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Seattle Structural Genomics Center for Infectious Disease

ButhA.00168.a: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) (4-diphosphocytidyl-2C-methyl-D-erythritol synthase) (MEP cytidylyltransferase) (MCT) (BTH_I2089) (ispD)

Target Characteristics

from organism: Burkholderia thailandensis E264
most recent status: in PDB
center reference id: ButhA.00168.a
is community request: True
associated disease:
NIH risk group: 3
is select agent: False
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.00168.a.A1.GE32734 full length 1 236 GE32734
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

image of structure for 4YS8
4YS8
Deposited: 3/16/2015
Determination: XRay
Diffraction Image
Clone: ButhA.00168.a.A1.GE32734
Protein: ButhA.00168.a.A1.PW33338
image of structure for 4ZDQ
4ZDQ
Deposited: 4/18/2015
Determination: XRay
Diffraction Image
Clone: ButhA.00168.a.A1.GE32734
Protein: ButhA.00168.a.A1.PW33338

External Resources

Resource Reference ID
OrthoMCL: OG5_129829
PATRIC ID: fig|271848.6.peg.4901
RefSeq: YP_442613.1
UniProt: Q2SWT6

Enzyme & Pathway Information

Pathway Pathway ID EC Number
methylerythritol phosphate pathway I NONMEVIPP-PWY 2.7.7.60
superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) PWY-5121 2.7.7.60
isoprene biosynthesis I PWY-6270 2.7.7.60
superpathway of ergosterol biosynthesis II PWY-7156 2.7.7.60
taxadiene biosynthesis (engineered) PWY-7392 2.7.7.60
methylerythritol phosphate pathway II PWY-7560 2.7.7.60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MTSRLFALIP CAGTGSRSGS ALPKQYRTLA GRALLHYTLA AFDACSEFAQ TLVVISPDDA HFDARRFAGL RFAVRRCGGA SRQASVMNGL IQLAEFGATD ADWVLVHDAA RPGITPALIR TLIGALKDDP VGGIVALPVA DTLKRVPAGG DAIERTESRN GLWQAQTPQM FRIGMLRDAI QRAQLEGRDL TDEASAIEWA GHTPRVVQGS LRNFKVTYPE DFDLAEAILA HPARAS

NT Sequence
gtgacctccc gactcttcgc cctgattccg tgcgccggca ccggcagccg ctccggctcg gcattgccga agcaataccg cacgctcgcc ggccgcgcgc tcctgcatta cacgctcgcc gcgttcgacg cgtgcagcga gttcgcgcaa acgctcgtcg tgatttcccc cgacgacgcc cacttcgacg cccgccgctt tgccggtctg cgcttcgcgg tgcggcgctg cggcggcgcg tcccgacagg cgtcggtgat gaacgggctg atccagctcg ccgaattcgg cgcgacggac gccgactggg tgctcgtgca cgacgccgcg cgccccggca tcacgccggc gctgatccgc acgctgatcg gcgcgctgaa ggacgacccc gtcggcggca tcgtcgcgct gccggtcgcc gatacgctca agcgcgtgcc ggcgggcggc gacgcgatcg agcgcaccga atcgcgcaac ggcctctggc aggcgcagac gccgcagatg ttccgcatcg gcatgctgcg cgacgcgatc cagcgcgcgc agctcgaagg tcgcgatctg accgacgaag cgagcgcgat cgaatgggcg ggccatacgc cgcgcgtcgt gcagggcagt ctgcgcaact tcaaggtcac gtacccggag gatttcgacc tcgcggaggc gattctcgcg catccggcgc gcgccagttg a

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