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MytuD.00010.a: 3-ketoacyl-(acyl-carrier-protein) reductase

Target Characteristics

from organism: Mycobacterium tuberculosis H37Rv
most recent status: in PDB
center reference id: MytuD.00010.a
is community request: False
associated disease: Tuberculosis
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00010.a.A1.GE25247 Full length( MytuD.00010.a ) 1 454 GE25247
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

image of structure for 3LLS
3LLS
Deposited: 1/29/2010
Determination: XRay
Diffraction Image
Clone: MytuD.00010.a.A1.GE25247
Protein: MytuD.00010.a.A1.PW25285

Publications by SSGCID

Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB: 3GVC, 3GVG, 3GWC, 3H7F, 3H81, 3HE2, 3HWI, 3HWK, 3HZG, 3ICO, 3KHP, 3LLS, 3MOY, 3MPZ, 3MYB, 3NDN, 3NDO, 3NF4, 3NG3, 3NJD, 3NWO, 3O0M, 3O38, 3OC6, 3OC7, 3OEC, 3OI9, 3OKS, 3OL3, 3OL4, 3OME, 3P0T, 3P2Y, 3P4I, 3P4T, 3P5M, 3P85, 3PE8, 3PGX, 3PK0, 3PPI, 3PXX, 3PZY, 3Q1T, 3Q8N, 3QBP, 3QDF, 3QH4, 3QHA, 3QIV, 3QK8, 3QKA, 3QLJ, 3QMJ, 3QRE, 3QUA, 3QUV, 3QXI, 3QXZ, 3QYR, 3R0O, 3R1I, 3R1J, 3R20, 3R2N, 3R4T, 3R6H, 3R6O, 3R7K, 3R8C, 3R9P, 3R9Q, 3R9R, 3R9S, 3R9T, 3RD5, 3RD7, 3RD8, 3RFQ, 3RIH, 3RR2, 3RR6, 3RRP, 3RRV, 3RSI, 3RV2, 3S55, 3S82, 3SBX, 3SF6, 3SLL, 3SVK, 3SVT, 3SWO, 3SWT, 3SWX, 3SX2, 3T3W, 3T7C, 3TAV, 3TCR, 3TDE, 3TJR, 3TL3, 3TLF, 3TRR, 3TSC, 3TX2, 3TZQ, 3TZU, 3U0A, 3U0B, 3UCX, 3UVE, 4DI1, 4DIE, 4DQ8, 4DXL, 4ED4, 4EGE, 4EGF, 4EMD, 4EO9, 4EYE, 4F3W, 4F47, 4FFC, 4GK6, 4HDT, 4HR3, 4I1Y, 4IJN, 4IV6, 4IZ9, 4J5I, 4KAM, 4LGV, 4O2D, 5EJ2

Citations by Others

PEPTIDOMIMETIC COMPOUNDS
RT Skerlj, AC Good - US Patent 20,150,353,606, 2015 - freepatentsonline.com
PDB: 3LLS
Crystal structure of hexanoyl-CoA bound to beta-ketoacyl reductase FabG4 of Mycobacterium tuberculosis
D Debajyoti, B Sudipta, R Amlan, B Rupam? - Biochemical Journal, 2013 - biochemj.org
PDB: 3LLS
Crystallization and preliminary X-ray diffraction analysis of the high molecular weight ketoacyl reductase FabG4 complexed with NADH
D Dutta, S Bhattacharyya, AK Das - Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2012 - scripts.iucr.org
cited by: 3 others
PDB: 3LLS
Crystal structure of FabG4 from< i> Mycobacterium tuberculosis reveals the importance of C-terminal residues in ketoreductase activity
D Dutta, S Bhattacharyya, S Mukherjee, B Saha? - Journal of Structural Biology, 2011 - Elsevier
cited by: 10 others
PDB: 3LLS

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_187141
PATRIC ID: fig|83332.12.peg.272
RefSeq: NP_214756.1
TubercuList: Rv0242c
MTB Network Portal: Rv0242c
UniProt: I6Y778

Orthologues for Rv0242c

Species % Identity % Coverage Clones Proteins Structures
avium 90 100 0 0 0
paratuberculosis 90 100 1 0 0
marinum 88 100 0 0 0
ulcerans 87 100 1 0 0
leprae 85 100 0 0 0
smegmatis 84 99 1 0 2
thermoresistibile 81 52 0 0 0
abscessus 78 100 0 0 0

