MyulA.17208.a: RNA polymerase sigma factor
Target Characteristics
from organism:
Mycobacterium ulcerans Agy99
most recent status:
purified
center reference id:
MyulA.17208.a
is community request:
True
associated disease:
Buruli, Bairnsdale ulcer
NIH risk group:
2
is select agent:
False
NIH priority
IIIC
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
CENTER
DOMAIN/REGION
INFO
AA
AA
ORDER
MyulA.17208.a.B1.GE39186
full length
more 1
216
GE39186
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER
DOMAIN/REGION
INFO
AA
AA
ORDER
MyulA.17208.a.B1.PW37715
full length
more 1
216
PW37715
External Resources Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MADIDGVTLA EAPDPEPPEE TDEQLTACFE RDAIPLLDQL YGGALRMTRN PADAEDLLQE TMVKAYAGFR SFREGTNLKA WLYRILTNTY INSYRKKQRQ PAEYPTDEIT DWQLASNAEH SSTGLRSAEV EALEALPDTE IKEALQALPE EFRMAVYYAD VEGFPYKEIA EIMDTPIGTV MSRLHRGRRQ LRTLLADVAK ERGFVRGEQA HQEVSS
NT Sequence
atggccgaca tcgatggtgt gaccctggct gaggcgcccg acccggagcc gcctgaggag actgacgagc agctgactgc gtgcttcgag cgcgacgcga ttcccctgtt ggaccagctg tacggcggcg cgctgcggat gacgcgcaat ccggccgatg ccgaggatct gctgcaggag accatggtca aggcctacgc gggttttcgt tctttccggg agggcaccaa tctcaaagcg tggctgtacc ggattctgac caatacctac atcaacagct atcgcaagaa gcagcgacag cccgcggaat atcccaccga cgagatcacc gattggcagc tggcatccaa cgccgagcat tcctcgaccg ggctgcgttc ggccgaggtc gaggccctcg aggcgttgcc ggacactgag ataaaagagg cgctgcaggc attgccggaa gaattccgga tggcggtcta ctacgccgac gtggagggtt ttccctacaa ggagatcgcc gagatcatgg acacgccgat cgggactgtg atgtcgcggc tacaccgggg cagacggcag ctgcgcaccc tgttggcgga tgtggccaag gaacgtggct tcgtccgcgg tgagcaggcc caccaggagg tgtcttcatg a
purified on: 5/27/2015
concentration: 25mg/ml
observed molecular weight:
28kDa
expression level:
Moderate Expression
protein purification buffer:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
expression host:
data unavailable
vial count (approx.):
8
vial volume:
200µl
percent identity: 100
percent coverage: 100
MAHHHHHHMA DIDGVTLAEA PDPEPPEETD EQLTACFERD AIPLLDQLYG GALRMTRNPA DAEDLLQETM VKAYAGFRSF REGTNLKAWL YRILTNTYIN SYRKKQRQPA EYPTDEITDW QLASNAEHSS TGLRSAEVEA LEALPDTEIK EALQALPEEF RMAVYYADVE GFPYKEIAEI MDTPIGTVMS RLHRGRRQLR TLLADVAKER GFVRGEQAHQ EVSS
ATCTTACTCA CTTATGAAGA CACCTCCTGG TGGGCCTGCT CACCGCGGAC GAAGCCACGT TCCTTGGCCA CATCCGCCAA CAGGGTGCGC AGCTGCCGTC TGCCCCGGTG TAGCCGCGAC ATCACAGTCC CGATCGGCGT GTCCATGATC TCGGCGATCT CCTTGTAGGG AAAACCCTCC ACGTCGGCGT AGTAGACCGC CATCCGGAAT TCTTCCGGCA ATGCCTGCAG CGCCTCTTTT ATCTCAGTGT CCGGCAACGC CTCGAGGGCC TCGACCTCGG CCGAACGCAG CCCGGTCGAG GAATGCTCGG CGTTGGATGC CAGCTGCCAA TCGGTGATCT CGTCGGTGGG ATATTCCGCG GGCTGTCGCT GCTTCTTGCG ATAGCTGTTG ATGTAGGTAT TGGTCAGAAT CCGGTACAGC CACGCTTTGA GATTGGTGCC CTCCCGGAAA GAACGAAAAC CCGCGTAGGC CTTGACCATG GTCTCCTGCA GCAGATCCTC GGCATCGGCC GGATTGCGCG TCATCCGCAG CGCGCCGCCG TACAGCTGGT CCAACAGGGG AATCGCGTCG CGCTCGAAGC ACGCAGTCAG CTGCTCGTCA GTCTCCTCAG GCGGCTCCGG GTCGGGCGCC TCAGCCAGGG TCACACCATC GATGTCGGCC ATATGGTGGT GGTGGTGGTG AGCCAT
MAHHHHHHMA DIDGVTLAEA PDPEPPEETD EQLTACFERD AIPLLDQLYG GALRMTRNPA DAEDLLQETM VKAYAGFRSF REGTNLKAWL YRILTNTYIN SYRKKQRQPA EYPTDEITDW QLASNAEHSS TGLRSAEVEA LEALPDTEIK EALQALPEEF RMAVYYADVE GFPYKEIAEI MDTPIGTVMS RLHRGRRQLR TLLADVAKER GFVRGEQAHQ EVSS
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG ACATCGATGG TGTGACCCTG GCTGAGGCGC CCGACCCGGA GCCGCCTGAG GAGACTGACG AGCAGCTGAC TGCGTGCTTC GAGCGCGACG CGATTCCCCT GTTGGACCAG CTGTACGGCG GCGCGCTGCG GATGACGCGC AATCCGGCCG ATGCCGAGGA TCTGCTGCAG GAGACCATGG TCAAGGCCTA CGCGGGTTTT CGTTCTTTCC GGGAGGGCAC CAATCTCAAA GCGTGGCTGT ACCGGATTCT GACCAATACC TACATCAACA GCTATCGCAA GAAGCAGCGA CAGCCCGCGG AATATCCCAC CGACGAGATC ACCGATTGGC AGCTGGCATC CAACGCCGAG CATTCCTCGA CCGGGCTGCG TTCGGCCGAG GTCGAGGCCC TCGAGGCGTT GCCGGACACT GAGATAAAAG AGGCGCTGCA GGCATTGCCG GAAGAATTCC GGATGGCGGT CTACTACGCC GACGTGGAGG GTTTTCCCTA CAAGGAGATC GCCGAGATCA TGGACACGCC GATCGGGACT GTGATGTCGC GGCTACACCG GGGCAGACGG CAGCTGCGCA CCCTGTTGGC GGATGTGGCC AAGGAACGTG GCTTCGTCCG CGGTGAGCAG GCCCACCAGG AGGTGTCTTC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
