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Seattle Structural Genomics Center for Infectious Disease

MytuD.01625.a: S-adenosylmethionine synthetase

Target Characteristics

from organism: Mycobacterium tuberculosis H37Rv
most recent status: in PDB
center reference id: MytuD.01625.a
is community request: False
associated disease: Tuberculosis
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.01625.a.A1.GE28467 Full length( MytuD.01625.a ) 1 403 GE28467
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

image of structure for 3TDE
3TDE
Deposited: 8/10/2011
Determination: XRay
Diffraction Image
Clone: MytuD.01625.a.A1.GE28467
Protein: MytuD.01625.a.A1.PS00654

Publications by SSGCID

Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB: 3GVC, 3GVG, 3GWC, 3H7F, 3H81, 3HE2, 3HWI, 3HWK, 3HZG, 3ICO, 3KHP, 3LLS, 3MOY, 3MPZ, 3MYB, 3NDN, 3NDO, 3NF4, 3NG3, 3NJD, 3NWO, 3O0M, 3O38, 3OC6, 3OC7, 3OEC, 3OI9, 3OKS, 3OL3, 3OL4, 3OME, 3P0T, 3P2Y, 3P4I, 3P4T, 3P5M, 3P85, 3PE8, 3PGX, 3PK0, 3PPI, 3PXX, 3PZY, 3Q1T, 3Q8N, 3QBP, 3QDF, 3QH4, 3QHA, 3QIV, 3QK8, 3QKA, 3QLJ, 3QMJ, 3QRE, 3QUA, 3QUV, 3QXI, 3QXZ, 3QYR, 3R0O, 3R1I, 3R1J, 3R20, 3R2N, 3R4T, 3R6H, 3R6O, 3R7K, 3R8C, 3R9P, 3R9Q, 3R9R, 3R9S, 3R9T, 3RD5, 3RD7, 3RD8, 3RFQ, 3RIH, 3RR2, 3RR6, 3RRP, 3RRV, 3RSI, 3RV2, 3S55, 3S82, 3SBX, 3SF6, 3SLL, 3SVK, 3SVT, 3SWO, 3SWT, 3SWX, 3SX2, 3T3W, 3T7C, 3TAV, 3TCR, 3TDE, 3TJR, 3TL3, 3TLF, 3TRR, 3TSC, 3TX2, 3TZQ, 3TZU, 3U0A, 3U0B, 3UCX, 3UVE, 4DI1, 4DIE, 4DQ8, 4DXL, 4ED4, 4EGE, 4EGF, 4EMD, 4EO9, 4EYE, 4F3W, 4F47, 4FFC, 4GK6, 4HDT, 4HR3, 4I1Y, 4IJN, 4IV6, 4IZ9, 4J5I, 4KAM, 4LGV, 4O2D, 5EJ2

Citations by Others

The interdimeric interface controls function and stability of Ureaplasma urealiticum methionine S-adenosyltransferase
D Kleiner, F Shmulevich, R Zarivach, A Shahar- Journal of molecular, 2019 - Elsevier
cited by: 4 others
PDB: 3TDE
Synthesis of non-natural cofactor analogs of S-adenosyl-L-methionine using methionine adenosyltransferase
J Thorson, T Huber, J Zhang, S Singh- US Patent 9,879,043, 2018 - freepatentsonline.com
PDB: 3TDE

This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.

External Resources

Resource Reference ID
OrthoMCL: OG5_126734
PATRIC ID: fig|83332.12.peg.1557
RefSeq: NP_215908.1
TubercuList: Rv1392
MTB Network Portal: Rv1392
UniProt: P9WGV1

Orthologues for Rv1392

Species % Identity % Coverage Clones Proteins Structures
marinum 95 100 1 0 1
ulcerans 95 100 1 0 0
avium 92 100 1 0 1
leprae 92 100 1 0 0
paratuberculosis 92 100 0 0 0
smegmatis 87 99 0 1 0
abscessus 85 97 1 0 0