Enzyme & Pathway Information

Pathway Pathway ID EC Number
biotin biosynthesis I BIOTIN-BIOSYNTHESIS-PWY 1.1.1.100
(5Z)-dodecenoate biosynthesis I PWY0-862 1.1.1.100
superpathway of fatty acid biosynthesis I (E. coli) PWY0-881 1.1.1.100
arachidonate biosynthesis PWY-5353 1.1.1.100
petroselinate biosynthesis PWY-5367 1.1.1.100
cis-vaccenate biosynthesis PWY-5973 1.1.1.100
superpathway of mycolate biosynthesis PWY-6113 1.1.1.100
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) PWY-6282 1.1.1.100
superpathway of unsaturated fatty acids biosynthesis (E. coli) PWY-6284 1.1.1.100
superpathway of fatty acids biosynthesis (E. coli) PWY-6285 1.1.1.100
8-amino-7-oxononanoate biosynthesis I PWY-6519 1.1.1.100
eicosapentaenoate biosynthesis from arachidonate PWY-6940 1.1.1.100
docosahexanoate biosynthesis II PWY-6951 1.1.1.100
docosahexanoate biosynthesis I PWY-7053 1.1.1.100
gondoate biosynthesis (anaerobic) PWY-7663 1.1.1.100
oleate biosynthesis IV (anaerobic) PWY-7664 1.1.1.100
(5Z)-dodecenoate biosynthesis II PWY-7858 1.1.1.100
mycolate biosynthesis PWYG-321 1.1.1.100

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MAPKRSSDLF SQVVNSGPGS FLARQLGVPQ PETLRRYRAG EPPLTGSLLI GGAGRVVEPL RAALEKDYDL VGNNLGGRWA DSFGGLVFDA TGITEPAGLK GLHEFFTPVL RNLGRCGRVV VVGGTPEAAA STNERIAQRA LEGFTRSLGK ELRRGATTAL VYLSPDAKPA ATGLESTMRF LLSAKSAYVD GQVFSVGADD STPPADWEKP LDGKVAIVTG AARGIGATIA EVFARDGAHV VAIDVESAAE NLAETASKVG GTALWLDVTA DDAVDKISEH LRDHHGGKAD ILVNNAGITR DKLLANMDDA RWDAVLAVNL LAPLRLTEGL VGNGSIGEGG RVIGLSSIAG IAGNRGQTNY ATTKAGMIGI TQALAPGLAA KGITINAVAP GFIETQMTAA IPLATREVGR RLNSLLQGGQ PVDVAEAIAY FASPASNAVT GNVIRVCGQA MIGA

NT Sequence
GTGGCTCCCA AGCGTTCGTC CGATCTGTTC TCGCAGGTTG TCAACTCCGG TCCAGGATCG TTTTTGGCCA GGCAATTGGG TGTTCCGCAA CCCGAGACGC TTCGCCGCTA TCGAGCAGGC GAACCGCCAC TGACCGGGTC GCTGCTGATC GGCGGGGCCG GCAGGGTCGT CGAACCACTG CGCGCGGCAC TCGAAAAGGA CTACGACCTG GTAGGAAACA ACCTGGGCGG GCGCTGGGCG GACTCGTTCG GCGGGCTGGT CTTCGACGCC ACCGGCATCA CAGAGCCGGC CGGGCTCAAG GGGCTGCACG AGTTCTTTAC TCCGGTACTG CGCAATCTCG GCCGTTGCGG GCGCGTGGTG GTTGTTGGCG GCACACCCGA AGCGGCCGCC AGCACGAACG AGCGGATCGC GCAGCGCGCG CTGGAGGGCT TCACCCGCTC GCTGGGCAAG GAACTGCGCC GCGGTGCGAC AACGGCGTTG GTCTACCTGT CGCCGGACGC CAAGCCCGCA GCTACCGGCC TCGAGTCGAC CATGCGATTC CTGCTGTCGG CCAAGTCGGC CTACGTCGAC GGCCAAGTCT TCTCTGTCGG GGCGGACGAT TCCACACCGC CGGCCGACTG GGAGAAGCCG CTGGACGGCA AGGTCGCCAT TGTGACCGGC GCGGCCCGCG GCATCGGCGC AACCATCGCC GAGGTGTTCG CTCGCGACGG CGCCCACGTG GTCGCCATCG ATGTGGAGTC CGCCGCCGAG AACCTGGCCG AAACCGCCAG CAAGGTGGGC GGCACCGCGC TGTGGCTCGA CGTCACCGCC GACGATGCCG TCGACAAGAT CAGCGAGCAC CTGCGCGACC ACCACGGTGG CAAGGCCGAC ATCCTGGTCA ACAACGCCGG CATCACCCGT GACAAGCTGC TGGCCAACAT GGACGATGCG CGTTGGGACG CCGTCCTGGC TGTCAATCTG CTTGCCCCGT TGCGGCTCAC CGAGGGTCTG GTGGGCAACG GCAGCATCGG AGAGGGTGGT CGGGTGATCG GGCTGTCGTC GATCGCCGGC ATCGCCGGCA ACCGCGGCCA GACCAACTAC GCCACCACCA AGGCCGGGAT GATCGGCATC ACCCAGGCGC TGGCACCCGG CCTGGCCGCG AAAGGCATCA CGATCAACGC CGTGGCACCG GGATTCATCG AAACCCAGAT GACGGCCGCC ATCCCGCTGG CCACCCGTGA GGTAGGCCGC CGGCTGAACT CGCTGCTGCA GGGCGGGCAG CCCGTCGACG TCGCCGAGGC CATCGCCTAC TTCGCCAGCC CGGCGTCAAA CGCGGTGACC GGCAACGTCA TTCGTGTCTG CGGCCAGGCC ATGATCGGCG CG

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