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Seattle Structural Genomics Center for Infectious Disease

CrneC.00629.a: Acetyl-coenzyme A synthetase (EC 6.2.1.1)

Target Characteristics

from organism: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
most recent status: in PDB
center reference id: CrneC.00629.a
is community request: True
associated disease: Pneumonia-like illness
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrneC.00629.a.FS11.GE00458 full length 3 681 GE00458
CrneC.00629.a.HA11.GE00478 full length 46 679 GE00478
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrneC.00629.a.FS11.PD00402 full length 3 681

Structures

image of structure for 5IFI
5IFI
Deposited: 2/26/2016
Determination: XRay
Diffraction Image
Clone: CrneC.00629.a.FS11.GE00458
Protein: CrneC.00629.a.FS11.PD00402
image of structure for 5K85
5K85
Deposited: 5/27/2016
Determination: XRay
Diffraction Image
Clone: CrneC.00629.a.FS11.GE00458
Protein: CrneC.00629.a.FS11.PD00402
image of structure for 5K8F
5K8F
Deposited: 5/30/2016
Determination: XRay
Diffraction Image
Clone: CrneC.00629.a.FS11.GE00458
Protein: CrneC.00629.a.FS11.PD00402
image of structure for 5U29
5U29
Deposited: 11/30/2016
Determination: XRay
Diffraction Image
Clone: CrneC.00629.a.FS11.GE00458
Protein: CrneC.00629.a.FS11.PD00402
image of structure for 5VPV
5VPV
Deposited: 5/5/2017
Determination: XRay
Diffraction Image
Clone: CrneC.00629.a.FS11.GE00458
Protein: CrneC.00629.a.FS11.PD00402

External Resources

Resource Reference ID
EuPathDB: FungiDB:CNAG_00797
RefSeq: XP_012046967.1
UniProt: J9VFT1

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of acetate utilization and formation ACETATEUTIL-PWY 6.2.1.1
acetate conversion to acetyl-CoA PWY0-1313 6.2.1.1
L-isoleucine biosynthesis V PWY-5108 6.2.1.1
lupulone and humulone biosynthesis PWY-5132 6.2.1.1
colupulone and cohumulone biosynthesis PWY-5133 6.2.1.1
superpathway of bitter acids biosynthesis PWY-5134 6.2.1.1
ethanol degradation III PWY66-161 6.2.1.1
ethanol degradation IV PWY66-162 6.2.1.1
ethanol degradation II PWY66-21 6.2.1.1
cis-genanyl-CoA degradation PWY-6672 6.2.1.1
chitin degradation to ethanol PWY-7118 6.2.1.1
adlupulone and adhumulone biosynthesis PWY-7857 6.2.1.1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MGKTEVAPGV HHVHPLPDSV PESEDLFAPP PRMQGKEGRP KPHIGPNYES YVKEWAKTVG PNSDEWWAAK ARETLDWYDD FKTVRAGGFE HGDVQWFPEG TLNAAYNCLD RHYYKNPKKT AIIYEADEPS ESREVSYEEL MQETCRVANV LKSYGVKKGD AVSIYLPMTW QAAAAFLACA RIGAIHSAVF AGFSAESLRD RVNDCECKVL ITTDEGRRGG KTIATKQIVD AALQQCPLVE NVLVLRRTGN KVPMTEGRDK WWDEECAKMP AYCPCERMAS EDPLFILYTS GSTGKPKGVV HSTAGYLLGT ALTLKYVFDA HPDDRFACMA DIGWITGHSY IIYGPLANGI TTAVFESTPV YPTPSRYWDF VDKWKATQLY TAPTAIRLLR RMGEDHVKNH DLSSLRVLGS VGEPINPEAW HWYNDFAGKN QCAIVDTYWM TETGSISIAP LPGAISTKPG SATFPFFGMD VDIIDPQTGQ VLEGNDVEGV LVARRPWPSI ARTVYRDHKR YLETYMKPYP GYFFFGDGAA RDYDGYMWIK GRVDDVINVS GHRLSTAEVE SALILHKGVA ETAVVGCADD LTGQAVYAFV TMKPEFDLKA TKEADLSKEL AIQVRKVIGP FAAPKKIYLV SDLPKTRSGK IMRRVLRKIV AGEGDQLGDL SSIADPQIVE EVKQKVTGSA

