MymaA.17645.a: RNA polymerase sigma factor
Target Characteristics
from organism:
Mycobacterium marinum ATCC BAA-535 / M
most recent status:
purified
center reference id:
MymaA.17645.a
is community request:
True
associated disease:
Cutaneous ulcerations, deep abscesses
NIH risk group:
2
is select agent:
False
NIH priority
IIIC
Ordering Clones & Proteins
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Clones*
CENTER
DOMAIN/REGION
INFO
AA
AA
ORDER
MymaA.17645.a.B1.GE39182
full length
more 1
255
GE39182
MymaA.17645.a.MB1.GE39285
full length
more 1
255
GE39285
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER
DOMAIN/REGION
INFO
AA
AA
ORDER
MymaA.17645.a.MB1.PW37771
full length
more 1
255
PW37771
External Resources Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSTSTSLLGE ERLGRFPASP ASVATLSGGT STEGTVFIKM ADIDGVTLAE APDPEPPEET DEQLTARFER DAIPLLDQLY GGALRMTRNP ADAEDLLQET MVKAYAGFRS FREGTNLKAW LYRILTNTYI NSYRKKQRQP AEYPTDEITD WQLASNAEHS STGLRSAEVE ALEALPDTEI KEALQALPEE FRMAVYYADV EGFPYKEIAE IMDTPIGTVM SRLHRGRRQL RTLLADVAKE RGFVRGEQAH QEVSS
NT Sequence
gtgtcgacct cgacgagcct gctgggcgag gagcggttgg ggcgcttccc tgcgagtccg gcgtcggtag cgacgctgtc cggtggcacc tcgaccgaag ggaccgtgtt tatcaagatg gccgacatcg atggtgtgac cctggctgag gcgcccgacc cggagccgcc tgaggagact gacgagcagc tgactgcgcg cttcgagcgc gacgcgattc ccctgttgga ccagctgtac ggcggcgcgc tgcggatgac gcgcaatccg gccgatgccg aggatctgct gcaggagacc atggtcaagg cctacgcggg ttttcgttct ttccgggagg gcaccaatct caaagcgtgg ctgtaccgga ttctgaccaa tacctacatc aacagctatc gcaagaagca gcgacagccc gcggaatatc ccaccgacga gatcaccgat tggcagctgg catccaacgc cgagcattcc tcgaccgggc tgcgttcggc cgaggtcgag gccctcgagg cgttgccgga cactgagata aaagaggcgc tgcaggcatt gccggaagaa ttccggatgg cggtctacta cgccgacgtg gagggttttc cctacaagga gatcgccgag atcatggaca cgccgatcgg gactgtgatg tcgcggctac accggggcag acggcagctg cgcaccctgt tggcggatgt ggccaaggaa cgtggcttcg tccgcggtga gcaggcccac caggaggtgt cttcatga
purified on: 8/17/2015
concentration: 23.5mg/ml
observed molecular weight:
80kDa
expression level:
High Expression
protein purification buffer:
500mM NaCl, 25mM HEPES, 5% Glycerol, 0.25 % azide, 2mM DTT pH 7.00
expression host:
data unavailable
vial count (approx.):
2
vial volume:
110µl
percent identity: 99
percent coverage: 38
MVSTSTSLLG EERLGRFPAS PASVATLSGG TSTEGTVFIK MADIDGVTLA EAPDPEPPEE TDEQLTARFE RDAIPLLDQL YGGALRMTRN PADAEDLLQE TMVKAYAGFR SFREGTNLKA WLYRILTNTY INSYRKKQRQ PAEYPTDEIT DWQLASNAEH SSTGLRSAEV EALEALPDTE IKEALQALPE EFRMAVYYAD VEGFPYKEIA EIMDTPIGTV MSRLHRGRRQ LRTLLADVAK ERGFVRGEQA HQEVSS
