NIAID Emerging and Re-emerging Infectious Diseases NIAID Bioinformatics Resource Centers NIAID Structural Genomic Centers Center for Structural Genomics of Infectious Disease
Seattle Structural Genomics Center for Infectious Disease

NegoA.01161.a: pyruvate kinase

Target Characteristics

from organism: Neisseria gonorrhoeae NCCP11945
most recent status: cloned
center reference id: NegoA.01161.a
is community request: True
associated disease:
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:
I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
NegoA.01161.a.B1.GE40045 full length 1 490 GE40045
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

Method Results
comparative modelling Robetta_85042

External Resources

Resource Reference ID
PATRIC ID: fig|521006.8.peg.2556
UniProt: B4RPV5

Enzyme & Pathway Information

Pathway Pathway ID EC Number
homolactic fermentation ANAEROFRUCAT-PWY 2.7.1.40
glycolysis III (from glucose) ANAGLYCOLYSIS-PWY 2.7.1.40
respiration (anaerobic) ANARESP1-PWY 2.7.1.40
mixed acid fermentation FERMENTATION-PWY 2.7.1.40
glycolysis I (from glucose 6-phosphate) GLYCOLYSIS 2.7.1.40
superpathway of glycolysis and the Entner-Doudoroff pathway GLYCOLYSIS-E-D 2.7.1.40
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass GLYCOLYSIS-TCA-GLYOX-BYPASS 2.7.1.40
superpathway of hexitol degradation (bacteria) HEXITOLDEGSUPER-PWY 2.7.1.40
Entner-Doudoroff pathway II (non-phosphorylative) NPGLUCAT-PWY 2.7.1.40
heterolactic fermentation P122-PWY 2.7.1.40
Bifidobacterium shunt P124-PWY 2.7.1.40
glycolysis V (Pyrococcus) P341-PWY 2.7.1.40
superpathway of N-acetylneuraminate degradation P441-PWY 2.7.1.40
hexitol fermentation to lactate, formate, ethanol and acetate P461-PWY 2.7.1.40
glycolysis IV (plant cytosol) PWY-1042 2.7.1.40
Entner-Doudoroff pathway III (semi-phosphorylative) PWY-2221 2.7.1.40
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle PWY-5464 2.7.1.40
glycolysis II (from fructose 6-phosphate) PWY-5484 2.7.1.40
Rubisco shunt PWY-5723 2.7.1.40
gluconeogenesis II (Methanobacterium thermoautotrophicum) PWY-6142 2.7.1.40
Methanobacterium thermoautotrophicum biosynthetic metabolism PWY-6146 2.7.1.40
glycolysis VI (metazoan) PWY66-400 2.7.1.40
1-butanol autotrophic biosynthesis (engineered) PWY-6886 2.7.1.40
superpathway of glucose and xylose degradation PWY-6901 2.7.1.40
glycerol degradation to butanol PWY-7003 2.7.1.40
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) PWY-7218 2.7.1.40
superpathway of anaerobic sucrose degradation PWY-7345 2.7.1.40
anaerobic energy metabolism (invertebrates, cytosol) PWY-7383 2.7.1.40
superpathway of anaerobic energy metabolism (invertebrates) PWY-7389 2.7.1.40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MNQTSRDLTR ISHNTKIVAT LGPGSNNVEL LEDMIRVGGL NVVRFNFSHG TPEFHQENAR IVREAAKRAG QEIAIIADLQ GPKIRVGKIA GGGIELNKGE TLVLDAALEG EGTREAVGLD YRDLPDDVAA GDVLWLDDGL LTLTVESVEG SRIITRVENS HILKSNKGIN KRGGGLSAGA LTEKDFRDLK TAIAIGCDYL AISFVKSAED LHIARAKVEE EMKGSTAVRP GLVSKIERVE AIENLDEIIL AGDGIMVARG DLAVEVGHAA VPALQKRMIR RARELRRFSI TATQMMESMI TNPVPTRAEV SDVANAVLDG TDAVMCSAET AVGAYPFETV SQMAIICAAA EKEQDSLNGV AEQTEYPEAV STNLAIAGGA VSVARAVHAK AIVALTESGS TAFEISRHNI TLPIFALTPS VSAQRRMAMY RGVRPLILAT STDHDTALNE VETMLVEHNI LHSGDQYIIT SGSQMRESGS TNTLEVLRVK