NT Sequence
atgggcaaga cagaagtcgc acccggcgtc caccacgtcc accctctccc cgactccgtc cccgagtctg aggacctctt cgctccccct ccccgaatgc aggggaagga aggccgcccg aagcctcaca tcggtcctaa ctacgagtcc tacgtcaagg aatgggccaa gactgttggt cccaatagtg atgagtggtg ggccgcaaag gccagggaaa ctcttgattg gtacgacgac tttaagactg tcagggccgg tggctttgag catggcgacg ttcagtggtt ccccgaaggt accttgaacg ccgcctacaa ctgtcttgac cgacactact acaagaaccc caaaaagacc gccatcatct acgaggctga cgagccttcc gagtcccgtg aggtctctta cgaggagctc atgcaggaga cttgccgagt tgccaacgtc ctcaagagct acggtgtgaa gaagggtgac gctgtgtcca tctatcttcc catgacttgg caagccgctg ctgcgttcct tgcttgtgcc agaatcggtg ccatccactc tgctgtcttt gccggtttta gtgccgagag tttgagggat agggtcaatg attgtgaatg caaggttttg atcaccactg acgagggtcg acgaggtggt aagactattg ctaccaagca gatcgtcgat gccgctctcc agcaatgccc ccttgttgag aacgttctcg ttcttcgacg aaccggcaac aaggtcccca tgactgaggg ccgtgacaaa tggtgggacg aagagtgtgc caagatgccc gcttactgtc cttgcgagag gatggcttcc gaggatcctc tcttcatcct ctacacctct ggttccaccg gtaagccgaa gggtgttgtc cactccactg ctggttacct cctcggcacc gcccttaccc tcaagtacgt ctttgacgct catcctgatg accgattcgc ctgtatggcc gatatcggat ggattacggg tcacagttac atcatctacg gccctctcgc caacggcatc accaccgccg tctttgaatc cacccccgtc tatcccacac catccagata ctgggacttt gttgacaagt ggaaagccac tcagctctac actgcgccca ctgcgatccg attgttaaga cgtatgggcg aggaccacgt gaagaaccac gatctcagct ctctccgagt tttgggctct gttggtgagc ccatcaaccc cgaggcttgg cattggtaca atgactttgc gggcaagaat caatgtgcca ttgttgacac atactggatg accgagactg gttctatttc tattgctcct ctccctggag ccatctccac caagcccggt tctgccactt tccccttctt cggtatggat gttgacatca ttgaccctca aactggtcag gtcttggagg gtaacgatgt agagggtgtt ctcgttgcca ggaggccatg gcccagtatt gccaggactg tctacaggga ccacaagagg tatcttgaga cttacatgaa gccttacccc ggctacttct tctttggtga tggtgctgcc cgagactatg atggttacat gtggatcaag ggacgagttg atgatgttat caacgtgtct ggtcaccgac tttccacggc cgaggtcgag tctgccctta tccttcacaa gggtgtcgcc gagactgccg ttgttggatg cgctgatgac cttactggtc aggctgttta cgctttcgtt accatgaagc ccgagtttga tctaaaggct accaaggagg ccgaccttag caaggagttg gctatccagg ttaggaaggt tattggtccc tttgccgctc ccaagaagat ttaccttgtt tccgatctcc ccaagacccg atcaggaaag atcatgcgtc gagttctccg aaagattgtc gctggcgagg gtgaccaatt gggagatctt tcttccatcg ctgaccccca aattgtcgaa gaagtcaagc aaaaggttac cggctctgct taa

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