTTC GGAAATCCTG TCGGCCGAAA TGATGCTGCG CCACCTCGGC TGGACCGAAG CGGCCGACGT GATCATTTCC GCGATGGAGA AGTCGATCAA GCAGAAGCGC GTCACGTACG ACTTCGCGCG CCTGATGGAA GGCGCGACGC AAGTGTCGTG CTCGGGCTTC GGTCAGGTGC TGATCGAAAA CATGGAG

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