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Seattle Structural Genomics Center for Infectious Disease

MysmA.00725.a: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE)

Target Characteristics

from organism: Mycobacterium smegmatis ATCC 700084 / mc(2)155
most recent status: purified
center reference id: MysmA.00725.a
is community request: True
associated disease:
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.00725.a.B1.GE40129 Full length( MysmA.00725.a ) 1 322 GE40129
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.00725.a.B1.PS38006 Full length( MysmA.00725.a ) 1 322

External Resources

Resource Reference ID
OrthoMCL: OG5_130885
PATRIC ID: fig|246196.19.peg.5297
RefSeq: YP_889675.1
UniProt: A0R3D7

Enzyme & Pathway Information

Pathway Pathway ID EC Number
methylerythritol phosphate pathway I NONMEVIPP-PWY 2.7.1.148
superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) PWY-5121 2.7.1.148
isoprene biosynthesis I PWY-6270 2.7.1.148
superpathway of ergosterol biosynthesis II PWY-7156 2.7.1.148
taxadiene biosynthesis (engineered) PWY-7392 2.7.1.148
methylerythritol phosphate pathway II PWY-7560 2.7.1.148

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MAVPASDGST ASEWVTTGSV TVRVPGKVNL YLSVGDLRDD GYHDLTTVFH AVSLLDEVTV RNADVLSLRM IGEGAESLPA DERNLAWRAA ELMAEHVGRA PDVEITIEKS IPVAGGMAGG SADAAGVLVA MNTLWELGVP RRDLHALAAQ LGSDVPFALH GGTALGSGRG EDLATVLARN TFHWVLAFAH RGLSTPKVFG ELDRLRADTT RGLPRTDEPE PVLAALASGD PAELAALLGN DLQPAALSLY PDLRRTLRAG VEAGALAGIV SGSGPTCAFL CASAPAALDV GTQLSGAGVC RTVRVASGPV HGAHVVPAPS AS

NT Sequence
GTGGCCGTGC CCGCATCCGA TGGCAGTACC GCTTCCGAAT GGGTGACCAC CGGCTCGGTC ACCGTGCGCG TCCCCGGCAA GGTGAACCTG TACCTGTCCG TGGGCGATCT GCGCGACGAC GGGTACCACG ATCTGACGAC GGTGTTCCAC GCGGTCTCGC TGCTCGACGA GGTGACGGTC CGCAACGCCG ACGTGCTGTC GCTGCGGATG ATCGGGGAGG GCGCCGAGTC GCTGCCGGCC GACGAGCGCA ATCTGGCGTG GCGCGCCGCC GAGTTGATGG CCGAGCACGT CGGCCGCGCC CCCGATGTCG AGATCACGAT CGAGAAGTCC ATCCCGGTGG CCGGCGGGAT GGCCGGTGGC TCGGCGGATG CCGCGGGCGT GCTGGTCGCG ATGAACACGC TGTGGGAGCT CGGCGTGCCG CGGCGGGATC TGCACGCGCT GGCCGCACAG CTGGGCAGTG ATGTGCCGTT CGCGCTGCAC GGCGGGACCG CGCTGGGGTC CGGGCGCGGC GAGGACCTCG CCACGGTACT CGCGCGCAAC ACGTTCCACT GGGTGCTGGC GTTCGCGCAC CGTGGCTTGT CGACGCCCAA GGTCTTCGGG GAACTGGACC GGTTGCGCGC CGACACCACC CGTGGTCTGC CGCGCACCGA CGAGCCCGAG CCCGTGCTGG CCGCGCTGGC CTCGGGCGAT CCGGCCGAGC TGGCGGCCCT GCTGGGCAAC GATCTGCAGC CCGCGGCGCT GAGCCTGTAT CCCGACCTGC GTCGCACGCT GCGTGCCGGT GTCGAGGCGG GTGCGCTCGC GGGCATCGTG TCGGGGTCCG GTCCGACGTG TGCGTTCCTG TGTGCGTCGG CCCCGGCCGC ACTGGATGTC GGCACGCAGC TGTCGGGGGC CGGGGTGTGC CGCACCGTGC GCGTGGCGAG CGGGCCTGTA CACGGCGCGC ACGTCGTTCC CGCGCCGTCG GCATCG

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