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Seattle Structural Genomics Center for Infectious Disease

BupsA.00102.a: 2-dehydro-3-deoxyphosphooctonate aldolase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00102.a
is community request: True
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00102.a.A1.GE28450 Full length( BupsA.00102.a ) 1 281 GE28450
BupsA.00102.a.A5.GE27947 Created from Decode construct for feature BupsA.00102.a 1 275 GE27947
BupsA.00102.a.A7.GE27949 Created from Decode construct for feature BupsA.00102.a 25 275 GE27949
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

image of structure for 3SZ8
3SZ8
Deposited: 7/18/2011
Determination: XRay
Diffraction Image
Clone: BupsA.00102.a.A1.GE28450
Protein: BupsA.00102.a.A1.PS00621
image of structure for 3TMQ
3TMQ
Deposited: 8/31/2011
Determination: XRay
Diffraction Image
Clone: BupsA.00102.a.A1.GE28450
Protein: BupsA.00102.a.A1.PS00621
image of structure for 3UND
3UND
Deposited: 11/15/2011
Determination: XRay
Diffraction Image
Clone: BupsA.00102.a.A1.GE28450
Protein: BupsA.00102.a.A1.PS00621

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

Citations by Others

Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa
SK Nelson, A Kelleher, G Robinson? - Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2013 - scripts.iucr.org
cited by: 3 others
PDB: 3UND

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_130461
PATRIC ID: fig|320372.6.peg.3401
RefSeq: YP_334635
UniProt: Q3JP68

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of (KDO)2-lipid A biosynthesis KDO-NAGLIPASYN-PWY 2.5.1.55
superpathway of lipopolysaccharide biosynthesis LPSSYN-PWY 2.5.1.55
CMP-3-deoxy-D-manno-octulosonate biosynthesis PWY-1269 2.5.1.55
CMP-3-deoxy-D-manno-octulosonate biosynthesis II (from D-arabinose 5-phosphate) PWY-5111 2.5.1.55
CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis PWY-7674 2.5.1.55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MNVAISPGVT AGNSLPFVLF GGINVLESLD FTLDVCGEYV AVTRKLGIPF VFKASFDKAN RSSIHSYRGV GLDEGLKIFA EVKARFGVPV ITDVHEAEQA APVAEIADVL QVPAFLARQT DLVVAIAKAG KPVNVKKPQF MSPTQLKHVV SKCGEVGNDR VMLCERGSSF GYDNLVVDML GFRQMAETTG GCPVIFDVTH SLQCRDPLGD ASGGRRRQVL DLARAGIAVG IAGLFLEAHP DPDRARCDGP SALPLHQLEG LLSQMKAIDD LVKRMPALEI R

NT Sequence
ATGAACGTAG CAATCAGCCC CGGCGTCACG GCCGGCAACA GCCTGCCTTT CGTGCTGTTC GGCGGGATCA ACGTGCTCGA GAGTCTCGAC TTCACGCTCG ACGTGTGCGG CGAATACGTC GCGGTGACGC GCAAGCTCGG CATTCCGTTC GTGTTCAAGG CGTCGTTCGA CAAGGCGAAC CGCTCGTCGA TCCATTCGTA TCGCGGCGTC GGGCTCGACG AAGGCCTGAA GATCTTCGCC GAGGTGAAGG CGCGCTTCGG CGTGCCGGTG ATCACCGATG TGCACGAAGC CGAGCAGGCG GCGCCCGTGG CCGAAATCGC CGACGTGTTG CAGGTGCCCG CGTTTCTCGC GCGGCAGACC GATCTCGTCG TCGCGATCGC GAAGGCCGGC AAGCCGGTGA ACGTGAAGAA GCCGCAGTTC ATGAGCCCCA CGCAATTGAA GCACGTGGTG TCGAAATGCG GCGAGGTCGG CAACGATCGC GTGATGCTGT GCGAGCGCGG CAGTTCGTTC GGCTACGACA ATCTCGTCGT GGACATGCTC GGCTTCCGGC AGATGGCCGA GACGACGGGC GGTTGCCCGG TGATCTTCGA CGTCACGCAC AGCCTGCAGT GCCGCGATCC GCTCGGCGAC GCGTCGGGCG GCCGGCGCCG GCAAGTGCTC GATCTCGCGC GCGCGGGCAT CGCGGTCGGC ATCGCGGGGC TCTTTCTCGA GGCGCACCCC GATCCCGACC GCGCGCGCTG CGATGGGCCG AGCGCGCTGC CGTTGCATCA GCTCGAGGGC TTGCTGTCGC AGATGAAGGC GATCGACGAT CTCGTCAAGC GCATGCCGGC GCTCGAGATT CGA

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