NT Sequence
ATGAACCAGA CAAGCCGCGA TCTGACCCGC ATCAGCCACA ACACCAAAAT CGTCGCCACC CTTGGGCCGG GCAGCAACAA CGTCGAACTG TTGGAAGACA TGATCCGCGT CGGCGGCCTG AACGTCGTCC GCTTCAACTT CAGCCACGGC ACGCCCGAAT TCCATCAGGA AAACGCCCGC ATCGTGCGCG AGGCGGCAAA ACGCGCCGGA CAGGAAATCG CCATCATTGC CGATCTGCAG GGCCCGAAAA TCCGCGTGGG CAAAATCGCC GGCGGCGGCA TCGAATTGAA CAAAGGCGAA ACATTGGTAC TCGATGCCGC GCTCGAAGGC GAAGGCACGC GCGAGGCGGT CGGTTTGGAC TACCGCGACC TGCCCGACGA CGTTGCCGCA GGCGATGTCT TGTGGCTGGA CGACGGCCTG CTGACCCTGA CCGTGGAATC CGTCGAAGGC AGCAGGATTA TCACAAGGGT GGAAAACAGC CACATCCTGA AAAGCAACAA AGGCATCAAC AAACGCGGCG GCGGTTTGTC CGCAGGCGCG TTGACCGAAA AAGACTTCCG CGACCTGAAA ACCGCGATTG CCATCGGTTG CGACTATCTC GCCATCAGCT TTGTGAAATC CGCCGAAGAC CTGCACATCG CGCGCGCCAA AGTCGAAGAG GAAATGAAGG GCAGCACTGC CGTCCGCCCC GGTTTGGTTT CCAAAATCGA ACGCGTAGAA GCGATTGAAA ACCTTGATGA AATCATCCTT GCCGGCGACG GCATTATGGT TGCGCGCGGC GACTTGGCGG TCGAAGTCGG ACACGCCGCC GTCCCCGCCC TGCAAAAACG GATGATCCGC CGCGCCCGCG AGTTGCGCCG CTTCAGCATT ACGGCGACGC AAATGATGGA ATCGATGATT ACCAACCCCG TGCCGACCCG CGCGGAAGTC AGCGATGTGG CAAACGCGGT ATTGGACGGC ACCGATGCGG TGATGTGTTC CGCCGAAACC GCCGTCGGCG CGTATCCGTT TGAAACCGTC AGCCAAATGG CGATTATCTG CGCGGCTGCG GAAAAAGAAC AGGATTCGCT CAACGGCGTT GCCGAACAGA CAGAGTATCC CGAAGCCGTC AGCACCAACT TAGCGATTGC CGGCGGCGCG GTCAGCGTGG CGCGCGCGGT TCACGCCAAA GCCATCGTCG CCCTGACCGA AAGCGGTTCG ACCGCCTTTG AAATCAGCCG CCACAATATT ACCCTGCCGA TTTTCGCGCT GACCCCGAGC GTTTCCGCCC AACGCCGTAT GGCGATGTAC CGGGGCGTGC GCCCGCTGAT TTTGGCAACC AGCACCGACC ACGACACGGC GTTGAACGAA GTCGAAACGA TGCTGGTGGA ACACAACATC CTGCATTCCG GCGACCAGTA CATCATCACC AGCGGCTCGC AAATGCGCGA ATCCGGTTCG ACCAATACGC TGGAAGTGCT GCGCGTCAAA TAA

© 2020 Seattle Children's Research Institute. All rights reserved.