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Seattle Structural Genomics Center for Infectious Disease

MytuD.00698.a: methylcitrate synthase

Target Characteristics

from organism: Mycobacterium tuberculosis H37Rv
most recent status: in PDB
center reference id: MytuD.00698.a
is community request: False
associated disease: Tuberculosis
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00698.a.A1.GE25873 Full length( MytuD.00698.a ) 1 393 GE25873
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

image of structure for 3HWK
3HWK
Deposited: 6/17/2009
Determination: XRay
Diffraction Image
Clone: MytuD.00698.a.A1.GE25873
Protein: MytuD.00698.a.A1.PW25932

Publications by SSGCID

Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB: 3GVC, 3GVG, 3GWC, 3H7F, 3H81, 3HE2, 3HWI, 3HWK, 3HZG, 3ICO, 3KHP, 3LLS, 3MOY, 3MPZ, 3MYB, 3NDN, 3NDO, 3NF4, 3NG3, 3NJD, 3NWO, 3O0M, 3O38, 3OC6, 3OC7, 3OEC, 3OI9, 3OKS, 3OL3, 3OL4, 3OME, 3P0T, 3P2Y, 3P4I, 3P4T, 3P5M, 3P85, 3PE8, 3PGX, 3PK0, 3PPI, 3PXX, 3PZY, 3Q1T, 3Q8N, 3QBP, 3QDF, 3QH4, 3QHA, 3QIV, 3QK8, 3QKA, 3QLJ, 3QMJ, 3QRE, 3QUA, 3QUV, 3QXI, 3QXZ, 3QYR, 3R0O, 3R1I, 3R1J, 3R20, 3R2N, 3R4T, 3R6H, 3R6O, 3R7K, 3R8C, 3R9P, 3R9Q, 3R9R, 3R9S, 3R9T, 3RD5, 3RD7, 3RD8, 3RFQ, 3RIH, 3RR2, 3RR6, 3RRP, 3RRV, 3RSI, 3RV2, 3S55, 3S82, 3SBX, 3SF6, 3SLL, 3SVK, 3SVT, 3SWO, 3SWT, 3SWX, 3SX2, 3T3W, 3T7C, 3TAV, 3TCR, 3TDE, 3TJR, 3TL3, 3TLF, 3TRR, 3TSC, 3TX2, 3TZQ, 3TZU, 3U0A, 3U0B, 3UCX, 3UVE, 4DI1, 4DIE, 4DQ8, 4DXL, 4ED4, 4EGE, 4EGF, 4EMD, 4EO9, 4EYE, 4F3W, 4F47, 4FFC, 4GK6, 4HDT, 4HR3, 4I1Y, 4IJN, 4IV6, 4IZ9, 4J5I, 4KAM, 4LGV, 4O2D, 5EJ2

Citations by Others

Crystal structure of< i> Salmonella typhimurium 2-methylcitrate synthase: Insights on domain movement and substrate specificity
S Chittori, HS Savithri, MRN Murthy - Journal of Structural Biology, 2011 - Elsevier
cited by: 1 other
PDB: 3HWK

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_128590
PATRIC ID: fig|83332.12.peg.1267
RefSeq: NP_215647.1
TubercuList: Rv1131
MTB Network Portal: Rv1131
UniProt: I6Y9Q3

Orthologues for Rv1131

Species % Identity % Coverage Clones Proteins Structures
smegmatis 80 95 0 1 0
marinum 80 94 0 0 0
ulcerans 80 94 0 0 0
abscessus 80 93 0 0 0
avium 80 93 0 0 0
paratuberculosis 80 93 0 0 0