Enzyme & Pathway Information

Pathway Pathway ID EC Number
ethylene biosynthesis I (plants) ETHYL-PWY 2.5.1.6
L-methionine degradation I (to L-homocysteine) METHIONINE-DEG1-PWY 2.5.1.6
superpathway of S-adenosyl-L-methionine biosynthesis MET-SAM-PWY 2.5.1.6
aspartate superpathway PWY0-781 2.5.1.6
S-adenosyl-L-methionine cycle II PWY-5041 2.5.1.6
superpathway of L-methionine salvage and degradation PWY-5328 2.5.1.6
2'-deoxymugineic acid phytosiderophore biosynthesis PWY-5912 2.5.1.6
S-adenosyl-L-methionine cycle I PWY-6151 2.5.1.6
superpathway of L-cysteine biosynthesis (mammalian) PWY-6292 2.5.1.6
L-methionine salvage cycle II (plants) PWY-7270 2.5.1.6
L-methionine salvage cycle III PWY-7527 2.5.1.6
L-methionine salvage cycle I (bacteria and plants) PWY-7528 2.5.1.6
L-cysteine biosynthesis VI (from L-methionine) PWY-I9 2.5.1.6
S-adenosyl-L-methionine biosynthesis SAM-PWY 2.5.1.6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSEKGRLFTS ESVTEGHPDK ICDAISDSVL DALLAADPRS RVAVETLVTT GQVHVVGEVT TSAKEAFADI TNTVRARILE IGYDSSDKGF DGATCGVNIG IGAQSPDIAQ GVDTAHEARV EGAADPLDSQ GAGDQGLMFG YAINATPELM PLPIALAHRL SRRLTEVRKN GVLPYLRPDG KTQVTIAYED NVPVRLDTVV ISTQHAADID LEKTLDPDIR EKVLNTVLDD LAHETLDAST VRVLVNPTGK FVLGGPMGDA GLTGRKIIVD TYGGWARHGG GAFSGKDPSK VDRSAAYAMR WVAKNVVAAG LAERVEVQVA YAIGKAAPVG LFVETFGTET EDPVKIEKAI GEVFDLRPGA IIRDLNLLRP IYAPTAAYGH FGRTDVELPW EQLDKVDDLK RAI

NT Sequence
GTGAGCGAAA AGGGTCGGCT GTTTACCAGT GAGTCGGTGA CAGAGGGACA TCCCGACAAG ATCTGTGACG CCATCAGCGA CTCGGTTCTG GACGCGCTTC TAGCGGCGGA CCCGCGCTCA CGTGTCGCGG TCGAGACGCT GGTGACCACC GGGCAGGTGC ACGTGGTGGG TGAGGTGACC ACCTCGGCTA AGGAGGCGTT TGCCGACATC ACCAACACGG TCCGCGCACG GATCCTCGAG ATCGGCTACG ACTCGTCGGA CAAGGGTTTC GACGGGGCGA CCTGCGGGGT GAACATCGGC ATCGGCGCAC AGTCACCCGA CATCGCCCAG GGGGTCGACA CCGCCCACGA GGCCCGGGTC GAGGGCGCGG CCGATCCGCT GGACTCCCAG GGCGCCGGTG ACCAGGGCCT GATGTTCGGC TACGCGATCA ATGCCACCCC GGAACTGATG CCACTGCCCA TCGCGCTGGC CCACCGACTG TCGCGGCGGC TGACCGAGGT CCGCAAGAAC GGGGTGCTGC CCTACCTGCG TCCGGATGGC AAGACGCAGG TCACTATCGC CTACGAGGAC AACGTTCCGG TGCGGCTGGA TACCGTGGTC ATCTCCACCC AGCACGCGGC CGATATCGAC CTGGAGAAGA CGCTTGATCC CGACATCCGG GAAAAGGTGC TCAACACCGT GCTCGACGAC CTGGCCCACG AAACCCTGGA CGCGTCGACG GTGCGGGTGC TGGTGAACCC GACCGGCAAG TTCGTGCTCG GCGGGCCGAT GGGCGATGCC GGGCTCACCG GCCGCAAGAT CATCGTCGAC ACCTACGGCG GCTGGGCCCG CCACGGCGGC GGCGCCTTCT CCGGCAAGGA TCCGTCCAAG GTGGACCGGT CGGCGGCGTA CGCGATGCGC TGGGTGGCCA AGAATGTCGT CGCCGCCGGG TTGGCTGAAC GGGTCGAGGT GCAGGTGGCC TACGCCATCG GTAAAGCGGC ACCCGTCGGC CTGTTCGTCG AGACGTTCGG TACCGAGACG GAAGACCCGG TCAAGATCGA GAAGGCCATC GGCGAGGTAT TCGACCTGCG CCCCGGTGCC ATCATCCGCG ACCTGAACCT GTTGCGCCCG ATCTATGCGC CGACCGCCGC CTACGGGCAC TTCGGCCGCA CCGACGTCGA ATTACCGTGG GAGCAGCTCG ACAAGGTCGA CGACCTCAAG CGCGCCATC

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