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Seattle Structural Genomics Center for Infectious Disease

BupsA.00130.a: peptidyl-prolyl cis-trans isomerase

Target Characteristics

from organism: Burkholderia pseudomallei 1710b
most recent status: in PDB
center reference id: BupsA.00130.a
is community request: True
associated disease: Melioidosis
NIH risk group: 3
is select agent: True
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00130.a.A1.GE25240 Full length( BupsA.00130.a ) 1 113 GE25240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00130.a.D228.PD00185 full length 2 113
BupsA.00130.a.D214.PD00190 full length 2 113
BupsA.00130.a.D219.PD00191 full length 2 113
BupsA.00130.a.D227.PD00193 full length 2 113
BupsA.00130.a.D24.PD00194 full length 2 113
BupsA.00130.a.D212.PD00195 full length 2 113
BupsA.00130.a.D242.PD00198 full length 2 113
BupsA.00130.a.D243.PD00199 full length 2 113
BupsA.00130.a.D248.PD00210 full length 2 113
BupsA.00130.a.D237.PD00212 full length 2 113
BupsA.00130.a.D240.PD00213 full length 2 113
BupsA.00130.a.A1.PS01414 Full length( BupsA.00130.a ) 1 113
BupsA.00130.a.A1.PS01451 Full length( BupsA.00130.a ) 1 113

Structures

image of structure for 2KE0
2KE0
Deposited: 1/21/2009
Determination: NMR
Clone: BupsA.00130.a.A1.GE25240
Protein: BupsA.00130.a.A1.PW25599
image of structure for 2KO7
2KO7
Deposited: 9/11/2009
Determination: NMR
Clone: BupsA.00130.a.A1.GE25240
Protein: BupsA.00130.a.A1.PW25599
image of structure for 2L2S
2L2S
Deposited: 8/27/2010
Determination: NMR
Clone: BupsA.00130.a.A1.GE25240
Protein: BupsA.00130.a.A1.PW25599
image of structure for 3UF8
3UF8
Deposited: 10/31/2011
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D242.GE00001
Protein: BupsA.00130.a.D242.PD00198
image of structure for 3UQB
3UQB
Deposited: 11/19/2011
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D214.GE00001
Protein: BupsA.00130.a.D214.PD00190
image of structure for 3UQA
3UQA
Deposited: 11/19/2011
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D220.GE00001
Protein: BupsA.00130.a.D220.PD00197
image of structure for 3VAW
3VAW
Deposited: 12/29/2011
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D24.GE00001
Protein: BupsA.00130.a.D24.PD00194
image of structure for 4DZ3
4DZ3
Deposited: 2/29/2012
Determination: XRay
Diffraction Image
Clone:
Protein: BupsA.00130.a.D227.PD00193
image of structure for 4DZ2
4DZ2
Deposited: 2/29/2012
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D239.GE00001
Protein: BupsA.00130.a.D239.PD00214
image of structure for 4FN2
4FN2
Deposited: 6/19/2012
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D214.GE00001
Protein: BupsA.00130.a.D214.PD00196
image of structure for 4G50
4G50
Deposited: 7/17/2012
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D214.GE00001
Protein: BupsA.00130.a.D214.PD00196
image of structure for 4GGQ
4GGQ
Deposited: 8/7/2012
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D21.GE00001
Protein: BupsA.00130.a.D21.PD00188
image of structure for 4GIV
4GIV
Deposited: 8/9/2012
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D214.GE00001
Protein: BupsA.00130.a.D214.PD00196
image of structure for 6O4A
6O4A
Deposited: 2/28/2019
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D21.GE00001
Protein: BupsA.00130.a.D21.PD00188
image of structure for 6O49
6O49
Deposited: 2/28/2019
Determination: XRay
Diffraction Image
Clone: BupsA.00130.a.D21.GE00001
Protein: BupsA.00130.a.D21.PD00188