Enzyme & Pathway Information

Pathway Pathway ID EC Number
respiration (anaerobic) ANARESP1-PWY 2.3.3.1
mixed acid fermentation FERMENTATION-PWY 2.3.3.16
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass GLYCOLYSIS-TCA-GLYOX-BYPASS 2.3.3.16
glyoxylate cycle GLYOXYLATE-BYPASS 2.3.3.16
TCA cycle IV (2-oxoglutarate decarboxylase) P105-PWY 2.3.3.16
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle PWY-5464 2.3.3.16
superpathway of glyoxylate cycle and fatty acid degradation PWY-561 2.3.3.16
TCA cycle II (plants and fungi) PWY-5690 2.3.3.16
partial TCA cycle (obligate autotrophs) PWY-5913 2.3.3.16
L-glutamine biosynthesis III PWY-6549 2.3.3.16
TCA cycle III (animals) PWY66-398 2.3.3.16
methylaspartate cycle PWY-6728 2.3.3.16
TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase) PWY-6969 2.3.3.16
ethylene biosynthesis V (engineered) PWY-7124 2.3.3.16
TCA cycle VII (acetate-producers) PWY-7254 2.3.3.16
TCA cycle VIII (helicobacter) REDCITCYC 2.3.3.16
TCA cycle I (prokaryotic) TCA 2.3.3.16
superpathway of glyoxylate bypass and TCA TCA-GLYOX-BYPASS 2.3.3.16
2-methylcitrate cycle I PWY0-42 2.3.3.5
2-methylcitrate cycle II PWY-5747 2.3.3.5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MTGPLAAARS VAATKSMTAP TVDERPDIKK GLAGVVVDTT AISKVVPQTN SLTYRGYPVQ DLAARCSFEQ VAFLLWRGEL PTDAELALFS QRERASRRVD RSMLSLLAKL PDNCHPMDVV RTAISYLGAE DPDEDDAAAN RAKAMRMMAV LPTIVAIDMR RRRGLPPIAP HSGLGYAQNF LHMCFGEVPE TAVVSAFEQS MILYAEHGFN ASTFAARVVT STQSDIYSAV TGAIGALKGR LHGGANEAVM HDMIEIGDPA NAREWLRAKL ARKEKIMGFG HRVYRHGDSR VPTMKRALER VGTVRDGQRW LDIYQVLAAE MASATGILPN LDFPTGPAYY LMGFDIASFT PIFVMSRITG WTAHIMEQAT ANALIRPLSA YCGHEQRVLP GTF

NT Sequence
ATGACCGGGC CGCTCGCGGC GGCCAGGTCC GTCGCTGCCA CGAAATCGAT GACCGCGCCC ACCGTTGATG AGCGGCCCGA CATCAAAAAG GGCCTCGCCG GCGTGGTGGT GGACACCACC GCCATCTCCA AGGTGGTGCC GCAGACCAAT TCGTTGACCT ACCGGGGATA TCCGGTCCAG GATCTGGCAG CCCGCTGCAG TTTCGAGCAG GTCGCCTTCC TGCTGTGGCG TGGTGAGTTG CCCACCGATG CCGAGCTGGC GTTGTTCAGC CAGCGCGAAC GAGCCAGCCG TCGGGTGGAC CGCTCGATGC TGTCATTGCT GGCCAAGCTG CCGGACAACT GCCACCCGAT GGACGTGGTG CGCACCGCGA TCAGCTATCT CGGTGCCGAG GACCCGGACG AGGACGACGC CGCGGCCAAC CGGGCCAAGG CGATGCGCAT GATGGCGGTG TTGCCGACGA TCGTGGCGAT CGACATGCGG CGCCGACGCG GGTTGCCCCC GATCGCACCG CACAGCGGGC TCGGTTATGC GCAGAACTTC CTGCACATGT GCTTCGGGGA GGTACCCGAA ACCGCCGTCG TGTCGGCGTT CGAGCAGTCG ATGATCCTCT ACGCCGAGCA CGGATTCAAC GCGTCGACGT TCGCCGCCCG GGTGGTGACC TCGACCCAAT CCGACATCTA CAGCGCGGTG ACCGGCGCGA TCGGCGCCCT CAAGGGGCGG CTACACGGCG GCGCCAACGA AGCCGTCATG CACGACATGA TCGAGATCGG CGATCCGGCC AACGCGCGGG AGTGGTTGCG CGCCAAGCTC GCCCGCAAGG AAAAGATCAT GGGCTTCGGG CATCGGGTGT ACCGGCACGG CGACTCCCGG GTGCCGACCA TGAAACGGGC GCTGGAGCGC GTGGGGACCG TTCGCGACGG CCAGCGATGG CTGGACATCT ACCAGGTGTT AGCGGCCGAG ATGGCGTCGG CCACCGGGAT CTTGCCCAAC CTCGATTTTC CGACCGGGCC CGCGTACTAC CTGATGGGAT TCGACATCGC CAGCTTCACC CCGATCTTCG TGATGAGTAG GATCACCGGC TGGACCGCAC ACATCATGGA ACAGGCCACG GCCAACGCGC TGATCCGGCC GCTGAGCGCA TATTGCGGGC ACGAGCAGCG GGTGTTACCG GGCACCTTC

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