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ButhA.17998.a: (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase ((3R)-hydroxymyristoyl ACP dehydrase) (EC 4.2.1.-) (BTH_I2038) (fabZ)

Target Characteristics

from organism: Burkholderia thailandensis E264
most recent status: in PDB
center reference id: ButhA.17998.a
is community request: True
associated disease:
NIH risk group: 3
is select agent: False
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.17998.a.A1.GE32877 full length 1 160 GE32877
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.17998.a.A1.PS01446 full length 1 160

Structures

image of structure for 4H4G
4H4G
Deposited: 9/17/2012
Determination: XRay
Diffraction Image
Clone: ButhA.17998.a.A1.GE32877
Protein: ButhA.17998.a.A1.PS01446

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

Citations by Others

Combination of type II fatty acid biosynthesis enzymes and thiolases supports a functional -oxidation reversal
JM Clomburg, SC Contreras, A Chou, JB Siegel- Metabolic, 2018 - Elsevier
cited by: 7 others
PDB: 4H4G
Structure of the Vibrio cholerae fatty acid regulator FadR
W Shi - 2015 - search.proquest.com
PDB: 4H4G

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_130367
PATRIC ID: fig|271848.6.peg.4848
RefSeq: YP_442562.1
UniProt: Q2SWY7

Enzyme & Pathway Information

Pathway Pathway ID EC Number
biotin biosynthesis I BIOTIN-BIOSYNTHESIS-PWY 4.2.1.59
(5Z)-dodecenoate biosynthesis I PWY0-862 4.2.1.59
superpathway of fatty acid biosynthesis I (E. coli) PWY0-881 4.2.1.59
cis-vaccenate biosynthesis PWY-5973 4.2.1.59
superpathway of mycolate biosynthesis PWY-6113 4.2.1.59
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) PWY-6282 4.2.1.59
superpathway of unsaturated fatty acids biosynthesis (E. coli) PWY-6284 4.2.1.59
superpathway of fatty acids biosynthesis (E. coli) PWY-6285 4.2.1.59
8-amino-7-oxononanoate biosynthesis I PWY-6519 4.2.1.59
gondoate biosynthesis (anaerobic) PWY-7663 4.2.1.59
oleate biosynthesis IV (anaerobic) PWY-7664 4.2.1.59
(5Z)-dodecenoate biosynthesis II PWY-7858 4.2.1.59
mycolate biosynthesis PWYG-321 4.2.1.59

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MRRTIMSTEK INFDIHKILT LLPHRYPILL VDRVLELEPH KSIKALKNVT VNEPFFTGHF PKRPVMPGVL IIEALAQAAA LLTFAEAEPK DPENTLYYFV GIDNARFKRV VEPGDQLILN VTFERYIRGI WKFKAVAEVD GKVAAEAELM CTVKTADAAP

NT Sequence
gtgagacgaa ccatcatgag cactgaaaaa atcaacttcg acattcacaa gatcctcacg ctgctgccgc accggtatcc gattctgctc gtcgatcgcg tgctcgaact cgaaccgcac aagtccatca aagcgttgaa gaacgtgacg gtcaacgagc cgttcttcac cggccacttc ccgaagcggc cggtgatgcc gggcgtgctg atcatcgaag cgctcgcgca ggcggccgcg ctcctcacgt tcgccgaggc ggagccgaag gatccggaga acacgctgta ttacttcgtc ggcatcgaca acgcgcgctt caagcgcgtc gtcgagccgg gcgatcaact gatcctgaac gtgacgttcg agcgctatat tcgcggcatc tggaagttca aggccgttgc ggaagtggac ggcaaggtcg cggcggaagc cgagctgatg tgcacggtca agacggccga cgcggcgccc tga

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