Publications by SSGCID

The structure of a Burkholderia pseudomallei immunophilin-inhibitor complex reveals new approaches to antimicrobial development.
Harmer NJ, Norville IH, O'Shea K, Sarkar-Tyson M, Titball RW, Varani G, Zheng S
Biochem. J. - 2011
volume 437, issue 3, pages 413-22
PMID: 21574961; PMCID: PMC4045482
PDB: 2KE0, 2KO7
A structural biology approach enables the development of antimicrobials targeting bacterial immunophilins.
Abendroth J, Anderson V, Atkins K, Barnes SR, Begley DW, Edwards TE, Fox D, Harmer NJ, Holzgrabe U, Jenner D, Juli C, Lorimer DD, Moen SO, Muruthi M, Myler PJ, Norville IH, Pierce PG, Raymond AC, Safford K, Sarkar-Tyson M, Stacy R, Staker BL
Antimicrob. Agents Chemother. - 2013
volume 58, issue 3, pages 1458-67
PMID: 24366729; PMCID: PMC3957879

Citations by Others

Insights into immunophilin structure and function
C Lucke, M Weiwad - Current medicinal chemistry, 2011 - ingentaconnect.com
cited by: 12 others
PDB: 2KE0, 2KO7, 2L2S
In silico prediction of binding sites on proteins
S Leis, S Schneider, M Zacharias - Current medicinal chemistry, 2010 - ingentaconnect.com
cited by: 61 others
PDB: 2KE0
AIDA: ab initio domain assembly for automated multi-domain protein structure prediction and domain–domain interaction prediction
D Xu, L Jaroszewski, Z Li, A Godzik - Bioinformatics, 2015 - Oxford Univ Press
cited by: 1 other
PDB: 3UF8
Structure and Self-Assembly of the Calcium Binding Matrix Protein of Human Metapneumovirus
C Leyrat, M Renner, K Harlos, JT Huiskonen? - Structure, 2013 - Elsevier
cited by: 14 others
PDB: 3UF8
An Approximated Voxel Approach for the Identification and Modelling of Ligand-Binding Sites
LW Lee, A Bargiela - Journal of Physical Science and Application, 2012 - ico2s.org
PDB: 3UF8
To fuse or not to fuse: What is your purpose?
MR Bell, MJ Engleka, A Malik, JE Strickler - Protein Science, 2013 - Wiley Online Library
cited by: 25 others
PDB: 3UQB
Design of an expression system to enhance MBP-mediated crystallization
T Jin, W Chuenchor, J Jiang, J Cheng, Y Li - Scientific , 2017 - ncbi.nlm.nih.gov
cited by: 1 other
PDB: 4G50
Enzyme targets for drug design of new anti-virulence therapeutics
CM Kahler, M Sarkar-Tyson, EA Kibble- opinion in structural, 2018 - Elsevier
cited by: 2 others
PDB: 4GGQ
BECN2 interacts with ATG14 through a metastable coiledcoil to mediate autophagy
M Su, Y Li, S Wyborny, D Neau, S Chakravarthy - Protein , 2017 - Wiley Online Library
PDB: 4GGQ

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_126727
PATRIC ID: fig|320372.6.peg.4954
RefSeq: YP_336065
UniProt: Q3JK38

Enzyme & Pathway Information

Pathway Pathway ID EC Number
5.2.1.8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MTVVTTESGL KYEDLTEGSG AEARAGQTVS VHYTGWLTDG QKFDSSKDRN DPFAFVLGGG MVIKGWDEGV QGMKVGGVRR LTIPPQLGYG ARGAGGVIPP NATLVFEVEL LDV

NT Sequence
ATGACAGTCG TCACCACCGA ATCGGGCCTC AAATACGAAG ACCTGACCGA AGGCTCGGGC GCCGAGGCGC GCGCGGGCCA GACGGTGAGC GTGCACTACA CGGGCTGGCT CACCGACGGC CAGAAGTTCG ATTCGAGCAA GGATCGCAAT GATCCGTTCG CGTTCGTGCT CGGCGGCGGC ATGGTCATCA AGGGGTGGGA CGAAGGCGTG CAAGGGATGA AGGTGGGCGG CGTGCGCCGT CTGACGATCC CGCCGCAACT CGGCTACGGC GCGCGCGGCG CGGGCGGCGT GATTCCGCCC AATGCGACGC TCGTGTTCGA AGTCGAACTG CTCGACGTC

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