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Seattle Structural Genomics Center for Infectious Disease

MysmA.00213.a: Delta3,5-delta2,4-dienoyl-CoA isomerase (enoyl-CoA hydratase) (Ech)

Target Characteristics

from organism: Mycobacterium smegmatis ATCC 700084 / mc(2)155
most recent status: native diffraction-data
center reference id: MysmA.00213.a
is community request: False
associated disease:
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.00213.a.A1.GE28584 Full length( MysmA.00213.a ) 1 271 GE28584
MysmA.00213.a.B1.GE43075 Full length( MysmA.00213.a ) 1 271 GE43075
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.00213.a.A1.PS00547 Full length( MysmA.00213.a ) 1 271

External Resources

Resource Reference ID
OrthoMCL: OG5_129459
PATRIC ID: fig|246196.19.peg.6184
RefSeq: YP_890568.1
UniProt: A0R5Y0

Enzyme & Pathway Information

Pathway Pathway ID EC Number
fatty acid beta-oxidation I FAO-PWY 4.2.1.17
L-isoleucine degradation I ILEUDEG-PWY 4.2.1.17
oleate beta-oxidation PWY0-1337 4.2.1.17
phenylacetate degradation I (aerobic) PWY0-321 4.2.1.17
benzoyl-CoA degradation I (aerobic) PWY-1361 4.2.1.17
fermentation to 2-methylbutanoate PWY-5109 4.2.1.17
fatty acid beta-oxidation II (peroxisome) PWY-5136 4.2.1.17
unsaturated, even numbered fatty acid beta-oxidation PWY-5138 4.2.1.17
superpathway of glyoxylate cycle and fatty acid degradation PWY-561 4.2.1.17
superpathway of phenylethylamine degradation PWY-6071 4.2.1.17
4-hydroxybenzoate biosynthesis III (plants) PWY-6435 4.2.1.17
fatty acid beta-oxidation VI (peroxisome) PWY66-391 4.2.1.17
pyruvate fermentation to hexanol (engineered) PWY-6863 4.2.1.17
superpathway of cholesterol degradation I (cholesterol oxidase) PWY-6928 4.2.1.17
superpathway of testosterone and androsterone degradation PWY-6937 4.2.1.17
androstenedione degradation PWY-6944 4.2.1.17
superpathway of cholesterol degradation II (cholesterol dehydrogenase) PWY-6947 4.2.1.17
methyl ketone biosynthesis (engineered) PWY-7007 4.2.1.17
4-coumarate degradation (anaerobic) PWY-7046 4.2.1.17
fatty acid salvage PWY-7094 4.2.1.17
jasmonic acid biosynthesis PWY-735 4.2.1.17
docosahexaenoate biosynthesis III (6-desaturase, mammals) PWY-7606 4.2.1.17
(8E,10E)-dodeca-8,10-dienol biosynthesis PWY-7654 4.2.1.17
Spodoptera littoralis pheromone biosynthesis PWY-7656 4.2.1.17
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) PWY-7726 4.2.1.17
toluene degradation to benzoyl-CoA (anaerobic) PWY-81 4.2.1.17
L-valine degradation I VALDEG-PWY 4.2.1.17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MADTYESVTV EVKDHVAQVT LIGPGKGNAM GPAFWEEMPD VFGTLDDDPD VRAIVLTGSG KNFSYGLDLP AMGASLPALD AGAKSRSDFH KRLQKMQGAI TAVADCRTPT IASVHGWCIG GGVDLISAVD IRYASADAKF SVREVKLAIV ADVGSLARLP LILSDGHLRE LALTGKDIDA ARAEKIGLVN DVYDDAEASL AAAHATAAEI AANPPLTVAG VKDVLDAQRT AQVAESLRYV AAWNSAFLPS KDLAEAVTAM FEKRRPEFTG E

NT Sequence
ATGGCTGACA CGTACGAGTC GGTGACCGTC GAGGTCAAAG ACCATGTCGC ACAGGTGACC CTGATCGGGC CCGGCAAGGG CAACGCGATG GGGCCCGCGT TCTGGGAAGA GATGCCCGAC GTGTTCGGCA CCCTCGACGA CGATCCCGAC GTACGCGCCA TCGTGCTGAC CGGCTCCGGC AAGAACTTCA GCTACGGCCT GGACCTGCCC GCCATGGGCG CCTCGCTGCC CGCCCTGGAC GCGGGCGCGA AGTCACGCTC CGACTTCCAC AAGCGCCTGC AGAAGATGCA GGGCGCCATC ACCGCGGTCG CCGACTGCCG CACGCCCACC ATCGCATCGG TGCACGGCTG GTGCATCGGC GGCGGCGTGG ACCTCATCAG CGCCGTGGAC ATCCGTTACG CGAGCGCCGA CGCCAAGTTC TCGGTGCGTG AGGTCAAGCT CGCGATCGTC GCCGACGTGG GCAGCCTCGC GCGTCTGCCG CTGATCCTCA GCGACGGACA TCTGCGCGAA TTGGCGCTCA CCGGCAAGGA CATCGACGCC GCACGGGCCG AGAAGATCGG TCTGGTCAAC GACGTCTACG ACGACGCCGA GGCCTCGCTG GCCGCCGCGC ACGCCACGGC CGCCGAGATC GCCGCCAACC CGCCGCTGAC GGTCGCAGGC GTCAAGGACG TGCTCGACGC GCAGCGCACC GCGCAGGTGG CCGAGAGCCT GCGGTACGTC GCGGCCTGGA ACTCGGCGTT CCTGCCGTCG AAGGACCTCG CCGAGGCGGT GACGGCGATG TTCGAAAAGC GCAGGCCCGA GTTCACCGGC GAG

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