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Seattle Structural Genomics Center for Infectious Disease

MytuD.00213.a: Enoyl-CoA hydratase

Target Characteristics

from organism: Mycobacterium tuberculosis H37Rv
most recent status: purified
center reference id: MytuD.00213.a
is community request: False
associated disease: Tuberculosis
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00213.a.A1.GE25815 Full length( MytuD.00213.a ) 1 274 GE25815
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

Resource Reference ID
OrthoMCL: OG5_129459
PATRIC ID: fig|83332.12.peg.4205
RefSeq: NP_218291.1
TubercuList: Rv3774
MTB Network Portal: Rv3774
UniProt: P75019

Orthologues for Rv3774

Species % Identity % Coverage Clones Proteins Structures
marinum 90 100 1 1 0
leprae 88 100 0 0 0
avium 84 100 1 1 0
paratuberculosis 84 100 1 1 0
smegmatis 80 100 2 1 0
abscessus 68 98 3 1 0

Enzyme & Pathway Information

Pathway Pathway ID EC Number
fatty acid beta-oxidation I FAO-PWY 4.2.1.17
L-isoleucine degradation I ILEUDEG-PWY 4.2.1.17
oleate beta-oxidation PWY0-1337 4.2.1.17
phenylacetate degradation I (aerobic) PWY0-321 4.2.1.17
benzoyl-CoA degradation I (aerobic) PWY-1361 4.2.1.17
fermentation to 2-methylbutanoate PWY-5109 4.2.1.17
fatty acid beta-oxidation II (peroxisome) PWY-5136 4.2.1.17
unsaturated, even numbered fatty acid beta-oxidation PWY-5138 4.2.1.17
superpathway of glyoxylate cycle and fatty acid degradation PWY-561 4.2.1.17
superpathway of phenylethylamine degradation PWY-6071 4.2.1.17
4-hydroxybenzoate biosynthesis III (plants) PWY-6435 4.2.1.17
fatty acid beta-oxidation VI (peroxisome) PWY66-391 4.2.1.17
pyruvate fermentation to hexanol (engineered) PWY-6863 4.2.1.17
superpathway of cholesterol degradation I (cholesterol oxidase) PWY-6928 4.2.1.17
superpathway of testosterone and androsterone degradation PWY-6937 4.2.1.17
androstenedione degradation PWY-6944 4.2.1.17
superpathway of cholesterol degradation II (cholesterol dehydrogenase) PWY-6947 4.2.1.17
methyl ketone biosynthesis (engineered) PWY-7007 4.2.1.17
4-coumarate degradation (anaerobic) PWY-7046 4.2.1.17
fatty acid salvage PWY-7094 4.2.1.17
jasmonic acid biosynthesis PWY-735 4.2.1.17
docosahexaenoate biosynthesis III (6-desaturase, mammals) PWY-7606 4.2.1.17
(8E,10E)-dodeca-8,10-dienol biosynthesis PWY-7654 4.2.1.17
Spodoptera littoralis pheromone biosynthesis PWY-7656 4.2.1.17
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) PWY-7726 4.2.1.17
toluene degradation to benzoyl-CoA (anaerobic) PWY-81 4.2.1.17
L-valine degradation I VALDEG-PWY 4.2.1.17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MGETYESVTV ETKDQVAQVT LIGPGKGNAM GPAFWSEMPE VFHALDADRE VRAIVITGSG KNFSYGLDVP AMGGMFAPLI ADGALARPRT DFHTEILRMQ KAINAVADCR TPTIAAVQGW CIGGAVDLIS AVDIRYASAD AKFSVREVKL AIVADMGSLA RLPLILSDGH LRELALTGKN IDAARAEKIG LVNDVYDDAD QTLAAAHATA AEIAANPPLA VYGIKDVLDQ QRTSAVSENL RYVAAWNAAF LPSKDLTEGI SATFAKRPPQ FTGE

NT Sequence
ATGGGCGAAA CCTACGAATC CGTCACCGTC GAAACCAAGG ACCAGGTCGC GCAGGTGACG CTGATCGGGC CGGGCAAGGG CAACGCGATG GGGCCCGCAT TCTGGTCGGA GATGCCCGAG GTGTTCCATG CCCTGGACGC CGACCGTGAG GTGCGGGCCA TCGTCATCAC CGGATCGGGC AAGAACTTCA GCTACGGCCT GGACGTACCG GCCATGGGCG GAATGTTCGC CCCGTTGATC GCCGACGGCG CGCTGGCCCG CCCACGCACG GACTTCCACA CCGAAATACT GCGCATGCAG AAGGCGATCA ACGCCGTCGC CGACTGCCGC ACCCCCACGA TCGCGGCCGT CCAGGGTTGG TGCATCGGCG GCGCCGTCGA CCTGATCTCC GCGGTCGACA TCCGGTATGC CAGCGCCGAC GCGAAGTTCT CGGTGCGCGA GGTCAAGCTA GCGATTGTTG CCGACATGGG CAGCCTGGCG CGCCTTCCAC TAATCCTGAG CGACGGCCAT CTACGAGAAC TCGCGCTGAC CGGCAAAAAT ATCGACGCGG CCCGCGCCGA GAAGATCGGC CTGGTCAACG ACGTCTACGA TGACGCCGAC CAGACGCTGG CCGCGGCCCA CGCGACTGCC GCCGAGATCG CCGCCAACCC ACCTTTGGCG GTCTACGGCA TCAAGGACGT TCTCGACCAA CAACGCACGT CCGCCGTCTC GGAGAACCTG CGCTATGTCG CCGCCTGGAA CGCCGCGTTT CTGCCGTCCA AGGACCTCAC CGAAGGTATT TCCGCGACGT TCGCCAAGCG CCCGCCCCAG TTCACCGGCG AG

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