CAGCNNNNNT CAGACTGNNN ATGNNNNNNN NNNNNNCNCG CAGNNNNNNN NNAGCTNNNN NNNNAANNNN NNCAACNNNN NCAACNNNNN NNGGATCGAG GGAAGGATTT CAGAAGGTAN NNNNNAAGCT CAGACCCAGG GTCCTGGTTC GATGGTGTCG ACCTCGACGA GCCTGCTGGG CGAGGAGCGG TTGGGGCGCT TCCCTGCGAG TCCGGCGTCG GTAGCGACGC TGTCCGGTGG CACCTCGACC GAAGGGACCG TGTTTATCAA GATGGCCGAC ATCGATGGTG TGACCCTGGC TGAGGCGCCC GACCCGGAGC CGCCTGAGGA GACTGACGAG CAGCTGACTG CGCGCTTCGA GCGCGACGCG ATTCCCCTGT TGGACCAGCT GTACGGCGGC GCGCTGCGGA TGACGCGCAA TCCGGCCGAT GCCGAGGATC TGCTGCAGGA GACCATGGTC AAGGCCTACG CGGGTTTTCG TTCTTTCCGG GAGGGCACCA ATCTCAAAGC GTGGCTGTAC CGGATTCTGA CCAATACCTA CATCAACAGC TATCGCAAGA AGCAGCGACA GCCCGCGGAA TATCCCACCG ACGAGATCAC CGATTGGCAG CTGGCATCCA ACGCCGAGCA TTCCTCGACC GGGCTGCGTT CGGCCGAGGT CGAGGCCCTC GAGGCGTTGC CGGACACTGA GATAAAAGAG GCGCTGCAGG CATTGCCGGA AGAATTCCGG ATGGCGGTCT ACTACGCCGA CGTGGAGGGT TTTCCCTACA AGGAGATCGC CGAGATCATG GACACGCCGA TCGGGACTGT GATGTCGCGG CTACACCGGG GCAGACGGCA GCTGCGCACC CTGTTGGCGG ATGTGGCCAA GGAACGTGGC TTCGTCCGCG GTGAGCAGGC CCACCAGGAG GTGTCTTCAT AAT
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MSTSTSLLGE ERLGRFPASP ASVATLSGGT STEGTVFIKM ADIDGVTLAE APDPEPPEET DEQLTARFER DAIPLLDQLY GGALRMTRNP ADAEDLLQET MVKAYAGFRS FREGTNLKAW LYRILTNTYI NSYRKKQRQP AEYPTDEITD WQLASNAEHS STGLRSAEVE ALEALPDTEI KEALQALPEE FRMAVYYADV EGFPYKEIAE IMDTPIGTVM SRLHRGRRQL RTLLADVAKE RGFVRGEQAH QEVSS
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAATAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGTGTC GACCTCGACG AGCCTGCTGG GCGAGGAGCG GTTGGGGCGC TTCCCTGCGA GTCCGGCGTC GGTAGCGACG CTGTCCGGTG GCACCTCGAC CGAAGGGACC GTGTTTATCA AGATGGCCGA CATCGATGGT GTGACCCTGG CTGAGGCGCC CGACCCGGAG CCGCCTGAGG AGACTGACGA GCAGCTGACT GCGCGCTTCG AGCGCGACGC GATTCCCCTG TTGGACCAGC TGTACGGCGG CGCGCTGCGG ATGACGCGCA ATCCGGCCGA TGCCGAGGAT CTGCTGCAGG AGACCATGGT CAAGGCCTAC GCGGGTTTTC GTTCTTTCCG GGAGGGCACC AATCTCAAAG CGTGGCTGTA CCGGATTCTG ACCAATACCT ACATCAACAG CTATCGCAAG AAGCAGCGAC AGCCCGCGGA ATATCCCACC GACGAGATCA CCGATTGGCA GCTGGCATCC AACGCCGAGC ATTCCTCGAC CGGGCTGCGT TCGGCCGAGG TCGAGGCCCT CGAGGCGTTG CCGGACACTG AGATAAAAGA GGCGCTGCAG GCATTGCCGG AAGAATTCCG GATGGCGGTC TACTACGCCG ACGTGGAGGG TTTTCCCTAC AAGGAGATCG CCGAGATCAT GGACACGCCG ATCGGGACTG TGATGTCGCG GCTACACCGG GGCAGACGGC AGCTGCGCAC CCTGTTGGCG GATGTGGCCA AGGAACGTGG CTTCGTCCGC GGTGAGCAGG CCCACCAGGA GGTGTCTTCA TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
