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Seattle Structural Genomics Center for Infectious Disease

CoimA.01230.a: Ornithine carbamoyltransferase, mitochondrial (EC=2.1.3.3) (Ornithine transcarbamylase) (OTCase)

Target Characteristics

from organism: Coccidioides immitis RS
most recent status: in PDB
center reference id: CoimA.01230.a
is community request: False
associated disease: California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
NIH risk group: 2
is select agent: True
NIH priority
pathogens category:
II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CoimA.01230.a.A1.GE28291 Full length( CoimA.01230.a ) 1 349 GE28291
CoimA.01230.a.MB1.GE30476 Full length( CoimA.01230.a ) 1 349 GE30476
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CoimA.01230.a.A1.PW28187 Full length( CoimA.01230.a ) 1 349
CoimA.01230.a.MB1.PW30353 Full length( CoimA.01230.a ) 1 349

Structures

image of structure for 3SDS
3SDS
Deposited: 6/9/2011
Determination: XRay
Diffraction Image
Clone: CoimA.01230.a.MB1.GE30476
Protein: CoimA.01230.a.MB1.PW30353

Publications by SSGCID

Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.
Choi R, Crowther GJ, Hewitt SN, Kelley A, Napuli AJ, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1006-9
PMID: 21904041; PMCID: PMC3169393
PDB: 3SDS, 3TL6, 3U40

Citations by Others


This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
EuPathDB: FungiDB:CIMG_04084
OrthoMCL: OG5_127715
RefSeq: XP_001244643.1
UniProt: P0CL21

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of arginine and polyamine biosynthesis ARG+POLYAMINE-SYN 2.1.3.3
L-arginine degradation V (arginine deiminase pathway) ARGDEGRAD-PWY 2.1.3.3
L-arginine degradation (Stickland reaction) ARGORNPROST-PWY 2.1.3.3
L-arginine biosynthesis II (acetyl cycle) ARGSYNBSUB-PWY 2.1.3.3
L-arginine biosynthesis I (via L-ornithine) ARGSYN-PWY 2.1.3.3
L-citrulline biosynthesis CITRULBIO-PWY 2.1.3.3
L-citrulline degradation CITRULLINE-DEG-PWY 2.1.3.3
L-proline biosynthesis II (from arginine) PWY-4981 2.1.3.3
urea cycle PWY-4984 2.1.3.3
superpathway of L-citrulline metabolism PWY-5004 2.1.3.3
L-arginine biosynthesis IV (archaebacteria) PWY-7400 2.1.3.3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MAKNQWRPYT NGSHAPSTPR HLLSIADLTP TEFATLVRNA SSYKKTIKSD SMPERLTGAL SGKTVAMMFS KRSTRTRVST EGAVVKMGGH PMFLGKDDIQ LGVNESLYDT SVVISSMVSC IVARVGPHSD IANLAKHSSV PVINALCDTF HPLQAIADFL TIHESFASQS ATHGTHPSSL GLEGLKIAWV GDANNVLFDL AIAATKMGVN VAVATPRGYE IPSHIVELIQ KAREGVQSPG NLTQTTVPEV AVKDADVIVT DTWISMGQET EKIKRLEAFK DFKVTSELAK RGGAKENWKF MHCLPRHPEE VSDEVFYSER SLVFPEAENR LWAAISALEA FVVNKGKIA

NT Sequence
ATGGCGAAAA ATCAGTGGAG ACCTTATACT AACGGCTCTC ATGCCCCTTC TACGCCTCGA CATCTTCTTT CCATTGCAGA CCTTACGCCC ACAGAATTCG CAACTCTCGT CCGAAATGCC TCTTCCTATA AGAAAACGAT CAAGTCGGAC TCAATGCCTG AGCGCTTGAC GGGGGCGCTG TCAGGAAAGA CCGTAGCCAT GATGTTCAGC AAACGCAGCA CCAGAACCAG AGTGTCAACC GAAGGCGCTG TGGTGAAGAT GGGAGGTCAT CCCATGTTTC TAGGAAAGGA TGATATCCAG TTGGGCGTGA ATGAGTCACT GTACGATACT TCTGTTGTAA TATCTTCAAT GGTATCTTGC ATTGTTGCCC GCGTGGGACC TCACTCCGAC ATTGCCAATT TGGCTAAACA CTCGAGTGTG CCGGTTATTA ACGCCTTATG TGACACCTTC CATCCGCTGC AAGCAATCGC CGATTTCCTC ACCATCCATG AATCCTTTGC GTCACAATCT GCAACACACG GAACACATCC CTCGAGCCTG GGATTGGAGG GACTGAAAAT TGCGTGGGTC GGAGATGCAA ATAATGTCCT TTTTGACCTG GCGATCGCCG CGACCAAGAT GGGTGTAAAT GTGGCTGTTG CGACACCCAG GGGCTACGAG ATTCCGTCTC ATATCGTTGA ACTGATTCAG AAAGCACGAG AAGGGGTCCA GTCACCAGGA AATCTCACAC AAACCACCGT GCCAGAAGTC GCCGTCAAGG ATGCAGACGT CATCGTGACC GACACCTGGA TCTCGATGGG GCAAGAGACC GAGAAAATCA AAAGACTGGA AGCGTTCAAA GACTTTAAAG TGACTTCAGA GCTTGCTAAA CGAGGAGGCG CAAAAGAGAA TTGGAAGTTC ATGCATTGCT TACCACGACA TCCCGAGGAG GTTAGCGACG AAGTTTTCTA TAGCGAGAGA TCTCTCGTCT TCCCAGAGGC CGAAAATCGA CTATGGGCGG CCATTTCGGC GCTGGAAGCG TTTGTTGTTA ACAAGGGGAA GATTGCT

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