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Seattle Structural Genomics Center for Infectious Disease

EnhiA.01296.a: Histidine triad nucleotide-binding protein

Target Characteristics

from organism: Entamoeba histolytica HM-1:IMSS
most recent status: in PDB
center reference id: EnhiA.01296.a
is community request: False
associated disease: Amoebic dysentery
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EnhiA.01296.a.A1.GE28428 Full length( EnhiA.01296.a ) 1 113 GE28428
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EnhiA.01296.a.A1.PS00632a Full length( EnhiA.01296.a ) 1 113

Structures

image of structure for 3OMF
3OMF
Deposited: 8/26/2010
Determination: XRay
Diffraction Image
Clone:
Protein: EnhiA.01296.a.A1.PS00632
image of structure for 3OJ7
3OJ7
Deposited: 8/20/2010
Determination: XRay
Diffraction Image
Clone:
Protein: EnhiA.01296.a.A1.PS00632
image of structure for 3OXK
3OXK
Deposited: 9/21/2010
Determination: XRay
Diffraction Image
Clone:
Protein: EnhiA.01296.a.A1.PS00632

Publications by SSGCID

Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP.
Abramov A, Choi R, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Nakazawa Hewitt S, Staker BL, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 572-6
PMID: 25945711; PMCID: PMC4427167
PDB: 3OJ7, 3OMF, 3OXK

Citations by Others

Upgrading and Validation of the AMBER Force Field for Histidine and Cysteine Zinc (II)-Binding Residues in Sites with Four Protein Ligands
M Macchiagodena, M Pagliai, C Andreini- Journal of chemical, 2019 - ACS Publications
cited by: 12 others
PDB: 3OJ7
Crystal Structure of Histidine Triad Nucleotide-Binding Protein from the Pathogenic Fungus Candida albicans
A Jung, JS Yun, S Kim, SR Kim, M Shin- Molecules and, 2019 - ncbi.nlm.nih.gov
PDB: 3OJ7
Rare Sidechain Conformations in Proteins and DNA
BJ Hintze - 2015 - search.proquest.com
PDB: 3OJ7
Expression, purification, crystallization and preliminary X-ray crystallographic analysis of human histidine triad nucleotide-binding protein 2 (hHINT2)
R Dolot, A Wlodarczyk, GD Bujacz? - Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2013 - scripts.iucr.org
cited by: 1 other
PDB: 3OJ7
Principal component analysis of alpha-helix deformations in transmembrane proteins
A Bevacqua, S Bakshi, Y Xia- PloS one, 2021 - journals.plos.org
PDB: 3OXK
Structural Biology for Drug Design: Applications in Two Systems
KM Maize - 2016 - search.proquest.com
PDB: 3OXK
Response to Errors in Crystal structure of HINT from Helicobacter pylori
KF Tarique, S Devi, SA Abdul Rehman - Section F: Structural , 2016 - scripts.iucr.org
PDB: 3OXK
Structural characterization of human histidine triad nucleotide-binding protein 2, a member of the histidine triad superfamily
KM Maize, CR Wagner, BC Finzel - FEBS Journal, 2013 - Wiley Online Library
cited by: 4 others
PDB: 3OXK

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
EuPathDB: AmoebaDB:EHI_093910
OrthoMCL: OG5_126794
RefSeq: XP_655618.1
UniProt: C4LYI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MADSCIFCKI AQKQIPSTIV YEDDEIFAFK DINPIAPIHI LVIPKQHIAS LNEITEENEA FIGKVLYKVS LIGKKECPEG YRVVNNIGED AGQTVKHIHF HILGGKKLAW DKL

NT Sequence
ATGGCTGATT CATGCATTTT CTGTAAGATT GCACAAAAAC AAATTCCATC AACTATTGTT TATGAAGATG ATGAAATTTT TGCTTTTAAA GATATTAACC CAATTGCTCC TATTCATATT CTTGTTATTC CTAAACAACA CATTGCTTCT TTAAATGAAA TAACTGAAGA AAATGAGGCT TTTATTGGTA AAGTTTTGTA CAAAGTCTCT TTAATTGGAA AGAAAGAATG TCCTGAAGGA TATCGTGTGG TTAATAATAT TGGTGAAGAT GCTGGTCAAA CAGTTAAACA TATTCATTTC CATATTCTTG GAGGAAAAAA ACTTGCTTGG GATAAATTA

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