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Seattle Structural Genomics Center for Infectious Disease

MysmA.17261.a: recB Exodeoxyribonuclease V, beta subunit (EC 3.1.11.5)

Target Characteristics

from organism: Mycobacterium smegmatis ATCC 700084 / mc(2)155
most recent status: diffraction
center reference id: MysmA.17261.a
is community request: True
associated disease:
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.17261.a.A1.GE30128 full length 1 1083 GE30128
MysmA.17261.a.A2.GE30129 UvrD-like helicase 1 323 GE30129
MysmA.17261.a.A3.GE30130 UvrD-like helicase C-terminal domain 349 607 GE30130
MysmA.17261.a.A4.GE30131 Phage-type/RecB exonuclease C-terminal 754 1081 GE30131
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.17261.a.A2.PS00802 UvrD-like helicase 1 323
MysmA.17261.a.A4.PS00845 Phage-type/RecB exonuclease C-terminal 754 1081

External Resources

Resource Reference ID
OrthoMCL: OG5_140510
PATRIC ID: fig|246196.19.peg.1314
RefSeq: YP_885717.1
UniProt: A0QS29

Enzyme & Pathway Information

Pathway Pathway ID EC Number
3.1.11.5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MKVFDLLGPL PAPNTTTVLE ASAGTGKTFA LAGLVTRFVA EGVATLDQML LITFGRAASQ ELRERVRAQI VAALVALDDP SRACNDLEEY LVKTDQQARR RRLRDALAGF DAATIATTHQ FCQIVLKSLG VAGDSDAGVT LVESLDDLVS EIVDDLYLAH FGGQKDDPEL SYPEALKLAR VVVGNPATQL RPRDPDPDSP AAVRLKFARD VLAELEIRKR RRGVLGYDDL LTRLADALEP EDSPARVRMQ QRWPIVMVDE FQDTDPVQWQ VIERAFSGRS TLVLIGDPKQ AIYAFRGGDI ATYLRAAATA GDKQTLGTNW RSDRALVDRL QAVLRGAQLG GPDIVVHDVQ ARHQGHRLVG APRNDPFRLR VVSRKPGNTR VIPIDQLRRH IGRDLAADIS ALLNSGATWC DQPVQAKDIA VITETHKDAR ACHAALLAAG IPAVYTGDSD VFTSEAAEDW LYLLEAFDQP HRPGLVRAAA ATMFFGETAE SLAAGGDALT DRVADTLREW AGHARERGVA AIFEAAQLAG MGKRVLSWQG GERLMTDLAH MTQLLQDTAH REGFGLAALR DWLRTQRSER GGESERNRRL DSDAAAVQIM TVWVSKGLQF PVVYLPFAFN RYVPEPDLVL FHDDGQRCLH VGGADPAVAR AGRAEAAGDD SRLTYVALTR AQSQVVAWWA PSYDEPNGGL SRLMRGRAPG EAIVPDKCSP PKISDEDALE RLRAWEAAGG PVIEESVIGA VSPVPPEPAP EDLAARKFFR AIDMAWKRTS YSGLLRAAET AGVGVSSEPE VTERDDEFDD IPVVAPAEGA DVPSPMAHLP TGAAFGSLVH AVLETADPFA EDLTAELATH IDAHSQHWPV EVETAELAAA LVPMHDTPLG PLAPGLTLRQ IGLRDRLCEL DFEFPMAGGD LRGGRFARLS DVGELLREYL PADDPLAVYA ERLSTGILGV QPLRGYLSGS VDAVLRVGEK FVIVDYKTNW LGTGDGTLTA ADYGRRRMVE AMLHSDYPLQ ALLYAVVLHR YLGWRLSGYD PATHLGGVLY LFVRGMCGAG TPVVDGHPAG VFSWEPPADL VVALSKLLDA EAP

