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Seattle Structural Genomics Center for Infectious Disease

MytuD.01530.a: Enoyl-CoA hydratase

Target Characteristics

from organism: Mycobacterium tuberculosis H37Rv
most recent status: purified
center reference id: MytuD.01530.a
is community request: False
associated disease: Tuberculosis
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.01530.a.A1.GE25970 Full length( MytuD.01530.a ) 1 263 GE25970
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.01530.a.A1.PS00181 Full length( MytuD.01530.a ) 1 263

External Resources

Resource Reference ID
OrthoMCL: OG5_169926
PATRIC ID: fig|83332.12.peg.3926
RefSeq: NP_218033.1
TubercuList: Rv3516
MTB Network Portal: Rv3516
UniProt: O53561

Orthologues for Rv3516

Species % Identity % Coverage Clones Proteins Structures
paratuberculosis 92 100 1 0 0
marinum 91 100 1 1 1
ulcerans 90 100 1 1 0
smegmatis 84 99 1 2 0
abscessus 79 98 0 0 0
abscessus 48 100 1 1 1
smegmatis 35 97 1 1 0
marinum 35 96 1 0 0
marinum 34 97 0 0 0
ulcerans 34 97 1 0 0
ulcerans 34 96 0 0 0
paratuberculosis 34 95 1 1 1
smegmatis 33 96 0 0 0
abscessus 32 97 1 1 1
avium 31 80 1 2 0
thermoresistibile 30 95 1 1 0
abscessus 28 95 1 1 0

Enzyme & Pathway Information

Pathway Pathway ID EC Number
fatty acid beta-oxidation I FAO-PWY 4.2.1.17
L-isoleucine degradation I ILEUDEG-PWY 4.2.1.17
oleate beta-oxidation PWY0-1337 4.2.1.17
phenylacetate degradation I (aerobic) PWY0-321 4.2.1.17
benzoyl-CoA degradation I (aerobic) PWY-1361 4.2.1.17
fermentation to 2-methylbutanoate PWY-5109 4.2.1.17
fatty acid beta-oxidation II (peroxisome) PWY-5136 4.2.1.17
unsaturated, even numbered fatty acid beta-oxidation PWY-5138 4.2.1.17
superpathway of glyoxylate cycle and fatty acid degradation PWY-561 4.2.1.17
superpathway of phenylethylamine degradation PWY-6071 4.2.1.17
4-hydroxybenzoate biosynthesis III (plants) PWY-6435 4.2.1.17
fatty acid beta-oxidation VI (peroxisome) PWY66-391 4.2.1.17
pyruvate fermentation to hexanol (engineered) PWY-6863 4.2.1.17
superpathway of cholesterol degradation I (cholesterol oxidase) PWY-6928 4.2.1.17
superpathway of testosterone and androsterone degradation PWY-6937 4.2.1.17
androstenedione degradation PWY-6944 4.2.1.17
superpathway of cholesterol degradation II (cholesterol dehydrogenase) PWY-6947 4.2.1.17
methyl ketone biosynthesis (engineered) PWY-7007 4.2.1.17
4-coumarate degradation (anaerobic) PWY-7046 4.2.1.17
fatty acid salvage PWY-7094 4.2.1.17
jasmonic acid biosynthesis PWY-735 4.2.1.17
docosahexaenoate biosynthesis III (6-desaturase, mammals) PWY-7606 4.2.1.17
(8E,10E)-dodeca-8,10-dienol biosynthesis PWY-7654 4.2.1.17
Spodoptera littoralis pheromone biosynthesis PWY-7656 4.2.1.17
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) PWY-7726 4.2.1.17
toluene degradation to benzoyl-CoA (anaerobic) PWY-81 4.2.1.17
L-valine degradation I VALDEG-PWY 4.2.1.17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MESGPDALVE RRGHTLIVTM NRPAARNALS TEMMRIMVQA WDRVDNDPDI RCCILTGAGG YFCAGMDLKA ATQKPPGDSF KDGSYGPSRI DALLKGRRLT KPLIAAVEGP AIAGGTEILQ GTDIRVAGES AKFGISEAKW SLYPMGGSAV RLVRQIPYTL ACDLLLTGRH ITAAEAKEMG LIGHVVPDGQ ALTKALELAD AISANGPLAV QAILRSIRET ECMPENEAFK IDTQIGIKVF LSDDAKEGPR AFAEKRAPNF QNR

NT Sequence
GTGGAATCCG GACCCGACGC GCTGGTGGAG CGGCGCGGCC ACACCCTGAT CGTGACCATG AACCGGCCGG CCGCCCGCAA CGCGCTGAGC ACCGAAATGA TGCGAATCAT GGTGCAGGCC TGGGATCGCG TCGACAACGA TCCCGACATC CGTTGCTGCA TCCTCACCGG AGCCGGTGGC TACTTTTGCG CCGGCATGGA CCTCAAGGCG GCAACCCAGA AACCGCCGGG CGACTCTTTC AAGGACGGCA GCTACGGCCC GTCGCGCATC GATGCCCTGC TCAAAGGGCG CCGCTTGACC AAACCGCTGA TCGCCGCCGT CGAGGGGCCC GCGATCGCCG GCGGCACCGA GATCCTGCAG GGCACCGACA TCCGGGTCGC CGGTGAAAGT GCGAAGTTCG GCATCTCCGA GGCCAAGTGG AGCCTGTACC CGATGGGCGG CTCGGCCGTG CGGCTGGTCC GGCAGATCCC CTACACTCTG GCCTGCGACC TGCTGCTGAC CGGACGGCAC ATTACCGCCG CCGAGGCCAA GGAAATGGGC CTGATCGGCC ACGTGGTGCC CGACGGCCAG GCGCTGACCA AGGCTCTAGA ACTTGCCGAC GCCATCTCGG CTAACGGACC CCTGGCCGTG CAGGCCATCC TGCGGTCCAT CCGCGAGACC GAGTGCATGC CCGAAAACGA GGCGTTCAAG ATCGACACCC AGATCGGCAT CAAGGTCTTC CTGTCCGACG ACGCCAAGGA AGGCCCGCGC GCGTTCGCCG AGAAGCGCGC ACCCAACTTC CAGAACCGC

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