NT Sequence
gtgaaggttt tcgatctgct cggcccgctg cccgcaccga acaccaccac ggtgctggag gccagcgcgg gcaccggcaa gacgttcgcg ctggccggtc tggtcactcg ttttgtcgcc gagggcgtcg cgacgctcga ccagatgctg ctcatcacgt tcggccgcgc cgccagccag gaactgcgcg agcgtgtgcg cgcccagatc gtcgccgcgc tcgtcgcgtt ggacgacccg tcgcgggcct gcaacgacct ggaggaatac ctggtcaaga ccgaccagca ggcgcggcgc cggcgcctgc gcgacgcgct ggccgggttc gacgccgcca ccatcgcgac cacgcaccag ttctgccaga tcgtgctgaa atctcttggc gtggctgggg actccgacgc cggggtgacc ctcgtggaga gcctcgacga cttggtctcc gagatcgtcg acgatctgta tctcgcgcac ttcggcgggc agaaggacga tcccgagctc agctatcccg aggccctcaa gctcgcgcgc gtcgtcgtcg gcaacccggc cacccaactg cgcccgcgcg acccggaccc cgattccccc gccgcggtgc gcctgaagtt cgcgcgggat gtgctcgccg agttggagat ccgcaagcgt cggcgcggcg tgctcggcta cgacgatctg ctgacccggc tggccgatgc actcgaaccc gaggactccc cggcgcgcgt gcgcatgcag cagcgctggc cgatcgtgat ggtcgacgag ttccaggaca ccgatcccgt gcagtggcag gtgatcgagc gcgcgttctc ggggcggtcg acgctggtgc tcatcggtga tcccaagcag gcgatctacg cgttccgcgg tggcgacatc gcgacgtacc tgcgggccgc cgcgacggcc ggtgacaagc agacgctggg caccaactgg cgcagcgacc gcgcactggt ggaccgcctg caggccgtgc tgcgcggcgc acaactcggc ggacccgaca tcgtggtgca cgacgtgcag gcacgccatc aggggcaccg cctggtgggc gcaccgcgca acgacccgtt ccggctgcgc gtggtgtcac gaaaacccgg caacaccagg gtgatcccga tcgatcagct gcgccgccac atcggccggg atctcgcggc cgacatctct gccctgctga acagcggggc cacgtggtgc gaccaaccgg tccaggcgaa ggacatcgcg gtcatcaccg agacccacaa ggacgccagg gcgtgtcatg cggcgctgct cgcggcgggc attcccgcgg tctacaccgg ggattcggat gtgttcacct ccgaggccgc cgaggactgg ctgtacctgc tcgaggcgtt cgaccagccg caccggccgg gtctggtgcg cgccgcggcc gccaccatgt tcttcggcga gaccgcggaa agccttgccg cgggcggcga tgcgctcacc gaccgggtcg ccgacacgct gcgtgaatgg gcggggcatg cccgtgagcg cggtgtcgcc gcgatcttcg aggccgcgca actcgccggt atgggcaagc gtgtgctgtc gtggcagggc ggtgaacggt tgatgacgga tctcgcccac atgacccagt tgctgcagga caccgcacac cgcgagggct tcgggctggc cgcgctgcgc gactggctgc gcacgcagcg ctcggagagg ggcggcgaat cggaacgcaa ccgtcgtctc gacagtgacg ccgcggccgt gcagatcatg accgtgtggg tgagcaaggg tctgcagttc ccggtcgtct acctgccgtt cgcgttcaat cgctatgtgc ccgagccgga tctggtgttg ttccacgacg acgggcagcg ctgcctgcat gtcggcggag ccgatccggc ggtcgcccgc gcggggcgtg ccgaggccgc gggcgacgac agccgtctca cctacgtcgc actgacccgc gcccagtcgc aggtggtggc gtggtgggca ccgtcgtacg acgaacccaa cggcggcctg tcgcggctga tgcgcggccg cgctcccggg gaggcgatcg tccccgacaa gtgctcacca cccaagatct ccgatgagga tgcgctggaa cggttgcggg cgtgggaggc agccggtggg ccggtgatcg aggaatcggt gatcggtgcg gtcagcccgg tgccacccga gcccgcgccc gaggatctgg cggctcgcaa gttctttcgc gcgatcgaca tggcgtggaa gcgcacgtcc tattcggggc tcttgcgcgc ggcagagacc gcgggtgtcg gggtgtcgag cgaacccgag gtcaccgaac gcgacgacga gttcgacgac attcccgtgg tcgccccggc cgagggtgcc gacgtgccct ctcccatggc acacctgccg acgggcgcgg cgttcggttc gctcgtgcac gccgtgctgg agaccgccga tccgttcgcc gaggatctga ccgccgaact cgcgacgcac atcgacgccc actcccagca ctggccggtc gaggtcgaga cggccgaact cgcggccgcg ctcgtgccca tgcacgacac cccgctgggg ccgttggcgc cgggtctcac gctgcggcag atcggtctac gggaccggtt gtgtgagttg gatttcgagt ttcccatggc cggtggcgat ctgcgtggtg gacggttcgc acggttgtcg gacgtcggtg agctgctgcg tgagtacctg cccgccgacg acccgctggc ggtgtacgcc gagcggttgt ccacgggcat cctcggggtg cagccgctgc gcgggtatct gtcggggtcg gtcgacgcgg tgctgcgggt cggcgagaag ttcgtcatcg tcgactacaa gacgaactgg ctgggcaccg gcgacggcac gttgaccgcg gccgactacg ggcgccgccg catggtcgag gccatgttgc attccgacta tccgctgcag gccctgctgt atgcggttgt gctgcaccgg tacctgggct ggcggctgtc gggctacgac ccggcgacgc atctcggtgg tgtgctgtat ctgttcgtac gtggcatgtg cggggccggg acgccggtcg tcgacgggca cccagccggg gtgttcagct gggaaccgcc tgccgatctg gtcgtcgcat tgtcgaagct gttggatgcg gaggcgccgt ga

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